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Mouse Recombinant Monoclonal TOMM20 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

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Images

Western blot - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (AB283317), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (AB283317), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (AB283317), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (AB283317), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (AB283317), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrFlow Cyt (Intra)ICC/IFIHC-P
Human
Tested
Expected
Not recommended
Not recommended
Tested
Mouse
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species

Rat

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

5 products for Alternative Product

3 products for Alternative Version

Target data

Function

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.

Alternative names

Recommended products

Mouse Recombinant Monoclonal TOMM20 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR15581-39

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mitochondrial Marker.

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317), expandable thumbnail

    Western blot - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317)

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: Lane 1: 70 seconds; Lane 2-3: 59 seconds

    Lane 2-3 of this blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 3: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 16 kDa

    Observed band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins. Counterstained with Hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat kidney. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised Mouse small intestine labeling TOMM20 with ab283317 at 1/500 (2.16 ug/ml) dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on mouse small intestine. Nuclear counterstain: DAPI.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] – Mouse IgG1 (Chimeric) (ab283317)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised rat small intestine labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on rat small intestine. Nuclear counterstain: DAPI.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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