AB96320

Anti-TOM1L2 antibody

Name: Anti-TOM1L2 antibody (ab96320) | Abcam
Brand: Abcam Limited
SKU: AB96320

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(9 Publications)

Rabbit Polyclonal TOM1L2 antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TOM1L2 aa 350-500.

View Alternative Names

TOM1-like protein 2, Target of Myb-like protein 2, TOM1L2

1 Images

Supplier Data

Western blot - Anti-TOM1L2 antibody (AB96320)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-TOM1L2 antibody (ab96320) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TOM1L2 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q6ZVM7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PubMed : 23023224). May also play a role in recruiting clathrin to endosomes (PubMed : 16412388). May regulate growth factor-induced mitogenic signaling (PubMed : 16479011).

See full target information TOM1L2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 58:677-693.e9 PubMed37019113

2023

The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Swapnil Rohidas Shinde,David U Mick,Erika Aoki,Rachel B Rodrigues,Steven P Gygi,Maxence V Nachury

Biology of reproduction 102:863-875 PubMed31901088

2020

Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis†‡.

Applications

Unspecified application

Species

Unspecified reactive species

Przemysław Zakrzewski,Anna Suwińska,Robert Lenartowski,Maria Jolanta Rędowicz,Folma Buss,Marta Lenartowska

Cell reports 19:2088-2101 PubMed28591580

2017

MYO6 Regulates Spatial Organization of Signaling Endosomes Driving AKT Activation and Actin Dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas A Masters,David A Tumbarello,Margarita V Chibalina,Folma Buss

Proceedings of the National Academy of Sciences of 114:1595-1600 PubMed28143933

2017

Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas A Masters,Folma Buss

The Biochemical journal 473:3307-19 PubMed27474411

2016

Loss of cargo binding in the human myosin VI deafness mutant (R1166X) leads to increased actin filament binding.

Applications

Species

Unspecified reactive species

Susan D Arden,David A Tumbarello,Tariq Butt,John Kendrick-Jones,Folma Buss

PLoS pathogens 11:e1005174 PubMed26451915

2015

The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

David A Tumbarello,Paul T Manna,Mark Allen,Mark Bycroft,Susan D Arden,John Kendrick-Jones,Folma Buss

Journal of cell science 128:815-27 PubMed25588840

2015

TOM1 is a PI5P effector involved in the regulation of endosomal maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric Boal,Rana Mansour,Marion Gayral,Estelle Saland,Gaëtan Chicanne,Jean-Marie Xuereb,Marlène Marcellin,Odile Burlet-Schiltz,Philippe J Sansonetti,Bernard Payrastre,Hélène Tronchère

Autophagy 11:685-700 PubMed25484089

2014

Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Chuan Shen,Huei-Ying Li,Guang-Chao Chen,Yijuang Chern,Pang-Hsien Tu

Nature cell biology 14:1024-35 PubMed23023224

2012

Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome.

Applications

IF, WB

Species

Unspecified reactive species, Unspecified reactive species

David A Tumbarello,Bennett J Waxse,Susan D Arden,Nicholas A Bright,John Kendrick-Jones,Folma Buss

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TOM1L2 antibody

Western blot - Anti-TOM1L2 antibody (AB96320)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise