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Rabbit Recombinant Monoclonal TOMM20 antibody. Mitochondrion marker. Suitable for WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 161 publications.


Images

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail
  • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail

Publications

  • Cell death discovery 9:4562023
    Pharmacological inhibition of MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1) induces ferroptosis in vascular smooth muscle cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Binjie Yan,Darrell Belke,Yu Gui,Yong-Xiang Chen,Zhi-Sheng Jiang,Xi-Long Zheng
    PubMed 38097554
  • Gut microbes 15:22810112023
    Butyrate reduces adherent-invasive -evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Samira A Hamed,Armaan Mohan,Saranya Navaneetha Krishnan,Arthur Wang,Marija Drikic,Nicole L Prince,Ian A Lewis,Jane Shearer,Åsa V Keita,Johan D Söderholm,Timothy E Shutt,Derek M McKay
    PubMed 38078655

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Expected
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Tested
Rat
Tested
Expected
Tested
Expected
Expected

Tested
Tested

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species

Rat

Dilution info

1/50

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/90

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

    10 products for Alternative Version

      Target data

      Function

      Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.

      Alternative names

      Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016

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      Rabbit Recombinant Monoclonal TOMM20 antibody. Mitochondrion marker. Suitable for WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 161 publications.

      Alternative names

      Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR15581-54

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      16 product images

      • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/10000 dilution

        Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

        Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

        Lane 3: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

        Predicted band size: 16 kDa

        Observed band size: 16 kDa

      • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Immunohistochemistry (Frozen sections) analysis of rat small intestine tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

      • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        ab186735 staining TOMM20 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

        Control: PBS only

      • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/2000 dilution

        Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 10 µg

        Lane 2: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

        Predicted band size: 16 kDa

        Observed band size: 16 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

        Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

      • Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Intracellular Flow Cytometry analysis of TOMM20 expression in paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells cells using ab186735 at 1/90 dilution (red) and a rabbit IgG as negative control (green).

      • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Immunohistochemistry (Frozen sections) analysis of mouse small intestine tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

      • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Immunohistochemistry (Frozen sections) analysis of mouse cardiac muscle tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

      • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized human non-small cell lung carcinoma cells labeleing TOMM20 using ab186735 at 1/400 dilution for 3 hours at room temperature. A Goat anti-Rabbit IgG (H&L) AlexaFluor® was used as secondary antibody at 1/1000 dilution.

      • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186735 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

        Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

      • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

        In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/1000 dilution.

        All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (Anti-Slc25a1 antibody [EPR29193-92] ab318201) at 1/1000 dilution

        Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg

        Lane 2: HeLa non-mitochondrial fraction at 20 µg

        Lane 3: HeLa mitochondrial fraction at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Observed band size: 34 kDa, 36 kDa, 15 kDa

        Exposure time: 15s

      • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Low expression: lung, intestine (PMID: 20861021).

        The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20861021).

        In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

        In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/2000 dilution.

        All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (Anti-MARC1 antibody [EPR28272-93] ab317262) at 1/1000 dilution

        Lane 1: Human liver mitochondria fraction at 20 µg

        Lane 2: Human liver non-mitochondrial fraction at 20 µg

        Lane 3: Human liver tissue lysate at 20 µg

        Lane 4: Human lung tissue lysate at 20 µg

        Lane 5: Human small intestine tissue lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Observed band size: 36 kDa, 16 kDa

        Exposure time: 3s

      • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Low expression: lung, heart (PMID: 20861021).

        The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20861021).

        In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

        In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/2000 dilution.

        All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (Anti-MARC1 antibody [EPR28272-93] ab317262) at 1/1000 dilution

        Lane 1: Mouse liver mitochondria fraction at 20 µg

        Lane 2: Mouse liver non-mitochondrial fraction at 20 µg

        Lane 3: Mouse liver tissue lysate at 20 µg

        Lane 4: Mouse lung tissue lysate at 20 µg

        Lane 5: Mouse heart tissue lysate at 20 µg

        Lane 6: Rat liver tissue lysate at 20 µg

        Lane 7: Rat heart tissue lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Observed band size: 36 kDa, 16 kDa

        Exposure time: 15s

      • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

        In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/2000 dilution.

        All lanes: Western blot - Anti-ATAD3A antibody [EPR27484-26] (Anti-ATAD3A antibody [EPR27484-26] ab317252) at 1/1000 dilution

        Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) non-mitochondrial fraction at 20 µg

        Lane 2: HeLa mitochondria fraction at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Observed band size: 66 kDa, 36 kDa, 16 kDa

        Exposure time: 180s

      • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail

        Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

        In Western blot, Anti-TOMM20 (ab186735) staining at 1/1000 dilution.

        All lanes: Western blot - Anti-NDUFV1 antibody [EPR29179-78] (Anti-NDUFV1 antibody [EPR29179-78] ab321893) at 1/1000 dilution

        Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

        Lane 2: HeLa mitochondrial fraction at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Observed band size: 51 kDa, 36 kDa, 16 kDa

        Exposure time: 26s

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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