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AB223763

Anti-Tollip antibody

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(1 Publication)

Rabbit Polyclonal Tollip antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TOLLIP aa 100-250.

View Alternative Names

Toll-interacting protein, TOLLIP

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tollip antibody (AB223763)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tollip antibody (AB223763)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Tollip (green) using ab223763 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (AB223763)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (AB223763)

Paraffin-embedded human small intestine tissue stained for Tollip using ab223763 at 1/500 dilution in immunohistochemical analysis

Western blot - Anti-Tollip antibody (AB223763)
  • WB

Supplier Data

Western blot - Anti-Tollip antibody (AB223763)

All lanes:

Western blot - Anti-Tollip antibody (ab223763) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-2 OS (human brain glioma cell line) cell lysate

Predicted band size: 30 kDa

false

Western blot - Anti-Tollip antibody (AB223763)
  • WB

Supplier Data

Western blot - Anti-Tollip antibody (AB223763)

All lanes:

Western blot - Anti-Tollip antibody (ab223763) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II whole cell lysate

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TOLLIP aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H0E2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/0.04 - 1/0.4", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tollip also known as Toll-interacting protein acts as an important regulator in the immune response. This protein weighs approximately 30 kDa and is found expressed in several tissues especially in the lung liver and spleen. Tollip binds to various ubiquitinated proteins and plays a role in the endocytosis process assisting in protein trafficking. It functions by modulating the signaling pathways that involve innate immunity primarily by interacting with Toll-like receptors (TLRs).
Biological function summary

Toll-interacting protein participates in the negative regulation of the immune response preventing overactivation that could lead to inflammation. Tollip forms a complex with TLRs and IL-1 receptor pathway proteins to control inflammatory signaling. Through its action Tollip binds to Tom1 another protein that links Tollip to phosphatidylinositol-3-phosphate. By regulating these pathways Tollip assists in maintaining a balanced immune response.

Pathways

The protein engages in the TLR signaling pathway and the IL-1 receptor pathway. Both pathways are key in initiating immune responses against pathogens. Tollip as part of these pathways directly associates with proteins like IRAK-1 where it modulates the activity and ensures controlled signaling. This regulation prevents excessive or unnecessary activation that can result in immune-related damage.

Tollip links to conditions such as inflammatory bowel disease and sepsis. Both conditions involve significant immune dysregulation. In these disease states Tollip interacts with proteins like MyD88 affecting the response to pathogens and inflammatory stimuli. Malfunction or altered expression of Tollip can worsen symptoms or contribute to disease progression due to abnormal immune reactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the signaling pathway of IL-1 and Toll-like receptors (PubMed : 10854325, PubMed : 11751856). Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex (PubMed : 10854325). Inhibits IRAK1 phosphorylation and kinase activity (PubMed : 11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates (PubMed : 25042851). The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed : 25042851). In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed : 15047686). Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed : 26320582).
See full target information TOLLIP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:2179 PubMed35449133

2022

Golgi apparatus-targeted aggregation-induced emission luminogens for effective cancer photodynamic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Minglun Liu,Yuncong Chen,Yan Guo,Hao Yuan,Tongxiao Cui,Shankun Yao,Suxing Jin,Huanhuan Fan,Chengjun Wang,Ran Xie,Weijiang He,Zijian Guo
View all publications
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Product promise

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