Anti-TOMM20 [EPR15581-54] antibody (ab232589) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect TOMM20 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.
- BSA,?sodium azide, and glycerol-free?for consistent conjugation with fluorochromes, enzymes and more
Images
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-frozen-sections-img38303.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunocytochemistry-immunofluorescence-img45876.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120650.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589)")
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--flow-cytometry-intracellular-img74322.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589)")
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-frozen-sections-img42027.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (AB232589)")
Publications
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- Nature immunology 24:2135-21492023Harnessing CD3 diversity to optimize CAR T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRubí M-H Velasco Cárdenas,Simon M Brandl,Ana Valeria Meléndez,Alexandra Emilia Schlaak,Annabelle Buschky,Timo Peters,Fabian Beier,Bryan Serrels,Sanaz Taromi,Katrin Raute,Simon Hauri,Matthias Gstaiger,Silke Lassmann,Johannes B Huppa,Melanie Boerries,Geoffroy Andrieux,Bertram Bengsch,Wolfgang W Schamel,Susana MinguetPubMed 37932456
- Nature 620:374-3802023cGAS-STING drives ageing-related inflammation and neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammet F Gulen,Natasha Samson,Alexander Keller,Marius Schwabenland,Chong Liu,Selene Glück,Vivek V Thacker,Lucie Favre,Bastien Mangeat,Lona J Kroese,Paul Krimpenfort,Marco Prinz,Andrea AblasserPubMed 37532932
- Nature 615:499-5062023Fumarate induces vesicular release of mtDNA to drive innate immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincent Zecchini,Vincent Paupe,Irene Herranz-Montoya,Joëlle Janssen,Inge M N Wortel,Jordan L Morris,Ashley Ferguson,Suvagata Roy Chowdury,Marc Segarra-Mondejar,Ana S H Costa,Gonçalo C Pereira,Laura Tronci,Timothy Young,Efterpi Nikitopoulou,Ming Yang,Dóra Bihary,Federico Caicci,Shun Nagashima,Alyson Speed,Kalliopi Bokea,Zara Baig,Shamith Samarajiwa,Maxine Tran,Thomas Mitchell,Mark Johnson,Julien Prudent,Christian FrezzaPubMed 36890229
- Molecular metabolism 64:1015672022Dual specificity phosphatase 1 attenuates inflammation-induced cardiomyopathy by improving mitophagy and mitochondrial metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Tan,Yue Zhang,Jing He,Feng Wu,Di Wu,Nengxian Shi,Weifeng Liu,Ziying Li,Wenqian Liu,Hao Zhou,Wenting ChenPubMed 35944900
- Frontiers in physiology 12:7286832021Myofibril and Mitochondrial Area Changes in Type I and II Fibers Following 10 Weeks of Resistance Training in Previously Untrained Men.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBradley A Ruple,Joshua S Godwin,Paulo H C Mesquita,Shelby C Osburn,Casey L Sexton,Morgan A Smith,Jeremy C Ogletree,Michael D Goodlett,Joseph L Edison,Arny A Ferrando,Andrew D Fruge,Andreas N Kavazis,Kaelin C Young,Michael D RobertsPubMed 34630147
- Nucleic acids research 49:5230-52482021DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPedro Silva-Pinheiro,Carlos Pardo-Hernández,Aurelio Reyes,Lisa Tilokani,Anup Mishra,Raffaele Cerutti,Shuaifeng Li,Dieu-Hien Rozsivalova,Sebastian Valenzuela,Sukru A Dogan,Bradley Peter,Patricio Fernández-Silva,Aleksandra Trifunovic,Julien Prudent,Michal Minczuk,Laurence Bindoff,Bertil Macao,Massimo Zeviani,Maria Falkenberg,Carlo ViscomiPubMed 33956154
- PLoS biology 19:e30011662021Neural stem cells traffic functional mitochondria via extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Peruzzotti-Jametti,Joshua D Bernstock,Cory M Willis,Giulia Manferrari,Rebecca Rogall,Erika Fernandez-Vizarra,James C Williamson,Alice Braga,Aletta van den Bosch,Tommaso Leonardi,Grzegorz Krzak,Ágnes Kittel,Cristiane Benincá,Nunzio Vicario,Sisareuth Tan,Carlos Bastos,Iacopo Bicci,Nunzio Iraci,Jayden A Smith,Ben Peacock,Karin H Muller,Paul J Lehner,Edit Iren Buzas,Nuno Faria,Massimo Zeviani,Christian Frezza,Alain Brisson,Nicholas J Matheson,Carlo Viscomi,Stefano PluchinoPubMed 33826607
- Frontiers in physiology 11:5974292021Matrine Protects Cardiomyocytes Against Hyperglycemic Stress by Promoting Mitofusin 2-Induced Mitochondrial Fusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTong Xiao,Jie Huang,Yuan Liu,Yujie Zhao,Manman WeiPubMed 33613300
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested | Tested |
| Mouse | Expected | Predicted | Predicted | Predicted | Predicted |
| Rat | Expected | Expected | Tested | Expected | Expected |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
4 products for Alternative Product
Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20
Recommended products
Anti-TOMM20 [EPR15581-54] antibody (ab232589) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect TOMM20 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.
- BSA,?sodium azide, and glycerol-free?for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15581-54
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232589 is the carrier-free version of Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-frozen-sections-img38303.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Immunohistochemistry (Frozen sections) analysis of rat small intestine tissue sections labeling TOMM20 with Purified Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 at 1/50 (1.9 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunocytochemistry-immunofluorescence-img45876.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 staining TOMM20 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120650.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling TOMM20 with Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--flow-cytometry-intracellular-img74322.jpg "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Intracellular Flow Cytometry analysis of TOMM20 expression in paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells cells using Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 at 1/90 dilution (red) and a rabbit IgG as negative control (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-frozen-sections-img42027.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Immunohistochemistry (Frozen sections) analysis of mouse small intestine tissue sections labeling TOMM20 with Purified Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 at 1/50 (1.9 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-frozen-sections-img127819.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Immunohistochemistry (Frozen sections) analysis of mouse cardiac muscle tissue sections labeling TOMM20 with Purified Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 at 1/50 (1.9 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunocytochemistry-immunofluorescence-img16777.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79354.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-frozen-sections-img127819.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunocytochemistry-immunofluorescence-img16777.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-bsa-and-azide-free-ab232589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79354.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)")
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