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AB67310

Anti-Toca-1 antibody

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(1 Publication)

Rabbit Polyclonal Toca-1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FNBP1L.

View Alternative Names

C1orf39, TOCA1, FNBP1L, Formin-binding protein 1-like, Transducer of Cdc42-dependent actin assembly protein 1, Toca-1

1 Images
Western blot - Anti-Toca-1 antibody (AB67310)
  • WB

Unknown

Western blot - Anti-Toca-1 antibody (AB67310)

All lanes:

Western blot - Anti-Toca-1 antibody (ab67310) at 1 µg/mL

Lane 1:

Mouse brain tissue lysate at 15 µg with no blocking peptide

Lane 2:

Mouse brain tissue lysate at 15 µg with blocking peptide

Predicted band size: 70 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human FNBP1L. The exact immunogen used to generate this antibody is proprietary information.

Q5T0N5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>This application in validated in mouse samples.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toca-1 also known as Transducer of Cdc42-dependent actin assembly protein 1 plays a significant role in cellular mechanics. It is part of the FNBP1L gene family with a molecular mass of around 65 kDa. Toca-1 facilitates the reorganization of the cytoskeleton by interacting with Cdc42 and other proteins. It has a widespread expression in various tissues with pronounced levels in the brain indicating its importance in neural functions.
Biological function summary

Toca-1 influences actin polymerization and cellular membrane dynamics. It functions as part of a complex that includes Cdc42 and the Neural Wiskott-Aldrich syndrome protein (N-WASP) both of which are important for the regulation of actin filament assembly and endocytosis. This complex plays a fundamental role in cell movement and shape maintenance important during processes such as cell migration and cytokinesis.

Pathways

Toca-1 participates actively in the regulation of actin cytoskeleton pathways and related cell signaling processes. It is notably involved in the Cdc42 pathway where it connects with proteins such as N-WASP and Arp2/3 complex to drive actin assembly. This connection highlights its relevance in pathways controlling cell motility and morphogenesis processes essential for proper cell functioning and linkage to other signaling cascades.

Toca-1 has been studied in connection with cancer and neurodegenerative diseases. Its interaction with Cdc42 and aberrant signaling in the actin cytoskeleton may contribute to cancer metastasis as altered cell migration and invasion are key aspects of tumor progression. In neurological disorders Toca-1's involvement in synaptic function and plasticity highlights potential roles in neurodegeneration where dysfunctional cytoskeletal dynamics affect neuron signaling and viability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens.
See full target information FNBP1L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 217:3255-3266 PubMed29945904

2018

Actin polymerization controls cilia-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L Drummond,Mischa Li,Eric Tarapore,Tuyen T L Nguyen,Baina J Barouni,Shaun Cruz,Kevin C Tan,Anthony E Oro,Scott X Atwood
View all publications
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Product promise

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