Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker is a rabbit recombinant monoclonal antibody that is used to detect TOMM20 in Flow cytometry (Intra), ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 160 publications
Images
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img57067.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)")
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img105870.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)")
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-frozen-sections-img38303.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunocytochemistry-immunofluorescence-img45876.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120650.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)")
Publications
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- Cell death discovery 9:4562023Pharmacological inhibition of MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1) induces ferroptosis in vascular smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBinjie Yan,Darrell Belke,Yu Gui,Yong-Xiang Chen,Zhi-Sheng Jiang,Xi-Long ZhengPubMed 38097554
- Gut microbes 15:22810112023Butyrate reduces adherent-invasive -evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamira A Hamed,Armaan Mohan,Saranya Navaneetha Krishnan,Arthur Wang,Marija Drikic,Nicole L Prince,Ian A Lewis,Jane Shearer,Åsa V Keita,Johan D Söderholm,Timothy E Shutt,Derek M McKayPubMed 38078655
- Cell death & disease 14:7362023FKBP5 activates mitophagy by ablating PPAR-γ to shape a benign remyelination environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingzong Sun,Menghan Qian,Hongliang Li,Lei Wang,Yunjie Zhao,Min Yin,Lili Dai,Hongkun BaoPubMed 37952053
- Cell death discovery 9:4042023NTRK1 knockdown induces mouse cognitive impairment and hippocampal neuronal damage through mitophagy suppression via inactivating the AMPK/ULK1/FUNDC1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Yang,Jue Wu,Shang Li,Shan Wang,Jing Zhang,Yi-Peng Wang,You-Sheng Yan,Hua-Ying Hu,Ming-Fang Xiong,Chao-Bo Bai,Yong-Qing Sun,Wen-Qi Chen,Yang Zeng,Jun-Liang Yuan,Cheng-Hong YinPubMed 37907480
- Journal of translational medicine 21:7562023Mitochondrial modulation with leriglitazone as a potential treatment for Rett syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUliana Musokhranova,Cristina Grau,Cristina Vergara,Laura Rodríguez-Pascau,Clara Xiol,Alba A Castells,Soledad Alcántara,Pilar Rodríguez-Pombo,Pilar Pizcueta,Marc Martinell,Angels García-Cazorla,Alfonso OyarzábalPubMed 37884937
- Cells 12:2023Eye Lens Organoids Made Simple: Characterization of a New Three-Dimensional Organoid Model for Lens Development and Pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthieu Duot,Roselyne Viel,Justine Viet,Catherine Le Goff-Gaillard,Luc Paillard,Salil A Lachke,Carole Gautier-Courteille,David ReboutierPubMed 37887322
- Neuropharmacology 241:1097302023Impact of physical activity on brain oxidative metabolism and intrinsic capacities in young swiss mice fed a high fat diet.Applications:WBReactive species:MouseClémence Viguier,Sébastien Bullich,Marlene Botella,Laure Fasseu,Amélie Alfonso,Khaoula Rekik,Sébastien Gauzin,Bruno P Guiard,Noélie DavezacPubMed 37758019
- Cell death & disease 14:5832023TGR5 supresses cGAS/STING pathway by inhibiting GRP75-mediated endoplasmic reticulum-mitochondrial coupling in diabetic retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Li,Lingpeng Zhu,Meng-Xia Cai,Zi-Li Wang,Miao Zhuang,Cheng-Ye Tan,Tian-Hua Xie,Yong Yao,Ting-Ting WeiPubMed 37658045
- Nature 621:171-1782023Identification of an alternative triglyceride biosynthesis pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGian-Luca McLelland,Marta Lopez-Osias,Cristy R C Verzijl,Brecht D Ellenbroek,Rafaela A Oliveira,Nicolaas J Boon,Marleen Dekker,Lisa G van den Hengel,Rahmen Ali,Hans Janssen,Ji-Ying Song,Paul Krimpenfort,Tim van Zutphen,Johan W Jonker,Thijn R BrummelkampPubMed 37648867
- Theranostics 13:4229-42462023iNOS aggravates pressure overload-induced cardiac dysfunction via activation of the cytosolic-mtDNA-mediated cGAS-STING pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongzheng Guo,Yuehua You,Fei-Fei Shang,Xiaowen Wang,Bi Huang,Boying Zhao,Dingyi Lv,Shenglan Yang,Ming Xie,Lingwen Kong,Dingyuan Du,Suxin Luo,Xin Tian,Yong XiaPubMed 37554263
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | ICC/IF | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Tested |
| Rat | Tested | Expected | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Species Rat | Dilution info 1/50 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/90 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20
Recommended products
Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker is a rabbit recombinant monoclonal antibody that is used to detect TOMM20 in Flow cytometry (Intra), ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 160 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15581-54
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P and WB.
Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) was first used in a scientific publication in 2017 and has been cited over 161 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) has 8 independent reviews from customers.
Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) specifically detects TOMM20 (UniProt ID: Q15388; Molecular weight: 17kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR15581-54 - Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free ab232589.
Antibody clone EPR15581-54 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, HRP, Alexa Fluor® 405, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, APC, PE, Alexa Fluor® 488 (Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab209606, HRP Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab209951, Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab210047, Alexa Fluor® 594 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab210665, Alexa Fluor® 568 Anti-TOMM20 antibody [EPR15581-54] ab210841, Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab221292, APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab225341, PE Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab225342, Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab320072).
