Rabbit Recombinant Monoclonal TNPO3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.
Images
![Western blot - Anti-TNPO3 antibody [EPR5264] (AB109386), expandable thumbnail](https://content.abcam.com/products/images/tnpo3-antibody-epr5264-ab109386--western-blot-img125538.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TNPO3 antibody [EPR5264] (AB109386)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNPO3 antibody [EPR5264] (AB109386), expandable thumbnail](https://content.abcam.com/products/images/tnpo3-antibody-epr5264-ab109386--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109690.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNPO3 antibody [EPR5264] (AB109386)")
![OI-RD Scanning - Anti-TNPO3 antibody [EPR5264] (AB109386), expandable thumbnail](https://content.abcam.com/products/images/tnpo3-antibody-epr5264-ab109386--oi-rd-scanning-img83997.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-TNPO3 antibody [EPR5264] (AB109386)")
Publications
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- Nature communications 12:68292021Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergi Marco,Matthew Neilson,Madeleine Moore,Arantxa Perez-Garcia,Holly Hall,Louise Mitchell,Sergio Lilla,Giovani R Blanco,Ann Hedley,Sara Zanivan,Jim C NormanPubMed 34819513
- Cell reports 29:3983-3996.e42019Cellular IP Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP Are Attenuated for Infection and Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonna L Mallery,K M Rifat Faysal,Alex Kleinpeter,Miranda S C Wilson,Marina Vaysburd,Adam J Fletcher,Mariia Novikova,Till Böcking,Eric O Freed,Adolfo Saiardi,Leo C JamesPubMed 31851928
- Journal of virology 93:2019Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAurore Bochnakian et. al.PubMed 31341054
- Neurology. Genetics 5:e3372019Novel mutation in causes congenital limb-girdle myopathy with slow progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Vihola et. al.PubMed 31192305
- PLoS pathogens 10:e10039692014A cyclophilin homology domain-independent role for Nup358 in HIV-1 infection.
- PloS one 8:e635362013Next-generation sequencing identifies transportin 3 as the causative gene for LGMD1F.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Importin, which transports target proteins into the nucleus (PubMed:10366588, PubMed:10713112, PubMed:11517331, PubMed:12628928, PubMed:24449914). Specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1 and SFRS2, by recognizing phosphorylated SR domains (PubMed:10366588, PubMed:10713112, PubMed:11517331, PubMed:12628928, PubMed:24449914). Also mediates the nuclear import of serine/arginine (SR) protein CPSF6, independently of CPSF6 phosphorylation (PubMed:30916345, PubMed:31465518). The nuclear import process is regulated by the small GTPase Ran that partitions between cytoplasm and nucleus in the predominantly GDP- and GTP-bound form, respectively (PubMed:23878195, PubMed:24449914). Importin associates with target cargo proteins in the cytoplasm, and the competitive binding of GTP-bound Ran induces the release of cargos in the nucleus (PubMed:23878195, PubMed:24449914). (Microbial infection) Involved in immunodeficiency virus (HIV-1) infection by importing the pre-integration complex (PIC) into the nucleus (PubMed:18722123, PubMed:21901095, PubMed:22398280, PubMed:29329553). Required for a nuclear maturation step of HIV-1 prior to integration (PubMed:21901095, PubMed:22398280).
Alternative names
IPO12, TNPO3, Transportin-3, Importin-12, Transportin-SR, Imp12, TRN-SR
Recommended products
Rabbit Recombinant Monoclonal TNPO3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5264
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 4.7 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-TNPO3 antibody [EPR5264] (ab109386), expandable thumbnail](https://content.abcam.com/products/images/tnpo3-antibody-epr5264-ab109386--western-blot-img125538.jpg "Western blot - Anti-TNPO3 antibody [EPR5264] (ab109386)")
Western blot - Anti-TNPO3 antibody [EPR5264] (ab109386)
All lanes: Western blot - Anti-TNPO3 antibody [EPR5264] (ab109386) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: Ramos cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Predicted band size: 104 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNPO3 antibody [EPR5264] (ab109386), expandable thumbnail](https://content.abcam.com/products/images/tnpo3-antibody-epr5264-ab109386--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109690.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNPO3 antibody [EPR5264] (ab109386)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNPO3 antibody [EPR5264] (ab109386)
ab109386, at 1/100 dilution, staining TNPO3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-TNPO3 antibody [EPR5264] (ab109386), expandable thumbnail](https://content.abcam.com/products/images/tnpo3-antibody-epr5264-ab109386--oi-rd-scanning-img83997.jpg "OI-RD Scanning - Anti-TNPO3 antibody [EPR5264] (ab109386)")
OI-RD Scanning - Anti-TNPO3 antibody [EPR5264] (ab109386)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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