Rabbit Recombinant Monoclonal TOMM40 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 8 publications.
Images
![Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (AB185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--immunoprecipitation-img68665.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)")
![Western blot - Anti-TOMM40 antibody [EPR6932(2)] (AB185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--western-blot-img57461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)")
![Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (AB185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--flow-cytometry-intracellular-img50892.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (AB185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--immunocytochemistry-immunofluorescence-img2226.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (AB185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100117.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)")
Publications
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- eLife 12:2023Mitochondrial protein import clogging as a mechanism of disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiam P Coyne,Xiaowen Wang,Jiyao Song,Ebbing de Jong,Karin Schneider,Paul T Massa,Frank A Middleton,Thomas Becker,Xin Jie ChenPubMed 37129366
- Nature communications 14:8702023Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarsten Nalbach,Martina Schifferer,Debjani Bhattacharya,Hung Ho-Xuan,Wei Chou Tseng,Luis A Williams,Alexandra Stolz,Stefan F Lichtenthaler,Zvulun Elazar,Christian BehrendsPubMed 36797266
- Molecular medicine (Cambridge, Mass.) 27:72021Inhibition of mitochondrial carrier homolog 2 (MTCH2) suppresses tumor invasion and enhances sensitivity to temozolomide in malignant glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuyun Yuan et. al.PubMed 33509092
- Nature communications 11:45892020Loss of MTX2 causes mandibuloacral dysplasia and links mitochondrial dysfunction to altered nuclear morphology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSahar Elouej et. al.PubMed 32917887
- Nature communications 10:18322019Ceramides bind VDAC2 to trigger mitochondrial apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShashank Dadsena et. al.PubMed 31015432
- Redox biology 25:1011092019HIF-1α protects against oxidative stress by directly targeting mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Sheng Li et. al.PubMed 30686776
- Free radical biology & medicine :2018WITHDRAWN:Mitochondrial targeting of HIF-1α inhibits hypoxia-induced apoptosis independently of its transcriptional activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Sheng Li et. al.PubMed 29704620
- Nature communications 9:3122018Stress-dependent miR-980 regulation of Rbfox1/A2bp1 promotes ribonucleoprotein granule formation and cell survival.Applications:Unspecified applicationReactive species:Drosophila melanogasterMariya M Kucherenko et. al.PubMed 29358748
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 - 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/300 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Channel-forming protein essential for import of protein precursors into mitochondria (PubMed:15644312, PubMed:31206022). Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PubMed:31206022).
Alternative names
C19orf1, PEREC1, TOM40, TOMM40, Mitochondrial import receptor subunit TOM40 homolog, Protein Haymaker, Translocase of outer membrane 40 kDa subunit homolog, p38.5
Recommended products
Rabbit Recombinant Monoclonal TOMM40 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR6932(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--immunoprecipitation-img68665.jpg "Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)")
Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.
All lanes: Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
Predicted band size: 38 kDa
![Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--western-blot-img57461.jpg "Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)")
Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
All lanes: Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543) at 1/10000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: 293T cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--flow-cytometry-intracellular-img50892.jpg "Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)")
Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--immunocytochemistry-immunofluorescence-img2226.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100117.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with ab185543 at 1/500 dilution. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--western-blot-img443044.jpg "Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
TOMM40 western blot using anti-TOMM40 antibody [EPR6932(2)] ab185543. Publication image and figure legend from Yuan, Q., Yang, W., et al., 2021, Mol Med, PubMed 33509092.
ab185543 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab185543 please see the product overview.
High expression of MTCH2 in glioma cells may increase mitochondrial OXPHOs. a, b Western blots and quantifications showing the enriched expression of MTCH2 in glioma cells compared to primary neurons and astrocytes. c qPCR results showing the decreased mRNA level of MTCH2 in A172 cells by siRNA-mediated knockdown (N = 3). d, e Western blots and quantifications showing the decreased protein level of MTCH2 in A172 cells by siRNA-mediated knockdown. Noted that other mitochondrial outer membrane proteins Tom20 and Tom40 were not affected by MTCH2 knockdown. f Immunostaining images showing the mitochondrial localization of MTCH2 in A172 cells. Noted that MTCH2 signals were dramatically decreased by siRNA-mediated knockdown. Scale bar, 10 μm. g Western blots showing the increased mitochondrial respiratory proteins and 4-HNE levels by MTCH2 knockdown in A172 cells
![Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-ab185543--western-blot-img443044.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)")
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