Rabbit Recombinant Monoclonal Topoisomerase II alpha phospho T1343 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (AB52853), expandable thumbnail](https://content.abcam.com/products/images/topoisomerase-ii-alpha-phospho-t1343-antibody-ep1054y-ab52853--western-blot-img137828.gif "Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (AB52853)")
![Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (AB52853), expandable thumbnail](https://content.abcam.com/products/images/topoisomerase-ii-alpha-phospho-t1343-antibody-ep1054y-ab52853--immunocytochemistry-immunofluorescence-img59977.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (AB52853)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
Alternative names
TOP2, TOP2A, DNA topoisomerase 2-alpha
Recommended products
Rabbit Recombinant Monoclonal Topoisomerase II alpha phospho T1343 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1054Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Topoisomerase II alpha also known as TOP2A is an enzyme that plays a critical role in DNA replication and cell division. It is a homodimeric protein with a mass of around 170 kDa and primarily localizes in the nucleus. TOP2A catalyzes the transient breaking and rejoining of double-stranded DNA necessary for resolving DNA tangles and supercoils during the replication process. TOP2A is highly expressed in proliferating cells due to its role in cell cycle progression making it a significant target in cancer research.
- Biological function summary
TOP2A is essential for chromosome condensation segregation and relief of DNA torsional stress during mitosis. It does not function in isolation but interacts with other proteins as part of the larger DNA topoisomerase complex. This complex maintains DNA integrity during cellular processes such as transcription and replication. TOP2A's activity ensures that cells can duplicate their genetic material accurately facilitating proper cell division and maintaining genomic stability.
- Pathways
TOP2A plays a significant role in the DNA replication and cell cycle regulation pathways. It interacts closely with proteins like CDC2 (cyclin-dependent kinase 1) and is regulated by cyclins which control the timing of cell cycle progression. Additionally TOP2A is involved in the DNA damage response pathway where it collaborates with proteins such as ATM (ataxia-telangiectasia mutated) emphasizing its importance in maintaining DNA structure during replication stress.
- Associated diseases and disorders
Overexpression of TOP2A is frequently associated with various cancers including breast and prostate cancer. The enzyme's increased activity promotes uncontrolled cell proliferation a hallmark of cancer development. Antitopoisomerase drugs which target this pathway are used in cancer treatments to inhibit TOP2A activity. Additionally mutations or dysregulation of TOP2A-related pathways may also contribute to chemoresistance. Understanding TOP2A interactions and regulation provides insight into its role in cancer and opens avenues for targeted therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
All lanes: Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution
Lane 1: HeLa cell lysate treated with AP at 10 µg
Lane 2: HeLa cell lysate untreated at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853), expandable thumbnail](https://content.abcam.com/products/images/topoisomerase-ii-alpha-phospho-t1343-antibody-ep1054y-ab52853--immunocytochemistry-immunofluorescence-img59977.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)")
Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Topoisomerase II alpha with purified ab52853 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.14 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853), expandable thumbnail](https://content.abcam.com/products/images/topoisomerase-ii-alpha-phospho-t1343-antibody-ep1054y-ab52853--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136713.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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