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Rabbit Polyclonal TOMM40 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Mitochondrial import receptor subunit TOM40 homolog.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (AB272921), expandable thumbnail
  • Western blot - Anti-TOMM40 antibody (AB272921), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (AB272921), expandable thumbnail
  • Western blot - Anti-TOMM40 antibody (AB272921), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Mitochondrial import receptor subunit TOM40 homolog. The exact immunogen used to generate this antibody is proprietary information. Database link O96008

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Expected
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Channel-forming protein essential for import of protein precursors into mitochondria (PubMed:15644312, PubMed:31206022). Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PubMed:31206022).

Alternative names

Recommended products

Rabbit Polyclonal TOMM40 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Mitochondrial import receptor subunit TOM40 homolog.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Mitochondrial import receptor subunit TOM40 homolog. The exact immunogen used to generate this antibody is proprietary information. Database link O96008
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (ab272921), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (ab272921)

    Paraffin-embedded rat colon tissue stained for TOMM40 using ab272921 at 1/2000 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

  • Western blot - Anti-TOMM40 antibody (ab272921), expandable thumbnail

    Western blot - Anti-TOMM40 antibody (ab272921)

    All lanes: Western blot - Anti-TOMM40 antibody (ab272921) at 1/1000 dilution

    Lane 1: HepG2 whole cell extract at 30 µg

    Lane 2: HepG2 mitochondria extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (ab272921), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (ab272921)

    Paraffin-embedded mouse duodenum tissue stained for TOMM40 using ab272921 at 1/2000 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

  • Western blot - Anti-TOMM40 antibody (ab272921), expandable thumbnail

    Western blot - Anti-TOMM40 antibody (ab272921)

    10% SDS-PAGE

    All lanes: Western blot - Anti-TOMM40 antibody (ab272921) at 1/1000 dilution

    Lane 1: HEK-293T whole cell extract at 30 µg

    Lane 2: A431 whole cell extract at 30 µg

    Lane 3: HeLa whole cell extract at 30 µg

    Lane 4: HepG2 whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 38 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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