AB75765

Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Topoisomerase II alpha phospho S1106 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

TOP2, TOP2A, DNA topoisomerase 2-alpha

2 Images

Unknown

Western blot - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (AB75765)

All lanes:

Western blot - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765) at 1/500 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates, treated with AP at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 174 kDa

Observed band size: 174 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (AB75765)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (AB75765)

ab75765 at 1/100 dilution staining Topoisomerase II alpha in colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Carrier free

Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2722

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II alpha also known as TOP2A is an enzyme that plays a critical role in DNA replication and cell division. It is a homodimeric protein with a mass of around 170 kDa and primarily localizes in the nucleus. TOP2A catalyzes the transient breaking and rejoining of double-stranded DNA necessary for resolving DNA tangles and supercoils during the replication process. TOP2A is highly expressed in proliferating cells due to its role in cell cycle progression making it a significant target in cancer research.

Biological function summary

TOP2A is essential for chromosome condensation segregation and relief of DNA torsional stress during mitosis. It does not function in isolation but interacts with other proteins as part of the larger DNA topoisomerase complex. This complex maintains DNA integrity during cellular processes such as transcription and replication. TOP2A's activity ensures that cells can duplicate their genetic material accurately facilitating proper cell division and maintaining genomic stability.

Pathways

TOP2A plays a significant role in the DNA replication and cell cycle regulation pathways. It interacts closely with proteins like CDC2 (cyclin-dependent kinase 1) and is regulated by cyclins which control the timing of cell cycle progression. Additionally TOP2A is involved in the DNA damage response pathway where it collaborates with proteins such as ATM (ataxia-telangiectasia mutated) emphasizing its importance in maintaining DNA structure during replication stress.

Overexpression of TOP2A is frequently associated with various cancers including breast and prostate cancer. The enzyme's increased activity promotes uncontrolled cell proliferation a hallmark of cancer development. Antitopoisomerase drugs which target this pathway are used in cancer treatments to inhibit TOP2A activity. Additionally mutations or dysregulation of TOP2A-related pathways may also contribute to chemoresistance. Understanding TOP2A interactions and regulation provides insight into its role in cancer and opens avenues for targeted therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed : 17567603, PubMed : 18790802, PubMed : 22013166, PubMed : 22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).

See full target information TOP2A phospho S1106

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 7:40-52 PubMed30267685

2018

A Large-Scale Gene Expression Intensity-Based Similarity Metric for Drug Repositioning.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Tsung Huang,Chiao-Hui Hsieh,Yen-Jen Oyang,Hsuan-Cheng Huang,Hsueh-Fen Juan

Oncotarget 9:29727-29742 PubMed30038716

2018

MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERα and Survivin inhibition and enhances response to temozolomide.

Applications

Unspecified application

Species

Unspecified reactive species

George C Bobustuc,Amin B Kassam,Richard A Rovin,Sheila Jeudy,Joshua S Smith,Beth Isley,Maharaj Singh,Ameya Paranjpe,Kalkunte S Srivenugopal,Santhi D Konduri

Experimental cell research 351:11-23 PubMed28034673

2016

High-content image informatics of the structural nuclear protein NuMA parses trajectories for stem/progenitor cell lineages and oncogenic transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastián L Vega,Er Liu,Varun Arvind,Jared Bushman,Hak-Joon Sung,Matthew L Becker,Sophie Lelièvre,Joachim Kohn,Pierre-Alexandre Vidi,Prabhas V Moghe

Biochimica et biophysica acta 1833:3093-3103 PubMed23999236

2013

DNA damage response (DDR) induced by topoisomerase II poisons requires nuclear function of the small GTPase Rac.

Applications

Species

Human

Friedrich Wartlick,Anita Bopp,Christian Henninger,Gerhard Fritz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722]

Western blot - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (AB75765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (AB75765)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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