Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunohistochemical analysis of formalin fixed paraffin embedded human kidney labelling TOMM20 with ab186734 at a concentration of 0.01 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. The heat-mediated antigen retrieval was conducted with DISCOVERY cell conditioning solution (CC1) at 100 °C, pH 8.5 for 32 mins. ab186734 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker was incubated for 16 mins, at 37 °C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling TOMM20 with ab186734 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/100 was used as counterstain antibody.

Confocal image showing cytoplasmic staining in HeLa cells.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on human cervical cancer.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue, labeling TOMM20 with ab186734 at 1/100 dilution.

Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Intracellular Flow Cytometry analysis of TOMM20 expression in NCI-H2228 (Human lung cancer cell line) cells using ab186734 at 1/40 dilution (pink) and a rabbit IgG as negative control (green).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186734 at 1/250 dilution.

Alexa Fluor^®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on mouse colon.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunohistochemical analysis of paraffin-embedded rat testis tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on rat testis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186734 at 1/100 dilution.

Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

All lanes:

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/5000 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

false

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

AltMiD51^5' expression induces mitochondrial fission.

Confocal microscopy of mock-transfected cells, cells transfected with altMiD51^WT, altMiD51^LYR→AAA or Drp1^K38A immunostained with anti-TOM20 (red channel) and anti-Flag (green channel) monoclonal antibodies. In each image, boxed areas are shown at higher magnification in the bottom right corner.

TOMM20 is detected using ab186734 at 1/500 dilution.

From Figure 12c of Samandi et al.

Samandi et al eLife. 2017; 6 : e27860. Published online 2017 Oct 30. doi : 10.7554/eLife.27860' PMID : 29083303

Reproduced under the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860

• WB

Supplier Data

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (AB186734)

All lanes:

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/20000 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

false