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AB170866

Anti-TOM1 antibody [EPR11988(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal TOM1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Target of Myb1 membrane trafficking protein, Target of Myb protein 1, TOM1

2 Images
Western blot - Anti-TOM1 antibody [EPR11988(B)] (AB170866)
  • WB

Lab

Western blot - Anti-TOM1 antibody [EPR11988(B)] (AB170866)

Lanes 1 - 4 : Merged signal (red and green). Green - ab170866 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab170866 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in TOM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab170866 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TOM1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Predicted band size: 54 kDa

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Western blot - Anti-TOM1 antibody [EPR11988(B)] (AB170866)
  • WB

Supplier Data

Western blot - Anti-TOM1 antibody [EPR11988(B)] (AB170866)

All lanes:

Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution

Lane 1:

HepG2 cell lysates at 10 µg

Lane 2:

JAR cell lysates at 10 µg

Lane 3:

HeLa cell lysates at 10 µg

Lane 4:

K562 cell lysates at 10 µg

Predicted band size: 54 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11988(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pathways (PubMed : 14563850, PubMed : 15047686, PubMed : 23023224, PubMed : 25588840, PubMed : 26320582, PubMed : 31371777). Acts as a MYO6/Myosin VI adapter protein that targets MYO6 to endocytic structures (PubMed : 23023224). Together with MYO6, required for autophagosomal delivery of endocytic cargo, the maturation of autophagosomes and their fusion with lysosomes (PubMed : 23023224). MYO6 links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN (PubMed : 31371777). Binds to polyubiquitinated proteins via its GAT domain (PubMed : 14563850). In a complex with TOLLIP, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed : 15047686). The Tom1-Tollip complex may regulate endosomal trafficking by linking polyubiquitinated proteins to clathrin (PubMed : 14563850, PubMed : 15047686). Mediates clathrin recruitment to early endosomes by ZFYVE16 (PubMed : 15657082). Modulates binding of TOLLIP to phosphatidylinositol 3-phosphate (PtdIns(3)P) via binding competition; the association with TOLLIP may favor the release of TOLLIP from endosomal membranes, allowing TOLLIP to commit to cargo trafficking (PubMed : 26320582). Acts as a phosphatidylinositol 5-phosphate (PtdIns(5)P) effector by binding to PtdIns(5)P, thereby regulating endosomal maturation (PubMed : 25588840). PtdIns(5)P-dependent recruitment to signaling endosomes may block endosomal maturation (PubMed : 25588840). Also inhibits Toll-like receptor (TLR) signaling and participates in immune receptor recycling (PubMed : 15047686, PubMed : 26320582).
See full target information Target of Myb protein 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 32:108154 PubMed32937139

2020

Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Schneider,Geoffrey Michael Nelson,Joseph Luke Watson,Jörg Morf,Maximillian Dalglish,Laura Martina Luh,Annika Weber,Anne Bertolotti
View all publications
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Product promise

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