Rabbit Recombinant Monoclonal TOMM40 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--immunoprecipitation-img68665.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964)")
![Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--flow-cytometry-intracellular-img50892.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964)")
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--immunocytochemistry-immunofluorescence-img2226.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100117.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (AB240964)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Channel-forming protein essential for import of protein precursors into mitochondria (PubMed:15644312, PubMed:31206022). Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PubMed:31206022).
Alternative names
C19orf1, PEREC1, TOM40, TOMM40, PEREC1, C19orf1, TOM40, Mitochondrial import receptor subunit TOM40 homolog, Protein Haymaker, Translocase of outer membrane 40 kDa subunit homolog, p38.5
Recommended products
Rabbit Recombinant Monoclonal TOMM40 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR6932(2)
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab240964 is the carrier-free version of Anti-TOMM40 antibody [EPR6932(2)] ab185543.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--immunoprecipitation-img68665.jpg "Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)")
Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. Anti-TOMM40 antibody [EPR6932(2)] ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM40 antibody [EPR6932(2)] ab185543).
All lanes: Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (Anti-TOMM40 antibody [EPR6932(2)] ab185543)
Predicted band size: 38 kDa
![Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--flow-cytometry-intracellular-img50892.jpg "Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)")
Flow Cytometry (Intracellular) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with Anti-TOMM40 antibody [EPR6932(2)] ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM40 antibody [EPR6932(2)] ab185543).
![Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--immunocytochemistry-immunofluorescence-img2226.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)")
Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with Anti-TOMM40 antibody [EPR6932(2)] ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM40 antibody [EPR6932(2)] ab185543).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964), expandable thumbnail](https://content.abcam.com/products/images/tomm40-antibody-epr69322-bsa-and-azide-free-ab240964--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100117.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with Anti-TOMM40 antibody [EPR6932(2)] ab185543 at 1/500 dilution. Counter stain Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TOMM40 antibody [EPR6932(2)] ab185543).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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