Rabbit Recombinant Monoclonal TNS4/CTEN antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (AB99887), expandable thumbnail](https://content.abcam.com/products/images/tns4-cten-antibody-sp83-ab99887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (AB99887)")
![Flow Cytometry (Intracellular) - Anti-TNS4/CTEN antibody [SP83] (AB99887), expandable thumbnail](https://content.abcam.com/products/images/tns4-cten-antibody-sp83-ab99887--flow-cytometry-intracellular-img37504.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TNS4/CTEN antibody [SP83] (AB99887)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (AB99887), expandable thumbnail](https://content.abcam.com/products/images/tns4-cten-antibody-sp83-ab99887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110962.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (AB99887)")
Publications
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- Technology in cancer research & treatment 20:153303382110455062021CTEN Inhibits Tumor Angiogenesis and Growth by Targeting VEGFA Through Down-Regulation of β-Catenin in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangdong Lu,Bin Zhou,Minmin Cao,Qin Shao,Yukai Pan,Tao ZhaoPubMed 34817293
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Promotes EGF-induced cell migration by displacing tensin TNS3 from the cytoplasmic tail of integrin ITGB1 which results in dissociation of TNS3 from focal adhesions, disassembly of actin stress fibers and initiation of cell migration (PubMed:17643115). Suppresses ligand-induced degradation of EGFR by reducing EGFR ubiquitination in the presence of EGF (PubMed:23774213). Increases MET protein stability by inhibiting MET endocytosis and subsequent lysosomal degradation which leads to increased cell survival, proliferation and migration (PubMed:24814316).
Alternative names
CTEN, PP14434, TNS4, Tensin-4, C-terminal tensin-like protein
Recommended products
Rabbit Recombinant Monoclonal TNS4/CTEN antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP83
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887), expandable thumbnail](https://content.abcam.com/products/images/tns4-cten-antibody-sp83-ab99887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122180.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling TNS4/CTEN with Purified ab99887 at 1/100 dilution (0.92 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Flow Cytometry (Intracellular) - Anti-TNS4/CTEN antibody [SP83] (ab99887), expandable thumbnail](https://content.abcam.com/products/images/tns4-cten-antibody-sp83-ab99887--flow-cytometry-intracellular-img37504.jpg "Flow Cytometry (Intracellular) - Anti-TNS4/CTEN antibody [SP83] (ab99887)")
Flow Cytometry (Intracellular) - Anti-TNS4/CTEN antibody [SP83] (ab99887)
Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling TNS4/CTEN with purified ab99887 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control -Unlabelled cells (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887), expandable thumbnail](https://content.abcam.com/products/images/tns4-cten-antibody-sp83-ab99887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110962.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)
Staining of Human TNS4/CTEN in a Formalin/PFA-fixed paraffin-embedded sections of Human Liver Carcinoma using ab99887 at a dilution of 1/100.
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