JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248838

Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TNNC1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples.

View Alternative Names

TNNC, TNNC1, TN-C

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human heart tissue labelling Cardiac Troponin C using ab137130 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling Cardiac Troponin C, using ab137130 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab137130 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab137130 showing positive staining in human Heart tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab137130 showing negative staining in Normal human kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab137130 showing positive staining in human Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab137130 showing negative staining in Normal human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • WB

Unknown

Western blot - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (<a href='/en-us/products/primary-antibodies/tnnc1-antibody-epr9692b-ab137130'>ab137130</a>) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Western blot - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)
  • WB

Lab

Western blot - Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free (AB248838)

This data was developed using ab137130, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab213204 was used as a loading control.

All lanes:

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (<a href='/en-us/products/primary-antibodies/tnnc1-antibody-epr9692b-ab137130'>ab137130</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with TNNC1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Exposure time: 80s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9692(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab248838 is the carrier-free version of ab137130.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components : Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
See full target information TNNC1
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB314712

Human Troponin C ELISA Kit

Sandwich ELISA - Human Troponin C ELISA Kit (AB314712)

  • 0
  • 0
Primary Antibodies

AB47003

Anti-Cardiac Troponin I antibody

BOND RX™ Validated

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

  • 5
  • 17
Primary Antibodies

AB45932

Anti-Cardiac Troponin T antibody

BOND RX™ Validated

Immunohistochemistry - Anti-Cardiac Troponin T antibody (AB45932)

  • 4
  • 7
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Recombinant|Lab Essentials

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
Primary Antibodies

AB72230

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

  • 4
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com