Rabbit Polyclonal TOMM22/TOM22 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TOMM22 aa 1-100.
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Publications
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- Structure (London, England : 1993) 29:810-822.e32021Super-resolution imaging reveals cytoskeleton-dependent organelle rearrangement within platelets at intermediate stages of maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeokran Go,Dokyung Jeong,Jinkyoung Chung,Geun-Ho Kim,Jaewoo Song,Eunyoung Moon,Yang Hoon Huh,Doory KimPubMed 34143977
- Scientific reports 11:105112021Super-resolution imaging of platelet-activation process and its quantitative analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinkyoung Chung,Dokyung Jeong,Geun-Ho Kim,Seokran Go,Jaewoo Song,Eunyoung Moon,Yang Hoon Huh,Doory KimPubMed 34006947
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TOMM22 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NS69
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
Target data
Function
Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (PubMed:10982837). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).
Alternative names
TOM22, TOMM22, Mitochondrial import receptor subunit TOM22 homolog, hTom22, 1C9-2, Translocase of outer membrane 22 kDa subunit homolog
Recommended products
Rabbit Polyclonal TOMM22/TOM22 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TOMM22 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TOMM22 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NS69
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
Western blot - Anti-TOMM22/TOM22 antibody (ab246862)
Western blot analysis in human cell line HL-60.
All lanes: Western blot - Anti-TOMM22/TOM22 antibody (ab246862) at 0.4 µg
All lanes: HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody (ab246862)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TOMM22/TOM22 (green) using ab246862 at 4 μg/ml in ICC/IF.
Western blot - Anti-TOMM22/TOM22 antibody (ab246862)
Remaining relative intensity is presented. Loading control: Anti-GAPDH.
All lanes: Western blot - Anti-TOMM22/TOM22 antibody (ab246862) at 0.4 µg/mL
Lane 1: U-2 OS (human bone osteosarcoma epithelial cell line) transfected with control siRNA, whole cell lysate
Lane 2: U-2 OS transfected with target-specific siRNA #1, whole cell lysate
Lane 3: U-2 OS transfected with target-specific siRNA #2, whole cell lysate
Predicted band size: 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)
Paraffin-embedded human duodenum tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)
Paraffin-embedded human fallopian tube tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)
Paraffin-embedded human testis tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)
Paraffin-embedded human pancreas tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.
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