AB200869

Anti-Topoisomerase I antibody [23B11]

Name: Anti-Topoisomerase I antibody [23B11] (ab200869) | Abcam
Brand: Abcam Limited
SKU: AB200869
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(1 Publication)

Mouse Monoclonal Topoisomerase I antibody. Suitable for WB and reacts with Mouse, Rat, Human, Dog samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TOP1 aa 650-750 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DNA topoisomerase 1, DNA topoisomerase I, TOP1

2 Images

Supplier Data

Western blot - Anti-Topoisomerase I antibody [23B11] (AB200869)

PVDF membrane.

All lanes:

Western blot - Anti-Topoisomerase I antibody [23B11] (ab200869) at 0.5 µg/mL

Lane 1:

Whole cell lysate of serum starved HeLa cells at 20000 Cells

Lane 2:

Whole cell lysate of serum starved HepG2 cells at 20000 Cells

Lane 3:

Whole cell lysate of serum starved HEK293 cells at 20000 Cells

Lane 4:

Whole cell lysate of serum starved SH-SY5Y cells at 20000 Cells

Lane 5:

Whole cell lysate of serum starved MDCK cells at 20000 Cells

Lane 6:

Whole cell lysate of serum starved PC12 cells at 20000 Cells

Lane 7:

Whole cell lysate of serum starved CMT 93 cells at 20000 Cells

Lane 8:

Whole cell lysate of serum starved Neuro 2A cells at 20000 Cells

Lane 9:

Whole cell lysate of serum starved 3T3 tumor cells at 20000 Cells

Predicted band size: 91 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-Topoisomerase I antibody [23B11] (AB200869)

The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate

All lanes:

Western blot - Anti-Topoisomerase I antibody [23B11] (ab200869) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 30 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 30 µg

Lane 5:

NTERA-2 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

Lane 6:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 91 kDa

Observed band size: ~90 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

23B11

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Dog, Human

Applications

applications

Immunogen

Synthetic Peptide within Human TOP1 aa 650-750 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P11387

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase I also known as DNA topoisomerase I or Topo I is an important enzyme in the unwinding of the DNA double helix during processes such as replication and transcription. This enzyme has a molecular mass of approximately 91 kDa and functions by creating transient single-strand breaks in the DNA allowing relaxation of supercoils. Topoisomerase I is widely expressed in both proliferating and non-proliferating cells with higher levels seen in tissues with rapid cell division like the gastrointestinal tract and some immune cells.

Biological function summary

Topoisomerase I plays a significant role in the modulation of DNA topology ensuring proper chromosomal functions during cellular proliferation. It serves as a single polypeptide and does not require a companion for its activity unlike other topoisomerases that may function in complexes. The enzyme’s ability to relieve torsional stress in DNA is essential for maintaining genomic stability and facilitating the smooth progression of the replication fork.

Pathways

Topoisomerase I is vital for DNA replication and transcription processes. It works in concert with other proteins such as helicases and ligases to ensure efficient unwinding and rewinding of DNA strands. The enzyme participates in the DNA damage response pathway where it interacts with proteins like PARP1 to coordinate repair processes. Its action is important in both the S phase of the cell cycle where DNA synthesis occurs and in the G0 phase where cells are in a quiescent state.

Topoisomerase I is heavily implicated in oncogenesis with significant associations to colorectal and ovarian cancers. Its heightened activity can lead to genomic instability a hallmark of cancer development. The enzyme also relates to chemotherapeutic resistance where mutations in topoisomerase I lead to reduced drug efficacy. Furthermore inhibitors targeting topoisomerase I such as camptothecin-based drugs exploit its role in cancer aiming to induce DNA damage selectively in rapidly dividing cells. Connections to other proteins like BRCA1 are apparent in these pathways as they both contribute to the DNA repair processes in cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter.

See full target information TOP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:899725 PubMed35774610

2022

A KRAS-Associated Signature for Prognostic, Immune and Chemical Anti-Cancer Drug-Response Prediction in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kangjia Luo,Yanni Song,Zilong Guan,Suwen Ou,Jinhua Ye,Songlin Ran,Hufei Wang,Yangbao Tao,Zijian Gong,Tianyi Ma,Yinghu Jin,Rui Huang,Feng Gao,Shan Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Topoisomerase I antibody [23B11]

Western blot - Anti-Topoisomerase I antibody [23B11] (AB200869)

Western blot - Anti-Topoisomerase I antibody [23B11] (AB200869)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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