Anti-TNNI1 antibody [EPR17120-11]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] (AB203515)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TNNI1 with ab203515 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. No staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] (AB203515)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TNNI1 with ab203515 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on striated Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-TNNI1 antibody [EPR17120-11] (AB203515)

TNNI1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab203515 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203515 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal heart whole cell lysate 10 μg (Input). Lane 2 : ab203515 IP in Human fetal heart whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203515 in Human fetal heart whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-TNNI1 antibody [EPR17120-11] (ab203515)

Predicted band size: 22 kDa

false

• WB

Supplier Data

Western blot - Anti-TNNI1 antibody [EPR17120-11] (AB203515)

Blocking/Dilution buffer : 5% NFDM/TBST.

TNNI1 is abundantly expressed in the human skeletal muscle and heart.

All lanes:

Western blot - Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-TNNI1 antibody [EPR17120-11] (AB203515)

Blocking/Dilution buffer : 5% NFDM/TBST.

TNNI1 is abundantly expressed in the human skeletal muscle and heart.

All lanes:

Western blot - Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution

All lanes:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Exposure time: 5s