Rabbit Monoclonal TOP3B antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.
Images
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520), expandable thumbnail](https://content.abcam.com/products/images/top3b-antibody-ep7779-c-terminal-ab183520--western-blot-img125351.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520)")
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520), expandable thumbnail](https://content.abcam.com/products/images/top3b-antibody-ep7779-c-terminal-ab183520--western-blot-img53223.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (AB183520)")
Publications
Filter by
- Nature communications 14:75242023The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSourav Saha,Shar-Yin Naomi Huang,Xi Yang,Liton Kumar Saha,Yilun Sun,Prashant Khandagale,Lisa M Jenkins,Yves PommierPubMed 37980342
- Cell reports 40:1110672022Resolution of R-loops by topoisomerase III-β (TOP3B) in coordination with the DEAD-box helicase DDX5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSourav Saha,Xi Yang,Shar-Yin Naomi Huang,Keli Agama,Simone Andrea Baechler,Yilun Sun,Hongliang Zhang,Liton Kumar Saha,Shuaikun Su,Lisa M Jenkins,Weidong Wang,Yves PommierPubMed 35830799
- International journal of molecular sciences 22:2021Topoisomerase IIIβ Deficiency Induces Neuro-Behavioral Changes and Brain Connectivity Alterations in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFaiz Ur Rahman,You-Rim Kim,Eun-Kyeung Kim,Hae-Rim Kim,Sang-Mi Cho,Chin-Soo Lee,Su Jin Kim,Kimi Araki,Ken-Ichi Yamamura,Mi-Ni Lee,Seul Gi Park,Won-Kee Yoon,Kihoon Lee,Young-Suk Won,Hyoung-Chin Kim,Younghee Lee,Ho-Young Lee,Ki-Hoan NamPubMed 34884616
- Cell reports 33:1085692020DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSourav Saha,Yilun Sun,Shar-Yin Naomi Huang,Simone Andrea Baechler,Lorinc Sandor Pongor,Keli Agama,Ukhyun Jo,Hongliang Zhang,Yuk-Ching Tse-Dinh,Yves PommierPubMed 33378676
- bioRxiv : the preprint server for biology :2020Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Reddisiva Prasanth,Minato Hirano,W Samuel Fagg,Eileen T McAnarney,Chao Shan,Xuping Xie,Adam Hage,Colette A Pietzsch,Alexander Bukreyev,Ricardo Rajsbaum,Pei-Yong Shi,Mark T Bedford,Shelton S Bradrick,Vineet Menachery,Mariano A Garcia-BlancoPubMed 32511318
- Bioorganic & medicinal chemistry 28:1153522020CT2-3, a novel magnolol analogue suppresses NSCLC cells through triggering cell cycle arrest and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Chen et. al.PubMed 32044229
- Nucleic acids research 46:3061-30742018Arginine methylation of the C-terminus RGG motif promotes TOP3B topoisomerase activity and stress granule localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLifeng Huang,Zhihao Wang,Nithya Narayanan,Yanzhong YangPubMed 29471495
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Target data
Function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Alternative names
TOP3B1, TOP3B, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1
Recommended products
Rabbit Monoclonal TOP3B antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP7779
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520), expandable thumbnail](https://content.abcam.com/products/images/top3b-antibody-ep7779-c-terminal-ab183520--western-blot-img125351.jpg "Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)")
Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
All lanes: Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution
Lane 1: K562 cell lysate at 20 µg
Lane 2: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520), expandable thumbnail](https://content.abcam.com/products/images/top3b-antibody-ep7779-c-terminal-ab183520--western-blot-img53223.jpg "Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)")
Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
All lanes: Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: Raw264.7 cell lysate at 10 µg
Lane 3: PC-12 cell lysate at 10 µg
Lane 4: NIH 3T3 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa
Downloads
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