AB125297

Anti-Topoisomerase II beta/TOP2B antibody

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(4 Publications)

Rabbit Polyclonal Topoisomerase II beta/TOP2B antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

DNA topoisomerase 2-beta, TOP2B

3 Images

Lab

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB125297)

Lanes 1- 2 : Merged signal (red and green). Green - ab125297 observed at 183 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

ab125297 was shown to react with Topoisomerase II beta/TOP2B in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266340 (knockout cell lysate ab257286) was used. Wild-type HEK-293T and TOP2B knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab125297 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (ab125297) at 1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 40 µg

Lane 1:

Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-top2b-topoisomerase-ii-beta-knockout-hek-293t-cell-lysate-ab257286'>ab257286</a>)

Lane 1:

Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell pellet (<a href='/en-us/products/cell-lysates/human-top2b-topoisomerase-ii-beta-knockout-hek-293t-cell-pellet-ab279120'>ab279120</a>)

Lane 2:

TOP2B knockout HEK-293T cell lysate at 40 µg

Lane 2:

Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-top2b-topoisomerase-ii-beta-knockout-hek-293t-cell-line-ab266340'>ab266340</a>)

Predicted band size: 183 kDa

Observed band size: 183 kDa

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Lab

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB125297)

Lane 1 : Wild-type NALM-6 cell lysate (20 μg)
Lane 2 : Topoisomerase II beta/TOP2B knockout NALM-6 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : NIH/3T3 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab125297 observed at 200 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab125297 was shown to recognize Topoisomerase II beta/TOP2B when Topoisomerase II beta/TOP2B knockout samples were used, along with additional cross-reactive bands. Wild-type and Topoisomerase II beta/TOP2B knockout samples were subjected to SDS-PAGE. ab125297 and ab7291 (loading control to alpha tubulin) were diluted 1 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (ab125297)

Predicted band size: 183 kDa

false

Unknown

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB125297)

Abcam recommends using milk as the blocking agent - 5%. This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab125297 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (ab125297) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 25 µg

Lane 2:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 25 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 25 µg

Lane 4:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 183 kDa

Observed band size: 120 kDa,150 kDa,183 kDa,42 kDa,55 kDa,66 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends using milk as the blocking agent - 5%</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends using milk as the blocking agent - 5%</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II beta (TOP2B) is an essential enzyme that alters the topological states of DNA during transcription replication and chromatin remodeling. It accomplishes this by inducing transient double-strand breaks in the DNA helix to facilitate the passage of another DNA segment through this break before the enzyme reseals the break. TOP2B is also known as DNA topoisomerase (ATP-hydrolyzing) and has a molecular mass of approximately 180 kDa. The enzyme is widely expressed across various tissues but the highest levels are found in the brain and testis reflecting its vital role in these organs.

Biological function summary

Topoisomerase II beta contributes to the regulation of genetic processes such as transcription chromosome segregation and DNA repair. It is not a lone player; TOP2B often functions within a complex involving other proteins that assist in stabilizing the DNA topology changes it mediates. The enzyme's activity ensures proper chromatin dynamics particularly in neurons where it influences long-range transcription regulation essential for neural development and function.

Pathways

Topoisomerase II beta plays a significant role in DNA damage response and repair pathways interacting with intricate network proteins like RPA (Replication Protein A) and BRCA1. In addition TOP2B is involved in the transcriptional regulation pathway where it facilitates changes in chromatin structure necessary for the transcription machinery to access gene sequences. Its interactions with these proteins highlight its integration in cellular mechanisms maintaining genomic stability and regulating gene expression.

Topoisomerase II beta has associations with cancer and neurodegenerative diseases. Aberrations in TOP2B activity can lead to genomic instability contributing to oncogenesis particularly in types of leukemia and solid tumors. Elevated or dysfunctional activity of TOP2B is often observed alongside proteins like the tumor suppressor p53 an important regulator of cell cycle and apoptosis linking their combined malfunction to cancer progression. Additionally irregular TOP2B function is connected to Alzheimer's disease with evidence suggesting its involvement in neuronal death often alongside proteins like Tau further implicating its role in neurodegenerative pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. Plays a role in B-cell differentiation.

See full target information DNA topoisomerase 2-beta

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0294848 PubMed38015959

2023

Dexrazoxane does not mitigate early vascular toxicity induced by doxorubicin in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Bosman,Dustin N Krüger,Kasper Favere,Guido R Y De Meyer,Constantijn Franssen,Emeline M Van Craenenbroeck,Pieter-Jan Guns

Molecular cell 82:3794-3809.e8 PubMed36206766

2022

Calcineurin dephosphorylates topoisomerase IIβ and regulates the formation of neuronal-activity-induced DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Ilse Delint-Ramirez,Lahiri Konada,Lance Heady,Richard Rueda,Alvaro Sebastian Vaca Jacome,Eric Marlin,Charlotte Marchioni,Amir Segev,Oleg Kritskiy,Satoko Yamakawa,Andrew H Reiter,Li-Huei Tsai,Ram Madabhushi

JCI insight 5: PubMed32376803

2020

Mitochondria-dependent ferroptosis plays a pivotal role in doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Tadokoro,Masataka Ikeda,Tomomi Ide,Hiroko Deguchi,Soichiro Ikeda,Kosuke Okabe,Akihito Ishikita,Shouji Matsushima,Tomoko Koumura,Ken-Ichi Yamada,Hirotaka Imai,Hiroyuki Tsutsui

Redox biology 16:401-413 PubMed29649787

2018

Defective protein repair under methionine sulfoxide A deletion drives autophagy and ARE-dependent gene transcription.

Applications

Species

Unspecified reactive species

Steven M Pennington,Paula R Klutho,Litao Xie,Kim Broadhurst,Olha M Koval,Michael L McCormick,Douglas R Spitz,Isabella M Grumbach

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Topoisomerase II beta/TOP2B antibody

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB125297)

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB125297)

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB125297)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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