Anti-Topoisomerase II alpha antibody [TOP2A/1361]
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(2 Publications)
Mouse Monoclonal Topoisomerase II alpha antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TOP2A aa 1350-1500.
View Alternative Names
TOP2, TOP2A, DNA topoisomerase 2-alpha
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Topoisomerase II alpha with ab219320 at 1 μg/ml.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Topoisomerase II alpha with ab219320 at 1 μg/ml.
- Protein Array
Unknown
Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
ab219320 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
- WB
Supplier Data
Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
All lanes:
Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (ab219320) at 1 µg/mL
Lane 1:
HepG2 cell lysate
Lane 2:
HeLa cell lysate
Lane 3:
3T3 cell lysate
Predicted band size: 174 kDa
false
Reactivity data
Properties and storage information
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Purification notes
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Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TOP2A is essential for chromosome condensation segregation and relief of DNA torsional stress during mitosis. It does not function in isolation but interacts with other proteins as part of the larger DNA topoisomerase complex. This complex maintains DNA integrity during cellular processes such as transcription and replication. TOP2A's activity ensures that cells can duplicate their genetic material accurately facilitating proper cell division and maintaining genomic stability.
Pathways
TOP2A plays a significant role in the DNA replication and cell cycle regulation pathways. It interacts closely with proteins like CDC2 (cyclin-dependent kinase 1) and is regulated by cyclins which control the timing of cell cycle progression. Additionally TOP2A is involved in the DNA damage response pathway where it collaborates with proteins such as ATM (ataxia-telangiectasia mutated) emphasizing its importance in maintaining DNA structure during replication stress.
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Target data
Publications (2)
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Scientific reports 14:3695 PubMed38355790
2024
Applications
Unspecified application
Species
Unspecified reactive species
Bioengineered 12:12003-12022 PubMed34787052
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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