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AB272921

Anti-TOMM40 antibody

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(3 Publications)

Rabbit Polyclonal TOMM40 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TOMM40.

View Alternative Names

C19orf1, PEREC1, TOM40, TOMM40, Mitochondrial import receptor subunit TOM40 homolog, Protein Haymaker, Translocase of outer membrane 40 kDa subunit homolog, p38.5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (AB272921)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (AB272921)

Paraffin-embedded mouse duodenum tissue stained for TOMM40 using ab272921 at 1/2000 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (AB272921)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody (AB272921)

Paraffin-embedded rat colon tissue stained for TOMM40 using ab272921 at 1/2000 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Western blot - Anti-TOMM40 antibody (AB272921)
  • WB

Supplier Data

Western blot - Anti-TOMM40 antibody (AB272921)

10% SDS-PAGE

All lanes:

Western blot - Anti-TOMM40 antibody (ab272921) at 1/1000 dilution

Lane 1:

HEK-293T whole cell extract at 30 µg

Lane 2:

A431 whole cell extract at 30 µg

Lane 3:

HeLa whole cell extract at 30 µg

Lane 4:

HepG2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38 kDa

false

Western blot - Anti-TOMM40 antibody (AB272921)
  • WB

Supplier Data

Western blot - Anti-TOMM40 antibody (AB272921)

All lanes:

Western blot - Anti-TOMM40 antibody (ab272921) at 1/1000 dilution

Lane 1:

HepG2 whole cell extract at 30 µg

Lane 2:

HepG2 mitochondria extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TOMM40. The exact immunogen used to generate this antibody is proprietary information.

O96008

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Channel-forming protein essential for import of protein precursors into mitochondria (PubMed : 15644312, PubMed : 31206022). Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PubMed : 31206022).
See full target information TOMM40

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 14:e036377 PubMed40028887

2025

Lactate Dehydrogenase A Crotonylation and Mono-Ubiquitination Maintains Vascular Smooth Muscle Cell Growth and Migration and Promotes Neointima Hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Huan Chen,Shan-Hu Cao,Zhi-Yan Ren,Ting Zhang,Han-Mei Jiang,Zhao-Kun Hu,Li-Hua Dong

International journal of molecular sciences 23: PubMed35955572

2022

Sulforaphane Increase Mitochondrial Biogenesis-Related Gene Expression in the Hippocampus and Suppresses Age-Related Cognitive Decline in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sunao Shimizu,Shuya Kasai,Hiromi Yamazaki,Yota Tatara,Junsei Mimura,Máté János Engler,Kunikazu Tanji,Yoshikazu Nikaido,Takuro Inoue,Hiroyuki Suganuma,Koichi Wakabayashi,Ken Itoh

International journal of molecular sciences 23: PubMed35563272

2022

Comparison of BMIPP-SPECT/CT to FDG-PET/CT for Imaging Brown or Browning Fat in a Preclinical Model.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph A Frankl,Yu An,Amber Sherwood,Guiyang Hao,Feng-Yun Huang,Pawan Thapa,Deborah J Clegg,Xiankai Sun,Philipp E Scherer,Orhan K Öz
View all publications

Product promise

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