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AB219320

Anti-Topoisomerase II alpha antibody [TOP2A/1361]

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(2 Publications)

Mouse Monoclonal Topoisomerase II alpha antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TOP2A aa 1350-1500.

View Alternative Names

TOP2, TOP2A, DNA topoisomerase 2-alpha

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Topoisomerase II alpha with ab219320 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Topoisomerase II alpha with ab219320 at 1 μg/ml.

Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
  • Protein Array

Unknown

Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)

ab219320 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)
  • WB

Supplier Data

Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (AB219320)

All lanes:

Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1361] (ab219320) at 1 µg/mL

Lane 1:

HepG2 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

3T3 cell lysate

Predicted band size: 174 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TOP2A/1361

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TOP2A aa 1350-1500. The exact immunogen used to generate this antibody is proprietary information.

P11388

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab219320 was purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II alpha also known as TOP2A is an enzyme that plays a critical role in DNA replication and cell division. It is a homodimeric protein with a mass of around 170 kDa and primarily localizes in the nucleus. TOP2A catalyzes the transient breaking and rejoining of double-stranded DNA necessary for resolving DNA tangles and supercoils during the replication process. TOP2A is highly expressed in proliferating cells due to its role in cell cycle progression making it a significant target in cancer research.
Biological function summary

TOP2A is essential for chromosome condensation segregation and relief of DNA torsional stress during mitosis. It does not function in isolation but interacts with other proteins as part of the larger DNA topoisomerase complex. This complex maintains DNA integrity during cellular processes such as transcription and replication. TOP2A's activity ensures that cells can duplicate their genetic material accurately facilitating proper cell division and maintaining genomic stability.

Pathways

TOP2A plays a significant role in the DNA replication and cell cycle regulation pathways. It interacts closely with proteins like CDC2 (cyclin-dependent kinase 1) and is regulated by cyclins which control the timing of cell cycle progression. Additionally TOP2A is involved in the DNA damage response pathway where it collaborates with proteins such as ATM (ataxia-telangiectasia mutated) emphasizing its importance in maintaining DNA structure during replication stress.

Overexpression of TOP2A is frequently associated with various cancers including breast and prostate cancer. The enzyme's increased activity promotes uncontrolled cell proliferation a hallmark of cancer development. Antitopoisomerase drugs which target this pathway are used in cancer treatments to inhibit TOP2A activity. Additionally mutations or dysregulation of TOP2A-related pathways may also contribute to chemoresistance. Understanding TOP2A interactions and regulation provides insight into its role in cancer and opens avenues for targeted therapeutic interventions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed : 17567603, PubMed : 18790802, PubMed : 22013166, PubMed : 22323612, PubMed : 27754753). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
See full target information TOP2A

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:3695 PubMed38355790

2024

High serum alpha-fetoprotein and positive immunohistochemistry of alpha-fetoprotein are related to poor prognosis of gastric cancer with liver metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuriko Takayama-Isagawa,Kengo Kanetaka,Shinichiro Kobayashi,Akira Yoneda,Shinichiro Ito,Susumu Eguchi

Bioengineered 12:12003-12022 PubMed34787052

2021

Rhophilin rho GTPase binding protein 1-antisense RNA 1 (RHPN1-AS1) promotes ovarian carcinogenesis by sponging microRNA-485-5p and releasing DNA topoisomerase II alpha ().

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhou,Jing Li,Xiaoxin Yang,Yu Song,Haigang Li
View all publications

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