Anti-TOMM40 antibody [EPR6932(2)]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with ab185543 at 1/500 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)

All lanes:

Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

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• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

• IP

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Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)

Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Predicted band size: 38 kDa

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• WB

CiteAb

Western blot - Anti-TOMM40 antibody [EPR6932(2)] (AB185543)

TOMM40 western blot using anti-TOMM40 antibody [EPR6932(2)] ab185543. Publication image and figure legend from Yuan, Q., Yang, W., et al., 2021, Mol Med, PubMed 33509092.

ab185543 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab185543 please see the product overview.

High expression of MTCH2 in glioma cells may increase mitochondrial OXPHOs. a, b Western blots and quantifications showing the enriched expression of MTCH2 in glioma cells compared to primary neurons and astrocytes. c qPCR results showing the decreased mRNA level of MTCH2 in A172 cells by siRNA-mediated knockdown (N = 3). d, e Western blots and quantifications showing the decreased protein level of MTCH2 in A172 cells by siRNA-mediated knockdown. Noted that other mitochondrial outer membrane proteins Tom20 and Tom40 were not affected by MTCH2 knockdown. f Immunostaining images showing the mitochondrial localization of MTCH2 in A172 cells. Noted that MTCH2 signals were dramatically decreased by siRNA-mediated knockdown. Scale bar, 10 μm. g Western blots showing the increased mitochondrial respiratory proteins and 4-HNE levels by MTCH2 knockdown in A172 cells

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