Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)

This data was developed using ab289670 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling TOMM20 with ab289670 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab289670 for 30 mins at room temperature and followed by rabbit specific IgG antibody (ab102248) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)

This data was developed using ab289670 the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling TOMM20 with ab289670 at 1/100 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor^® 488 (ab150157) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubuln is labeled using ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (Red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rat IgG H&L (Alexa Fluor^® 488 (ab150157) secondary antibody at 1/1000 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)

This data was developed using ab289670 the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling TOMM20 with ab289670 at 1/1000 dilution (Red) compared with a rat monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)

This data was developed using ab289670 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labelling TOMM20 with ab289670 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on mouse small intestine. The nuclear conterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)

This data was developed using ab289670 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat small intestine tissue labelling TOMM20 with ab289670 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on rat small intestine. The nuclear conterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• WB

Supplier Data

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) - BSA and Azide free (AB289676)

This data was developed using ab289670 the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/tomm20-antibody-epr15581-39-mitochondrial-marker-rat-igg2a-chimeric-ab289670'>ab289670</a>) at 1/1000 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

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