Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)

This data was developed using ab186734 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded human kidney labelling TOMM20 with ab186734 at a concentration of 0.01 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. The heat-mediated antigen retrieval was conducted with DISCOVERY cell conditioning solution (CC1) at 100 °C, pH 8.5 for 32 mins. ab186734 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker was incubated for 16 mins, at 37 °C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue, labeling TOMM20 with ab186734 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186734).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)

Intracellular Flow Cytometry analysis of TOMM20 expression in NCI-H2228 cells using ab186734 at 1/40 dilution (red) and a rabbit IgG as negative control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186734).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TOMM20 (green) with ab186734 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186734).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (AB220822)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue tissue, labeling TOMM20 with ab186734 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186734).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.