Anti-TNNC1 antibody [EPR9692(B)] (ab137130)

Rabbit Recombinant Monoclonal TNNC1 antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF
Human	Tested	Not recommended	Tested	Not recommended
Mouse	Predicted	Not recommended	Expected	Not recommended
Rat	Predicted	Not recommended	Expected	Not recommended
Transfected cell lysate - Human	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -
Species Transfected cell lysate - Human	Dilution info 1/1000 - 1/10000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Target data

See full target information TNNC1

Function

Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

Alternative names

TNNC, TNNC1, TN-C

Recommended products

Rabbit Recombinant Monoclonal TNNC1 antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR9692(B)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
All lanes: Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution
Lane 1: Fetal heart lysate at 10 µg
Lane 2: Human skeletal muscle lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Immunohistochemical analysis of paraffin embedded Human heart tissue labelling TNNC1 using ab137130 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling TNNC1, using ab137130 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Immunohistochemical analysis using ab137130 showing positive staining in human Heart tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Immunohistochemical analysis using ab137130 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Immunohistochemical analysis using ab137130 showing negative staining in Normal human kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Immunohistochemical analysis using ab137130 showing negative staining in Normal human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Immunohistochemical analysis using ab137130 showing positive staining in human Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as a loading control.
All lanes: Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with TNNC1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
Exposure time: 80s