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Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670) is a rat monoclonal antibody that is used to detect TOMM20 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (AB289670), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (AB289670), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (AB289670), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (AB289670), expandable thumbnail
  • Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (AB289670), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Tested
Expected
Tested
Mouse
Expected
Expected
Not recommended
Tested
Expected
Rat
Expected
Expected
Not recommended
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

This antibody does not react with mouse and rat species in IHC.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

This antibody does not react with mouse and rat species in IHC.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

This antibody does not react with mouse and rat species in IHC.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species
Rat
Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.

Alternative names

KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20

Recommended products

Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670) is a rat monoclonal antibody that is used to detect TOMM20 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15581-39
Purification technique
Ion exchange chromatography
Specificity

This antibody does not react with mouse and rat species in IHC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670) is a rat recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of TOMM20?


Anti-TOMM20 [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670) specifically detects a band for TOMM20 (UniProt: Q15388) at a molecular weight of 16kDa.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR15581-39] also available for your convenience:
Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab205486, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab205487, HRP - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab205796, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab214409, Carrier free - Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free ab220822, Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) ab283317, Carrier free - Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free ab283339, ab289670, Carrier free - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free ab289676, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab309083, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) ab309166, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab309167



What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling TOMM20 with ab289670 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab289670 for 30 mins at room temperature and followed by rabbit specific IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling TOMM20 with ab289670 at 1/100 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubuln is labeled using Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labelling TOMM20 with ab289670 at 1/50 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on mouse small intestine. The nuclear conterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling TOMM20 with ab289670 at 1/1000 dilution (Red) compared with a rat monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670), expandable thumbnail

    Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670) at 1/1000 dilution

    All lanes: HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution

    Predicted band size: 16 kDa

    Observed band size: 16 kDa

  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat small intestine tissue labelling TOMM20 with ab289670 at 1/50 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on rat small intestine. The nuclear conterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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