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AB249599

Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TOM1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Target of Myb1 membrane trafficking protein, Target of Myb protein 1, TOM1

1 Images
Western blot - Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (AB249599)
  • WB

Lab

Western blot - Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (AB249599)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170928).

Lanes 1 - 4 : Merged signal (red and green). Green - ab170928 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab170928 was shown to specifically react with in wild-type HAP1 cells as signal was lost in TOM1 knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab170928 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TOM1 antibody [EPR11989(B)] (<a href='/en-us/products/primary-antibodies/tom1-antibody-epr11989b-ab170928'>ab170928</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TOM1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Predicted band size: 54 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11989(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249599 is the carrier-free version of ab170928.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pathways (PubMed : 14563850, PubMed : 15047686, PubMed : 23023224, PubMed : 25588840, PubMed : 26320582, PubMed : 31371777). Acts as a MYO6/Myosin VI adapter protein that targets MYO6 to endocytic structures (PubMed : 23023224). Together with MYO6, required for autophagosomal delivery of endocytic cargo, the maturation of autophagosomes and their fusion with lysosomes (PubMed : 23023224). MYO6 links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN (PubMed : 31371777). Binds to polyubiquitinated proteins via its GAT domain (PubMed : 14563850). In a complex with TOLLIP, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed : 15047686). The Tom1-Tollip complex may regulate endosomal trafficking by linking polyubiquitinated proteins to clathrin (PubMed : 14563850, PubMed : 15047686). Mediates clathrin recruitment to early endosomes by ZFYVE16 (PubMed : 15657082). Modulates binding of TOLLIP to phosphatidylinositol 3-phosphate (PtdIns(3)P) via binding competition; the association with TOLLIP may favor the release of TOLLIP from endosomal membranes, allowing TOLLIP to commit to cargo trafficking (PubMed : 26320582). Acts as a phosphatidylinositol 5-phosphate (PtdIns(5)P) effector by binding to PtdIns(5)P, thereby regulating endosomal maturation (PubMed : 25588840). PtdIns(5)P-dependent recruitment to signaling endosomes may block endosomal maturation (PubMed : 25588840). Also inhibits Toll-like receptor (TLR) signaling and participates in immune receptor recycling (PubMed : 15047686, PubMed : 26320582).
See full target information Target of Myb protein 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com