Anti-Topoisomerase II alpha antibody [TOP2A/1362]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (AB219321)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human bladder carcinoma tissue labeling Topoisomerase II alpha with ab219321 at 1 μg/ml.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (AB219321)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue labeling Topoisomerase II alpha with ab219321 at 1 μg/ml.

• ICC

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Immunocytochemistry - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (AB219321)

Immunocytochemistry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Topoisomerase II alpha with ab219321 at 1 μg/ml (Green). Phalloidin (Red) is used to label actin.

• WB

Supplier Data

Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (AB219321)

Western blot analysis of HeLa cell lysate labeling Topoisomerase II alpha with ab219321 at 1 ug/ml.

All lanes:

Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321) at 1 µg/mL

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 174 kDa

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• Protein Array

Unknown

Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (AB219321)

ab219321 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.