AB180514

Anti-TOB2 antibody [EPR12588] - N-terminal

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal TOB2 antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA1663, TOB4, TROB2, TOB2, Protein Tob2, Protein Tob4, Transducer of erbB-2 2

2 Images

Supplier Data

Immunoprecipitation - Anti-TOB2 antibody [EPR12588] - N-terminal (AB180514)

Immunoprecipitation analysis of K562 cell lysate labeling TOB2 using ab180514 at 1/50 dilution in Lane 1. Lane 2 is the negative control. A rabbit anti rabbit IgG HRP was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-TOB2 antibody [EPR12588] - N-terminal (ab180514)

Predicted band size: 37 kDa

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Supplier Data

Western blot - Anti-TOB2 antibody [EPR12588] - N-terminal (AB180514)

All lanes:

Western blot - Anti-TOB2 antibody [EPR12588] - N-terminal (ab180514) at 1/20000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Human skeletal cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12588

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Anti-proliferative protein inhibits cell cycle progression from the G0/G1 to S phases.

See full target information TOB2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8016 PubMed40866357

2025

The balance between B55α and Greatwall expression levels predicts sensitivity to Greatwall inhibition in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Róbert Zach,Michael Annis,Sandra M Martin-Guerrero,Abdulrahman Alatawi,Kim Hou Chia,Megan Meredith,Kay Osborn,Nisha Peter,William Pearce,Jessica Booth,Mohan Rajasekaran,Samantha Dias,Lily Coleman-Evans,William R Foster,Jon A Harper,Alex D Herbert,Catherine Tighe,Tristan Reuillon,Ryan West,Oliver Busby,Kamila Burdova,Damien Crepin,Sergi Ortoll,Kulthida Vaeteewoottacharn,Donniphat Dejsuphong,John Spencer,Hitesh Patel,Darren Le Grand,Thomas A Hunt,David M Andrews,Hiroyuki Yamano,Pedro R Cutillas,Antony W Oliver,Simon E Ward,Helfrid Hochegger

Journal of immunology (Baltimore, Md. : 1950) 205:981-986 PubMed32611726

2020

Tob2 Inhibits TLR-Induced Inflammatory Responses by Association with TRAF6 and MyD88.

Applications

Unspecified application

Species

Unspecified reactive species

Guosheng Jiang,Mouchun Gong,Hui Song,Wangnan Sun,Wei Zhao,Lijuan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TOB2 antibody [EPR12588] - N-terminal

Immunoprecipitation - Anti-TOB2 antibody [EPR12588] - N-terminal (AB180514)

Western blot - Anti-TOB2 antibody [EPR12588] - N-terminal (AB180514)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise