Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB283339)

This data was developed using ab283317 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. Counterstained with Hematoxylin.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB283339)

This data was developed using ab283317 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised rat small intestine labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution. Positive staining on rat small intestine. Nuclear counterstain : DAPI.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control : ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB283339)

This data was developed using ab283317 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB283339)

This data was developed using ab283317 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat kidney. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB283339)

This data was developed using ab283317 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised mouse small intestine labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution. Positive staining on mouse small intestine. Nuclear counterstain : DAPI.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control : ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488)

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• WB

Supplier Data

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB283339)

This data was developed using ab283317 the same antibody clone in a different buffer formulation.

Blocking and diluting buffer : 5% NFDM/TBST

Exposure time : Lane 1 : 70 seconds; Lane 2-3 : 59 seconds

Lane 2-3 of this blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker - Mouse IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/tomm20-antibody-epr15581-39-mitochondrial-marker-mouse-igg1-chimeric-ab283317'>ab283317</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 3:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

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