Rabbit Recombinant Monoclonal TOMM20 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Images
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--flow-cytometry-intracellular-img68625.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--immunocytochemistry-immunofluorescence-img1864.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112151.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130615.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)")
Publications
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- Cell death discovery 7:1872021Deubiquitinating enzyme USP30 negatively regulates mitophagy and accelerates myocardial cell senescence through antagonism of Parkin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Pan,Yaowen Wang,Xinyu Bai,Yuehui Yin,Limeng Dai,Hong Zhou,Qin Wu,Yan WangPubMed 34290230
- The Journal of neuroscience : the official journal of the Society for Neuroscience 41:3731-37462021REST Protects Dopaminergic Neurons from Mitochondrial and α-Synuclein Oligomer Pathology in an Alpha Synuclein Overexpressing BAC-Transgenic Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrent J Ryan,Nora Bengoa-Vergniory,Matthew Williamson,Ecem Kirkiz,Rosalind Roberts,Gabriele Corda,Maximilian Sloan,Saba Saqlain,Marta Cherubini,Josse Poppinga,Helle Bogtofte,Milena Cioroch,Svenja Hester,Richard Wade-MartinsPubMed 33563726
- Nature medicine 22:146-532016Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Expected | Predicted | Predicted | Predicted |
| Rat | Expected | Predicted | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
7 products for Alternative Version
Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016
Recommended products
Rabbit Recombinant Monoclonal TOMM20 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR15581-39
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab220822 is the carrier-free version of Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--flow-cytometry-intracellular-img68625.jpg "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)")
Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
Intracellular Flow Cytometry analysis of TOMM20 expression in NCI-H2228 cells using Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734 at 1/40 dilution (red) and a rabbit IgG as negative control (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734).
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--immunocytochemistry-immunofluorescence-img1864.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TOMM20 (green) with Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112151.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue, labeling TOMM20 with Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-bsa-and-azide-free-ab220822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130615.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue tissue, labeling TOMM20 with Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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