Rat Recombinant Monoclonal TOMM20 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176133.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunocytochemistry-immunofluorescence-img168701.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)")
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunohistochemistry-frozen-sections-img177987.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)")
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--flow-cytometry-intracellular-img169439.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)")
![Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--western-blot-img170945.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Tested |
| Mouse | Expected | Expected | Not recommended | Tested | Expected |
| Rat | Expected | Expected | Not recommended | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This antibody does not react with mouse and rat species in IHC. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody does not react with mouse and rat species in IHC. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes This antibody does not react with mouse and rat species in IHC. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20
Recommended products
Rat Recombinant Monoclonal TOMM20 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15581-39
- Purification technique
- Ion exchange chromatography
- Specificity
This antibody does not react with mouse and rat species in IHC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab289676 is the carrier-free version of Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670.
This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker ab186734). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176133.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)
This data was developed using Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling TOMM20 with Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 for 30 mins at room temperature and followed by rabbit specific IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunocytochemistry-immunofluorescence-img168701.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)
This data was developed using Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling TOMM20 with Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 at 1/100 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubuln is labeled using Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) secondary antibody at 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunohistochemistry-frozen-sections-img177987.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)
This data was developed using Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labelling TOMM20 with Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 at 1/50 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on mouse small intestine. The nuclear conterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--flow-cytometry-intracellular-img169439.jpg "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)")
Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)
This data was developed using Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling TOMM20 with Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 at 1/1000 dilution (Red) compared with a rat monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--western-blot-img170945.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)")
Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)
This data was developed using Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670) at 1/1000 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunohistochemistry-frozen-sections-img174113.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)
This data was developed using Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat small intestine tissue labelling TOMM20 with Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) ab289670 at 1/50 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on rat small intestine. The nuclear conterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-39-rat-igg2a-chimeric-bsa-and-azide-free-ab289676--immunohistochemistry-frozen-sections-img174113.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289676)")
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