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AB121681

Anti-TOP1MT antibody

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(1 Publication)

Rabbit Polyclonal TOP1MT antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TOP1MT aa 1-150.

View Alternative Names

TOP1mt, TOP1MT

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOP1MT antibody (AB121681)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOP1MT antibody (AB121681)

ab121681 at 1/175 dilution staining TOP1MT in Paraffin-embedded Human Colon tissue by Immunohistochemistry

Western blot - Anti-TOP1MT antibody (AB121681)
  • WB

Unknown

Western blot - Anti-TOP1MT antibody (AB121681)

All lanes:

Western blot - Anti-TOP1MT antibody (ab121681) at 1/250 dilution

Lane 1:

RT 4 lysate

Lane 2:

U251 MG lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 70 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TOP1MT aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q969P6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
See full target information TOP1MT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:1127753 PubMed36699464

2023

The impact of decreased expression of SVEP1 on abnormal neovascularization and poor prognosis in patients with intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Chen,Yuchao He,Zhiqiang Han,Wenchen Gong,Xiangdong Tian,Lin Guo,Hua Guo,Tianqiang Song,Lu Chen
View all publications
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Product promise

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