AB246862

Anti-TOMM22/TOM22 antibody

Name: Anti-TOMM22/TOM22 antibody (ab246862) | Abcam
Brand: Abcam Limited
SKU: AB246862
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal TOMM22/TOM22 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TOMM22 aa 1-100.

View Alternative Names

TOM22, TOMM22, Mitochondrial import receptor subunit TOM22 homolog, hTom22, 1C9-2, Translocase of outer membrane 22 kDa subunit homolog

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Paraffin-embedded human pancreas tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody (AB246862)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody (AB246862)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TOMM22/TOM22 (green) using ab246862 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Paraffin-embedded human testis tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Paraffin-embedded human fallopian tube tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Paraffin-embedded human duodenum tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-TOMM22/TOM22 antibody (AB246862)

Remaining relative intensity is presented. Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-TOMM22/TOM22 antibody (ab246862) at 0.4 µg/mL

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) transfected with control siRNA, whole cell lysate

Lane 2:

U-2 OS transfected with target-specific siRNA #1, whole cell lysate

Lane 3:

U-2 OS transfected with target-specific siRNA #2, whole cell lysate

Predicted band size: 15 kDa

false

Unknown

Western blot - Anti-TOMM22/TOM22 antibody (AB246862)

Western blot analysis in human cell line HL-60.

All lanes:

Western blot - Anti-TOMM22/TOM22 antibody (ab246862) at 0.4 µg

All lanes:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TOMM22 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9NS69

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (PubMed : 10982837). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).

See full target information TOMM22

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer informatics 23:11769351241275877 PubMed39238655

2024

Comprehensive Analysis of CCAAT/Enhancer Binding Protein Family in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahong Tan,Daoqi Wang,Wei Dong,Lei Nian,Fen Zhang,Han Zhao,Jie Zhang,Yun Feng

Journal of nanobiotechnology 22:548 PubMed39238028

2024

A nanoscale visual exploration of the pathogenic effects of bacterial extracellular vesicles on host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjae Kang,Min Jeong Kim,Dokyung Jeong,Hyung-Jun Lim,Ga-Eun Go,Uidon Jeong,Eunyoung Moon,Hee-Seok Kweon,Nae-Gyu Kang,Seung Jin Hwang,Sung Hun Youn,Bo Kyoung Hwang,Doory Kim

Structure (London, England : 1993) 29:810-822.e3 PubMed34143977

2021

Super-resolution imaging reveals cytoskeleton-dependent organelle rearrangement within platelets at intermediate stages of maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Seokran Go,Dokyung Jeong,Jinkyoung Chung,Geun-Ho Kim,Jaewoo Song,Eunyoung Moon,Yang Hoon Huh,Doory Kim

Scientific reports 11:10511 PubMed34006947

2021

Super-resolution imaging of platelet-activation process and its quantitative analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinkyoung Chung,Dokyung Jeong,Geun-Ho Kim,Seokran Go,Jaewoo Song,Eunyoung Moon,Yang Hoon Huh,Doory Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TOMM22/TOM22 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody (AB246862)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (AB246862)

Western blot - Anti-TOMM22/TOM22 antibody (AB246862)

Western blot - Anti-TOMM22/TOM22 antibody (AB246862)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise