AB137130

Anti-TNNC1 antibody [EPR9692(B)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

|

(8 Publications)

Rabbit Recombinant Monoclonal TNNC1 antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

TNNC, TNNC1, TN-C

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis of paraffin embedded Human heart tissue labelling TNNC1 using ab137130 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling TNNC1 using ab137130 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing positive staining in human Heart tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing negative staining in Normal human kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing positive staining in human Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing negative staining in Normal human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

WB

Unknown

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

All lanes:

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

WB

Lab

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab213204 was used as a loading control.

All lanes:

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with TNNC1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Exposure time: 80s

Carrier free

Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9692(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components : Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

See full target information TNNC1

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:987-1002 PubMed39196031

2024

Cardiac troponin I directly binds and inhibits mitochondrial ATP synthase with a noncanonical role in the post-ischemic heart.

Applications

Unspecified application

Species

Unspecified reactive species

Aly Elezaby,Amanda J Lin,Vijith Vijayan,Suman Pokhrel,Benjamin R Kraemer,Luiz R G Bechara,Isabel Larus,Junhui Sun,Valentina Baena,Zulfeqhar A Syed,Elizabeth Murphy,Brian Glancy,Nicolai P Ostberg,Bruno B Queliconi,Juliane C Campos,Julio C B Ferreira,Bereketeab Haileselassie,Daria Mochly-Rosen

Basic research in cardiology 118:20 PubMed37212935

2023

SMYD1a protects the heart from ischemic injury by regulating OPA1-mediated cristae remodeling and supercomplex formation.

Applications

Unspecified application

Species

Unspecified reactive species

Marta W Szulik,Steven Valdez,Maureen Walsh,Kathryn Davis,Ryan Bia,Emilee Horiuchi,Sean O'Very,Anil K Laxman,Linda Sandaklie-Nicolova,David R Eberhardt,Jessica R Durrant,Hanin Sheikh,Samuel Hickenlooper,Magnus Creed,Cameron Brady,Mickey Miller,Li Wang,June Garcia-Llana,Christopher Tracy,Stavros G Drakos,Katsuhiko Funai,Dipayan Chaudhuri,Sihem Boudina,Sarah Franklin

Life (Basel, Switzerland) 12: PubMed36013400

2022

Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Sultan,Ernest Adeghate,Bright Starling Emerald,Muhammad A Qureshi,Saeed Tariq Minhas,Frank Christopher Howarth

Communications biology 4:703 PubMed34103648

2021

Random errors in protein synthesis activate an age-dependent program of muscle atrophy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

James Moore,Rashid Akbergenov,Martina Nigri,Patricia Isnard-Petit,Amandine Grimm,Petra Seebeck,Lisa Restelli,Stephan Frank,Anne Eckert,Kader Thiam,David P Wolfer,Dimitri Shcherbakov,Erik C Böttger

The Veterinary record 185:694 PubMed31554713

2019

Stress cardiomyopathy in stranded cetaceans: a histological, histochemical and immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Nakita Câmara,Eva Sierra,Carolina Fernández-Maldonado,Antonio Espinosa de Los Monteros,Manuel Arbelo,Antonio Fernández,Pedro Herráez

Journal of visualized experiments : JoVE : PubMed31355804

2019

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhao,Yawen Tang,Patrick J Ernst,Asher Kahn-Krell,Chengming Fan,Danielle Pretorius,Hanxi Zhu,Xi Lou,Lufang Zhou,Jianyi Zhang,Wuqiang Zhu

Journal of comparative physiology. B, Biochemical, 188:863-876 PubMed30039299

2018

Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Chang,Tingyun Lei,Xiufeng Ma,Jie Zhang,Huiping Wang,Xingyuan Zhang,Yun-Fang Gao

Proteome science 14:16 PubMed27833457

2016

iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels ().

Applications

WB

Species

Unspecified reactive species

Hui Chang,Shan-Feng Jiang,Kai Dang,Hui-Ping Wang,Shen-Hui Xu,Yun-Fang Gao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com