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Rabbit Recombinant Monoclonal Topoisomerase I antibody. Suitable for WB, ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (AB131166), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (AB131166), expandable thumbnail
  • OI-RD Scanning - Anti-Topoisomerase I antibody [EPR5376(2)] (AB131166), expandable thumbnail
  • Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (AB131166), expandable thumbnail
  • Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (AB131166), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter.

Alternative names

DNA topoisomerase 1, DNA topoisomerase I, TOP1

Recommended products

Rabbit Recombinant Monoclonal Topoisomerase I antibody. Suitable for WB, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5376(2)
Purification technique
Affinity purification Protein A
Dissociation constant
4.67 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Topoisomerase I also known as DNA topoisomerase I or Topo I is an important enzyme in the unwinding of the DNA double helix during processes such as replication and transcription. This enzyme has a molecular mass of approximately 91 kDa and functions by creating transient single-strand breaks in the DNA allowing relaxation of supercoils. Topoisomerase I is widely expressed in both proliferating and non-proliferating cells with higher levels seen in tissues with rapid cell division like the gastrointestinal tract and some immune cells.

Biological function summary

Topoisomerase I plays a significant role in the modulation of DNA topology ensuring proper chromosomal functions during cellular proliferation. It serves as a single polypeptide and does not require a companion for its activity unlike other topoisomerases that may function in complexes. The enzyme’s ability to relieve torsional stress in DNA is essential for maintaining genomic stability and facilitating the smooth progression of the replication fork.

Pathways

Topoisomerase I is vital for DNA replication and transcription processes. It works in concert with other proteins such as helicases and ligases to ensure efficient unwinding and rewinding of DNA strands. The enzyme participates in the DNA damage response pathway where it interacts with proteins like PARP1 to coordinate repair processes. Its action is important in both the S phase of the cell cycle where DNA synthesis occurs and in the G0 phase where cells are in a quiescent state.

Associated diseases and disorders

Topoisomerase I is heavily implicated in oncogenesis with significant associations to colorectal and ovarian cancers. Its heightened activity can lead to genomic instability a hallmark of cancer development. The enzyme also relates to chemotherapeutic resistance where mutations in topoisomerase I lead to reduced drug efficacy. Furthermore inhibitors targeting topoisomerase I such as camptothecin-based drugs exploit its role in cancer aiming to induce DNA damage selectively in rapidly dividing cells. Connections to other proteins like BRCA1 are apparent in these pathways as they both contribute to the DNA repair processes in cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

    Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Topoisomerase I with purified ab131166 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

    Immunofluorescent analysis of MCF7 cells labelling Topoisomerase I with ab131166 at 1/250 dilution.

  • OI-RD Scanning - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166), expandable thumbnail

    OI-RD Scanning - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166), expandable thumbnail

    Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

    Blocking and diluting buffer: 5%NFDM/TBST.
    We recommend to use 1%SDS Hot lysis prepare method.

    All lanes: Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166) at 1/10000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

    Lane 3: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 4: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Predicted band size: 91 kDa

    Observed band size: 91 kDa

  • Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166), expandable thumbnail

    Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166)

    Blocking buffer: 5%NFDM/TBST.

    All lanes: Western blot - Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166) at 1/1000 dilution

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate, prepared in RIPA lysis method at 20 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate, prepared in 1% SDS HOT lysis method at 20 µg

    Lane 3: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate, prepared in RIPA lysis method at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate, prepared in 1% SDS HOT lysis method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 91 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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