Rabbit Polyclonal TOM1L2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human TOM1L2 aa 350-500.
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Publications
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- Biology of reproduction 102:863-8752020Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis†‡.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrzemysław Zakrzewski,Anna Suwińska,Robert Lenartowski,Maria Jolanta Rędowicz,Folma Buss,Marta LenartowskaPubMed 31901088
- Cell reports 19:2088-21012017MYO6 Regulates Spatial Organization of Signaling Endosomes Driving AKT Activation and Actin Dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas A Masters,David A Tumbarello,Margarita V Chibalina,Folma BussPubMed 28591580
- Proceedings of the National Academy of Sciences of 114:1595-16002017Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas A Masters et. al.PubMed 28143933
- The Biochemical journal 473:3307-192016Loss of cargo binding in the human myosin VI deafness mutant (R1166X) leads to increased actin filament binding.Applications:WBReactive species:Unspecified reactive speciesSusan D Arden,David A Tumbarello,Tariq Butt,John Kendrick-Jones,Folma BussPubMed 27474411
- PLoS pathogens 11:e10051742015The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid A Tumbarello et. al.PubMed 26451915
- Journal of cell science 128:815-272015TOM1 is a PI5P effector involved in the regulation of endosomal maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrédéric Boal et. al.PubMed 25588840
- Autophagy 11:685-7002014Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Chuan Shen,Huei-Ying Li,Guang-Chao Chen,Yijuang Chern,Pang-Hsien TuPubMed 25484089
- Nature cell biology 14:1024-352012Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome.Applications:IF, WBReactive species:Unspecified reactive speciesDavid A Tumbarello et. al.PubMed 23023224
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TOM1L2 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q6ZVM7
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PubMed:23023224). May also play a role in recruiting clathrin to endosomes (PubMed:16412388). May regulate growth factor-induced mitogenic signaling (PubMed:16479011).
Alternative names
TOM1-like protein 2, Target of Myb-like protein 2, TOM1L2
Recommended products
Rabbit Polyclonal TOM1L2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human TOM1L2 aa 350-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TOM1L2 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q6ZVM7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunoprecipitation - Anti-TOM1L2 antibody (ab96320)
TOM1L2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5μg of Rabbit polyclonal to TOM1L2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96320.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697)
>Band: 56kDa; TOM1L2
All lanes: Immunoprecipitation - Anti-TOM1L2 antibody (ab96320)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Exposure time: 20min
Western blot - Anti-TOM1L2 antibody (ab96320)
7.5% SDS PAGE
All lanes: Western blot - Anti-TOM1L2 antibody (ab96320) at 1/1000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: NT2D1 whole cell lysate at 30 µg
Predicted band size: 56 kDa
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