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AB183520

Anti-TOP3B antibody [EP7779] - C-terminal

4

(2 Reviews)

|

(7 Publications)

Rabbit Monoclonal TOP3B antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

TOP3B1, TOP3B, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1

2 Images
Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520)
  • WB

Supplier Data

Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520)

All lanes:

Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 97 kDa

false

Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520)
  • WB

Supplier Data

Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520)

All lanes:

Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 97 kDa

false

  • Carrier free

    Anti-TOP3B antibody [EP7779] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7779

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
See full target information TOP3B
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7524 PubMed37980342

2023

The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav Saha,Shar-Yin Naomi Huang,Xi Yang,Liton Kumar Saha,Yilun Sun,Prashant Khandagale,Lisa M Jenkins,Yves Pommier

Cell reports 40:111067 PubMed35830799

2022

Resolution of R-loops by topoisomerase III-β (TOP3B) in coordination with the DEAD-box helicase DDX5.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav Saha,Xi Yang,Shar-Yin Naomi Huang,Keli Agama,Simone Andrea Baechler,Yilun Sun,Hongliang Zhang,Liton Kumar Saha,Shuaikun Su,Lisa M Jenkins,Weidong Wang,Yves Pommier

International journal of molecular sciences 22: PubMed34884616

2021

Topoisomerase IIIβ Deficiency Induces Neuro-Behavioral Changes and Brain Connectivity Alterations in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Faiz Ur Rahman,You-Rim Kim,Eun-Kyeung Kim,Hae-Rim Kim,Sang-Mi Cho,Chin-Soo Lee,Su Jin Kim,Kimi Araki,Ken-Ichi Yamamura,Mi-Ni Lee,Seul Gi Park,Won-Kee Yoon,Kihoon Lee,Young-Suk Won,Hyoung-Chin Kim,Younghee Lee,Ho-Young Lee,Ki-Hoan Nam

Cell reports 33:108569 PubMed33378676

2020

DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav Saha,Yilun Sun,Shar-Yin Naomi Huang,Simone Andrea Baechler,Lorinc Sandor Pongor,Keli Agama,Ukhyun Jo,Hongliang Zhang,Yuk-Ching Tse-Dinh,Yves Pommier

bioRxiv : the preprint server for biology : PubMed32511318

2020

Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses.

Applications

Unspecified application

Species

Unspecified reactive species

K Reddisiva Prasanth,Minato Hirano,W Samuel Fagg,Eileen T McAnarney,Chao Shan,Xuping Xie,Adam Hage,Colette A Pietzsch,Alexander Bukreyev,Ricardo Rajsbaum,Pei-Yong Shi,Mark T Bedford,Shelton S Bradrick,Vineet Menachery,Mariano A Garcia-Blanco

Bioorganic & medicinal chemistry 28:115352 PubMed32044229

2020

CT2-3, a novel magnolol analogue suppresses NSCLC cells through triggering cell cycle arrest and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Chen,Cheng Tao,Xiaofei Huang,Zide Chen,Li Wang,Xinping Li,Min Ma,Zhengzhi Wu

Nucleic acids research 46:3061-3074 PubMed29471495

2018

Arginine methylation of the C-terminus RGG motif promotes TOP3B topoisomerase activity and stress granule localization.

Applications

Unspecified application

Species

Unspecified reactive species

Lifeng Huang,Zhihao Wang,Nithya Narayanan,Yanzhong Yang
View all publications
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Product promise

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