AB37155

Anti-Tollip antibody

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(4 Publications)

Rabbit Polyclonal Tollip antibody. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TOLLIP.

View Alternative Names

Toll-interacting protein, TOLLIP

1 Images

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Western blot - Anti-Tollip antibody (AB37155)

Lane 1:

Western blot - Anti-Tollip antibody (ab37155) at 1 µg/mL

Lane 2:

Western blot - Anti-Tollip antibody (ab37155) at 2 µg/mL

All lanes:

Rat brain cell lysate

Predicted band size: 30 kDa

Observed band size: 30 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human TOLLIP. The exact immunogen used to generate this antibody is proprietary information.

Q9H0E2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tollip also known as Toll-interacting protein acts as an important regulator in the immune response. This protein weighs approximately 30 kDa and is found expressed in several tissues especially in the lung liver and spleen. Tollip binds to various ubiquitinated proteins and plays a role in the endocytosis process assisting in protein trafficking. It functions by modulating the signaling pathways that involve innate immunity primarily by interacting with Toll-like receptors (TLRs).

Biological function summary

Toll-interacting protein participates in the negative regulation of the immune response preventing overactivation that could lead to inflammation. Tollip forms a complex with TLRs and IL-1 receptor pathway proteins to control inflammatory signaling. Through its action Tollip binds to Tom1 another protein that links Tollip to phosphatidylinositol-3-phosphate. By regulating these pathways Tollip assists in maintaining a balanced immune response.

Pathways

The protein engages in the TLR signaling pathway and the IL-1 receptor pathway. Both pathways are key in initiating immune responses against pathogens. Tollip as part of these pathways directly associates with proteins like IRAK-1 where it modulates the activity and ensures controlled signaling. This regulation prevents excessive or unnecessary activation that can result in immune-related damage.

Tollip links to conditions such as inflammatory bowel disease and sepsis. Both conditions involve significant immune dysregulation. In these disease states Tollip interacts with proteins like MyD88 affecting the response to pathogens and inflammatory stimuli. Malfunction or altered expression of Tollip can worsen symptoms or contribute to disease progression due to abnormal immune reactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the signaling pathway of IL-1 and Toll-like receptors (PubMed : 10854325, PubMed : 11751856). Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex (PubMed : 10854325). Inhibits IRAK1 phosphorylation and kinase activity (PubMed : 11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates (PubMed : 25042851). The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed : 25042851). In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed : 15047686). Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed : 26320582).

See full target information TOLLIP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 17:626-637 PubMed35434373

2022

Tollip promotes hepatocellular carcinoma progression via PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Huang,Qiong Yang,Huihong Chen,Zhenggeng Wang,Qi Liu,Shuhua Ai

Journal of the American Heart Association 7: PubMed29887521

2018

Tollip Negatively Regulates Vascular Smooth Muscle Cell-Mediated Neointima Formation by Suppressing Akt-Dependent Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhi,Fu-Han Gong,Wen-Lin Cheng,Kongbo Zhu,Long Chen,Yuyu Yao,Xingzhou Ye,Xue-Yong Zhu,Hongliang Li

American journal of physiology. Heart and circulat 311:H822-36 PubMed27521418

2016

Defining the sham environment for post-myocardial infarction studies in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rugmani Padmanabhan Iyer,Lisandra E de Castro Brás,Presley L Cannon,Yonggang Ma,Kristine Y DeLeon-Pennell,Mira Jung,Elizabeth R Flynn,Jeffrey B Henry,Dustin R Bratton,Jared A White,Linda K Fulton,Andrew W Grady,Merry L Lindsey

Journal of neuroinflammation 8:140 PubMed21999375

2011

LPS preconditioning redirects TLR signaling following stroke: TRIF-IRF3 plays a seminal role in mediating tolerance to ischemic injury.

Applications

Species

Unspecified reactive species

Keri B Vartanian,Susan L Stevens,Brenda J Marsh,Rebecca Williams-Karnesky,Nikola S Lessov,Mary P Stenzel-Poore

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Tollip antibody

Western blot - Anti-Tollip antibody (AB37155)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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