Anti-TOMM20 antibody [EPR15581-39] ab186734 is a rabbit monoclonal antibody that is used in TOMM20 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR15581-39 is cited in over 120 publications
- One antibody for all your TOMM20 staining, use in TOMM20 western blotting, IHC, immunofluorescence and flow cytometry
Images
![Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--western-blot-img82051.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunocytochemistry-immunofluorescence-img25123.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110056.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)")
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--flow-cytometry-intracellular-img68625.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90404.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)")
Publications
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- Scientific reports 13:201262023USP18 enhances dengue virus replication by regulating mitochondrial DNA release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJenn-Haung Lai,De-Wei Wu,Chien-Hsiang Wu,Li-Feng Hung,Chuan-Yueh Huang,Shuk-Man Ka,Ann Chen,Ling-Jun HoPubMed 37978268
- PLoS genetics 19:e10110312023MIWI N-terminal RG motif promotes efficient pachytene piRNA production and spermatogenesis independent of LINE1 transposon silencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Wei,Jiongjie Jing,Xiaoyuan Yan,Jeffrey M Mann,Ruirong Geng,Huirong Xie,Elena Y Demireva,Rex A Hess,Deqiang Ding,Chen ChenPubMed 37956204
- Scientific reports 13:144312023Enhanced presynaptic mitochondrial energy production is required for memory formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErica L Underwood,John B Redell,Kimberly N Hood,Mark E Maynard,Michael Hylin,M Neal Waxham,Jing Zhao,Anthony N Moore,Pramod K DashPubMed 37660191
- Stem cell research & therapy 14:2022023Optimization of differential filtration-based mitochondrial isolation for mitochondrial transplant to cerebral organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid F Bodenstein,Pavel Powlowski,Kassandra A Zachos,Dana El Soufi El Sabbagh,Hyunjin Jeong,Liliana Attisano,Landon Edgar,Douglas C Wallace,Ana Cristina AndreazzaPubMed 37580812
- International journal of molecular sciences 24:2023Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSébastien Meurant,Lorris Mauclet,Marc Dieu,Thierry Arnould,Sven Eyckerman,Patricia RenardPubMed 37298552
- Molecular metabolism 72:1017272023Twenty-four hour rhythmicity in mitochondrial network connectivity and mitochondrial respiration; a study in human skeletal muscle biopsies of young lean and older individuals with obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Gemmink,Sabine Daemen,Jakob Wefers,Jan Hansen,Dirk van Moorsel,Puji Astuti,Johanna A Jorgensen,Esther Kornips,Gert Schaart,Joris Hoeks,Patrick Schrauwen,Matthijs K C HesselinkPubMed 37062525
- PLoS genetics 19:e10106562023The RNA binding protein DND1 is elevated in a subpopulation of pro-spermatogonia and targets chromatin modifiers and translational machinery during late gestation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictor A Ruthig,Talia Hatkevich,Josiah Hardy,Matthew B Friedersdorf,Chloé Mayère,Serge Nef,Jack D Keene,Blanche CapelPubMed 36857387
- PLoS pathogens 19:e10111702023Antagonism of ALAS1 by the Measles Virus V protein contributes to degradation of the mitochondrial network and promotes interferon response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPierre Khalfi,Rodolphe Suspène,Kyle A Raymond,Vincent Caval,Grégory Caignard,Noémie Berry,Valérie Thiers,Chantal Combredet,Claude Rufie,Stéphane Rigaud,Amine Ghozlane,Stevenn Volant,Anastassia V Komarova,Frédéric Tangy,Jean-Pierre VartanianPubMed 36802406
- Nature cell biology 25:467-4802023Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElias Adriaenssens,Bob Asselbergh,Pablo Rivera-Mejías,Sven Bervoets,Leen Vendredy,Vicky De Winter,Katrien Spaas,Riet de Rycke,Gert van Isterdael,Francis Impens,Thomas Langer,Vincent TimmermanPubMed 36690850
- Nature communications 14:1732023Effect of β2-agonist treatment on insulin-stimulated peripheral glucose disposal in healthy men in a randomised placebo-controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSten M M van Beek,Yvonne M H Bruls,Froukje Vanweert,Ciarán E Fealy,Niels J Connell,Gert Schaart,Esther Moonen-Kornips,Johanna A Jörgensen,Frédéric M Vaz,Ellen T H C Smeets,Peter J Joris,Anne Gemmink,Riekelt H Houtkooper,Matthijs K C Hesselink,Tore Bengtsson,Bas Havekes,Patrick Schrauwen,Joris HoeksPubMed 36635304
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Expected | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/50 - 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 - 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
12 products for Alternative Product
4 products for Alternative Version
Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016
Recommended products
Anti-TOMM20 antibody [EPR15581-39] ab186734 is a rabbit monoclonal antibody that is used in TOMM20 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR15581-39 is cited in over 120 publications
- One antibody for all your TOMM20 staining, use in TOMM20 western blotting, IHC, immunofluorescence and flow cytometry
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR15581-39
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--western-blot-img82051.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/5000 dilution
All lanes: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunocytochemistry-immunofluorescence-img25123.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling TOMM20 with ab186734 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)1/100 was used as counterstain antibody.
Confocal image showing cytoplasmic staining in HeLa cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110056.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Granular cytoplasmic staining on human cervical cancer.
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--flow-cytometry-intracellular-img68625.jpg "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Intracellular Flow Cytometry analysis of TOMM20 expression in NCI-H2228 (Human lung cancer cell line) cells using ab186734 at 1/40 dilution (pink) and a rabbit IgG as negative control (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90404.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Granular cytoplasmic staining on mouse colon.
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunocytochemistry-immunofluorescence-img71596.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
AltMiD515' expression induces mitochondrial fission.
Confocal microscopy of mock-transfected cells, cells transfected with altMiD51WT, altMiD51LYR→AAA or Drp1K38A immunostained with anti-TOM20 (red channel) and anti-Flag (green channel) monoclonal antibodies. In each image, boxed areas are shown at higher magnification in the bottom right corner.
TOMM20 is detected using ab186734 at 1/500 dilution.
From Figure 12c of Samandi et al.
Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860' PMID: 29083303
Reproduced underthe Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunocytochemistry-immunofluorescence-img1864.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186734 at 1/250 dilution.
Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42638.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Immunohistochemical analysis of paraffin-embedded rat testis tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Granular cytoplasmic staining on rat testis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112151.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue, labeling TOMM20 with ab186734 at 1/100 dilution.
Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130615.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186734 at 1/100 dilution.
Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--western-blot-img82072.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/20000 dilution
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunocytochemistry-immunofluorescence-img71596.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunocytochemistry-immunofluorescence-img1864.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42638.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112151.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130615.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
![Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-mitochondrial-marker-ab186734--western-blot-img82072.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)")
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