TOMM20 is a key protein in neuro research, particularly for its role in mitochondrial function and health. It is part of the translocase of the outer mitochondrial membrane complex, essential for importing proteins into mitochondria. Researchers use TOMM20 to study mitochondrial dynamics, biogenesis and mitophagy, making it vital for understanding cellular energy metabolism and neurodegenerative diseases. Its expression helps in assessing mitochondrial integrity and function in various neurological conditions.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
17 product images
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img57067.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/2000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 10 µg
Lane 2: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img105870.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
All lanes: Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/10000 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Lane 3: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-frozen-sections-img38303.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Immunohistochemistry (Frozen sections) analysis of rat small intestine tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunocytochemistry-immunofluorescence-img45876.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
ab186735 staining TOMM20 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120650.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--flow-cytometry-intracellular-img74322.jpg "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Intracellular Flow Cytometry analysis of TOMM20 expression in paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells cells using ab186735 at 1/90 dilution (red) and a rabbit IgG as negative control (green).
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-frozen-sections-img42027.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Immunohistochemistry (Frozen sections) analysis of mouse small intestine tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-frozen-sections-img127819.jpg "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Immunohistochemistry (Frozen sections) analysis of mouse cardiac muscle tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunocytochemistry-immunofluorescence-img62558.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized human non-small cell lung carcinoma cells labeleing TOMM20 using ab186735 at 1/400 dilution for 3 hours at room temperature. A Goat anti-Rabbit IgG (H&L) AlexaFluor® was used as secondary antibody at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunocytochemistry-immunofluorescence-img16777.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186735 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79354.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img429603.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Low expression: lung, intestine (PMID: 20861021).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20861021).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/2000 dilution.
All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (Anti-MARC1 antibody [EPR28272-93] ab317262) at 1/1000 dilution
Lane 1: Human liver mitochondria fraction at 20 µg with 5% NFDM/TBST
Lane 2: Human liver non-mitochondrial fraction at 20 µg with 5% NFDM/TBST
Lane 3: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Human lung tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Human small intestine tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa, 16 kDa
Exposure time: 3s
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img429604.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Low expression: lung, heart (PMID: 20861021).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20861021).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/2000 dilution.
All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (Anti-MARC1 antibody [EPR28272-93] ab317262) at 1/1000 dilution
Lane 1: Mouse liver mitochondria fraction at 20 µg with 5% NFDM/TBST
Lane 2: Mouse liver non-mitochondrial fraction at 20 µg with 5% NFDM/TBST
Lane 3: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST
Lane 6: Rat liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 7: Rat heart tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa, 16 kDa
Exposure time: 15s
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img430243.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/2000 dilution.
All lanes: Western blot - Anti-ATAD3A antibody [EPR27484-26] (Anti-ATAD3A antibody [EPR27484-26] ab317252) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) non-mitochondrial fraction at 20 µg with NFDM/TBST
Lane 2: HeLa mitochondria fraction at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 66 kDa, 36 kDa, 16 kDa
Exposure time: 180s
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img436164.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-TOMM20 (ab186735) staining at 1/1000 dilution.
All lanes: Western blot - Anti-NDUFV1 antibody [EPR29179-78] (Anti-NDUFV1 antibody [EPR29179-78] ab321893) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg
Lane 2: HeLa mitochondrial fraction at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa, 36 kDa, 16 kDa
Exposure time: 26s
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img433048.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) staining at 1/1000 dilution.
All lanes: Western blot - Anti-Slc25a1 antibody [EPR29193-92] (Anti-Slc25a1 antibody [EPR29193-92] ab318201) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg with NFDM/TBST
Lane 2: HeLa non-mitochondrial fraction at 20 µg with NFDM/TBST
Lane 3: HeLa mitochondrial fraction at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 34 kDa, 36 kDa, 15 kDa
Exposure time: 15s
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img445572.jpg "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
TOMM20 Western blot staining using rabbit Anti-TOMM20 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the higher MW band at around 37 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IFI27 antibody [EPR27275-176] (Anti-IFI27 antibody [EPR27275-176] ab323852) at 1/1000 dilution
Lane 1: Untreated MCF7 (human breast adenocarcinoma epithelial cell) mitochondrial fraction at 60 µg
Lane 2: Untreated MCF7 non-mitochondrial fraction at 60 µg
Lane 3: Untreated MCF7 whole cell lysate at 60 µg
Lane 4: MCF7 treated with 100U/ml IFN alpha 1 for 24 hours, mitochondrial fraction at 60 µg
Lane 5: MCF7 treated with 100U/ml IFN alpha 1 for 24 hours, non-mitochondrial fraction at 60 µg
Lane 6: MCF7 treated with 100U/ml IFN alpha 1 for 24 hours whole cell lysate at 60 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 12 kDa, 36 kDa, 15 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--flow-cytometry-intracellular-img74322.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-frozen-sections-img42027.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-frozen-sections-img127819.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunocytochemistry-immunofluorescence-img62558.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunocytochemistry-immunofluorescence-img16777.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79354.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img429603.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img429604.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img430243.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img436164.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img433048.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
![Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735), expandable thumbnail](https://content.abcam.com/products/images/tomm20-antibody-epr15581-54-mitochondrial-marker-ab186735--western-blot-img445572.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)")
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