AB187198

Anti-Tollip antibody

Name: Anti-Tollip antibody (ab187198) | Abcam
Brand: Abcam Limited
SKU: AB187198

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(13 Publications)

Rabbit Polyclonal Tollip antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TOLLIP.

View Alternative Names

Toll-interacting protein, TOLLIP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (AB187198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (AB187198)

Paraffin-embedded rat brain tissue stained for Tollip using ab187198 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Tollip antibody (AB187198)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tollip antibody (AB187198)

Immunofluorescence staining of L929 cells stained for Tollip with ab187198 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

Supplier Data

Western blot - Anti-Tollip antibody (AB187198)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Tollip antibody (ab187198) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 25 µg

Lane 2:

OVCAR3 cell lysate at 25 µg

Lane 3:

MCF-7 cell lysate at 25 µg

Lane 4:

293T cell lysate at 25 µg

Lane 5:

Mouse spleen tissue lysate at 25 µg

Lane 6:

Mouse intestine tissue lysate at 25 µg

Lane 7:

Mouse liver tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TOLLIP.

Q9H0E2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tollip also known as Toll-interacting protein acts as an important regulator in the immune response. This protein weighs approximately 30 kDa and is found expressed in several tissues especially in the lung liver and spleen. Tollip binds to various ubiquitinated proteins and plays a role in the endocytosis process assisting in protein trafficking. It functions by modulating the signaling pathways that involve innate immunity primarily by interacting with Toll-like receptors (TLRs).

Biological function summary

Toll-interacting protein participates in the negative regulation of the immune response preventing overactivation that could lead to inflammation. Tollip forms a complex with TLRs and IL-1 receptor pathway proteins to control inflammatory signaling. Through its action Tollip binds to Tom1 another protein that links Tollip to phosphatidylinositol-3-phosphate. By regulating these pathways Tollip assists in maintaining a balanced immune response.

Pathways

The protein engages in the TLR signaling pathway and the IL-1 receptor pathway. Both pathways are key in initiating immune responses against pathogens. Tollip as part of these pathways directly associates with proteins like IRAK-1 where it modulates the activity and ensures controlled signaling. This regulation prevents excessive or unnecessary activation that can result in immune-related damage.

Tollip links to conditions such as inflammatory bowel disease and sepsis. Both conditions involve significant immune dysregulation. In these disease states Tollip interacts with proteins like MyD88 affecting the response to pathogens and inflammatory stimuli. Malfunction or altered expression of Tollip can worsen symptoms or contribute to disease progression due to abnormal immune reactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the signaling pathway of IL-1 and Toll-like receptors (PubMed : 10854325, PubMed : 11751856). Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex (PubMed : 10854325). Inhibits IRAK1 phosphorylation and kinase activity (PubMed : 11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates (PubMed : 25042851). The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed : 25042851). In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed : 15047686). Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed : 26320582).

See full target information TOLLIP

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:133-148 PubMed39735680

2024

CAF-derived exosome-miR-3124-5p promotes malignant biological processes in NSCLC via the TOLLIP/TLR4-MyD88-NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Sun,Qinghua Song,Hua Liu

Science advances 10:eadp3000 PubMed39121224

2024

A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Urbi Mukhopadhyay,Sophie Levantovsky,Teresa Maria Carusone,Sarah Gharbi,Frank Stein,Christian Behrends,Sagar Bhogaraju

Nature communications 14:870 PubMed36797266

2023

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.

Applications

Unspecified application

Species

Unspecified reactive species

Karsten Nalbach,Martina Schifferer,Debjani Bhattacharya,Hung Ho-Xuan,Wei Chou Tseng,Luis A Williams,Alexandra Stolz,Stefan F Lichtenthaler,Zvulun Elazar,Christian Behrends

International heart journal 63:915-927 PubMed36184551

2022

MiRNA-6870-3p Regulates Lipopolysaccharide Induced Epicardial Adipose Tissue Inflammatory Genes via Targeting Tollip-Mediated JNK and NF-κB Signaling in Coronary Artery Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Guo Wu,Wen-Kai Yang,Hao-Zong Huang,Kai Tang,Guang-Di Zheng

Autophagy 19:1332-1347 PubMed36126167

2022

NLRC5 restricts dengue virus infection by promoting the autophagic degradation of viral NS3 through E3 ligase CUL2 (cullin 2).

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Hao,Jinqian Li,Zhenzhen Zhang,Yang Yang,Qing Zhou,Tiantian Wu,Tongling Chen,Zhongdao Wu,Ping Zhang,Jun Cui,Yi-Ping Li

Journal of innate immunity 15:204-221 PubMed36116427

2022

Cathelicidins Induce Toll-Interacting Protein Synthesis to Prevent Apoptosis in Colonic Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Ravi Holani,Chathurika Rathnayaka,Graham A D Blyth,Anshu Babbar,Priyoshi Lahiri,Daniel Young,Antoine Dufour,Morley D Hollenberg,Derek M McKay,Eduardo R Cobo

Life science alliance 5: PubMed36114006

2022

ALS-linked loss of Cyclin-F function affects HSP90.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Siebert,Vanessa Gattringer,Jochen H Weishaupt,Christian Behrends

Bioengineered 13:5005-5020 PubMed35164650

2022

Somatostatin attenuates intestinal epithelial barrier injury during acute intestinal ischemia-reperfusion through Tollip/Myeloiddifferentiationfactor 88/Nuclear factor kappa-B signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Tian,Ruo Shu,Yi Lei,Yu Xu,Xinfeng Zhang,Huayou Luo

Molecular cell 81:1337-1354.e8 PubMed33545068

2021

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Zellner,Martina Schifferer,Christian Behrends

Frontiers in cellular and infection microbiology 10:583137 PubMed33425778

2020

Autophagy Receptor Tollip Facilitates Bacterial Autophagy by Recruiting Galectin-7 in Response to Group A Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Yu Lin,Takashi Nozawa,Atsuko Minowa-Nozawa,Hirotaka Toh,Miyako Hikichi,Junpei Iibushi,Ichiro Nakagawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Tollip antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (AB187198)

Immunocytochemistry/ Immunofluorescence - Anti-Tollip antibody (AB187198)

Western blot - Anti-Tollip antibody (AB187198)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise