AB192247

Anti-TNS4/CTEN antibody [EPR13792]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal TNS4/CTEN antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

CTEN, PP14434, TNS4, Tensin-4, C-terminal tensin-like protein

1 Images

Lab

Western blot - Anti-TNS4/CTEN antibody [EPR13792] (AB192247)

Western blot : Anti-TNS4/CTEN antibody [EPR13792] ab192247 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80 kDa in Wild-type A549 cell lysates with no signal observed at this size in TNS4 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TNS4/CTEN antibody [EPR13792] (ab192247) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

TNS4 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 80 kDa,37 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13792

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes EGF-induced cell migration by displacing tensin TNS3 from the cytoplasmic tail of integrin ITGB1 which results in dissociation of TNS3 from focal adhesions, disassembly of actin stress fibers and initiation of cell migration (PubMed : 17643115). Suppresses ligand-induced degradation of EGFR by reducing EGFR ubiquitination in the presence of EGF (PubMed : 23774213). Increases MET protein stability by inhibiting MET endocytosis and subsequent lysosomal degradation which leads to increased cell survival, proliferation and migration (PubMed : 24814316).

See full target information TNS4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:115038 PubMed39630581

2024

OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase.

Applications

Unspecified application

Species

Unspecified reactive species

Praveenraj Elancheliyan,Klaudia K Maruszczak,Remigiusz Adam Serwa,Till Stephan,Ahmet Sadik Gulgec,Mayra A Borrero-Landazabal,Sonia Ngati,Aleksandra Gosk,Stefan Jakobs,Michal Wasilewski,Agnieszka Chacinska

Genome medicine 13:58 PubMed33853662

2021

PRMT1-mediated H4R3me2a recruits SMARCA4 to promote colorectal cancer progression by enhancing EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yao,Tao Gui,Xiangwei Zeng,Yexuan Deng,Zhi Wang,Ying Wang,Dongjun Yang,Qixiang Li,Peipei Xu,Ruifeng Hu,Xinyu Li,Bing Chen,Jin Wang,Ke Zen,Haitao Li,Melissa J Davis,Marco J Herold,Hua-Feng Pan,Zhi-Wei Jiang,David C S Huang,Ming Liu,Junyi Ju,Quan Zhao

Oncology letters 21:40 PubMed33262832

2020

Overexpression of CTEN is associated with gefitinib resistance in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangdong Lu,Yao Zhang,Yukai Pan,Minmin Cao,Xie Zhou,Tingrong Zhang

Cell death & disease 11:597 PubMed32732965

2020

The miR-1224-5p/TNS4/EGFR axis inhibits tumour progression in oesophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Zhou Shi,Wen-Jun Wang,Yun-Xia Chen,Ze-Wen Fan,Xiu-Feng Xie,Li-Yan Yang,Chen Chang,Yan Cai,Jia-Jie Hao,Ming-Rong Wang,Jie Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNS4/CTEN antibody [EPR13792]

Western blot - Anti-TNS4/CTEN antibody [EPR13792] (AB192247)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise