AB72334

Anti-Topoisomerase II beta/TOP2B antibody

Name: Anti-Topoisomerase II beta/TOP2B antibody (ab72334) | Abcam
Brand: Abcam Limited
SKU: AB72334
Price: 565 USD
Availability: InStock
Rating: 4 (1 reviews)

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(13 Publications)

Rabbit Polyclonal Topoisomerase II beta/TOP2B antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

DNA topoisomerase 2-beta, TOP2B

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Topoisomerase II beta with ab72334 at 1/200 (1µg/ml). Detection : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

IHC image of ab72334 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72334, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Immunoprecipitation - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

Immunoprecipitation/ Western Blot of Topoisomerase II beta/TOP2B. Lane 1 : ab72334 at 3μg/mg whole cell lysate. Lane 2 : Control IgG. ab72334 at 0.1μg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Topoisomerase II beta/TOP2B antibody (ab72334)

Predicted band size: 183 kDa

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Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

All lanes:

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (ab72334) at 0.04 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Lane 3:

Whole cell lysate from Hela cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 183 kDa

Observed band size: 210 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II beta (TOP2B) is an essential enzyme that alters the topological states of DNA during transcription replication and chromatin remodeling. It accomplishes this by inducing transient double-strand breaks in the DNA helix to facilitate the passage of another DNA segment through this break before the enzyme reseals the break. TOP2B is also known as DNA topoisomerase (ATP-hydrolyzing) and has a molecular mass of approximately 180 kDa. The enzyme is widely expressed across various tissues but the highest levels are found in the brain and testis reflecting its vital role in these organs.

Biological function summary

Topoisomerase II beta contributes to the regulation of genetic processes such as transcription chromosome segregation and DNA repair. It is not a lone player; TOP2B often functions within a complex involving other proteins that assist in stabilizing the DNA topology changes it mediates. The enzyme's activity ensures proper chromatin dynamics particularly in neurons where it influences long-range transcription regulation essential for neural development and function.

Pathways

Topoisomerase II beta plays a significant role in DNA damage response and repair pathways interacting with intricate network proteins like RPA (Replication Protein A) and BRCA1. In addition TOP2B is involved in the transcriptional regulation pathway where it facilitates changes in chromatin structure necessary for the transcription machinery to access gene sequences. Its interactions with these proteins highlight its integration in cellular mechanisms maintaining genomic stability and regulating gene expression.

Topoisomerase II beta has associations with cancer and neurodegenerative diseases. Aberrations in TOP2B activity can lead to genomic instability contributing to oncogenesis particularly in types of leukemia and solid tumors. Elevated or dysfunctional activity of TOP2B is often observed alongside proteins like the tumor suppressor p53 an important regulator of cell cycle and apoptosis linking their combined malfunction to cancer progression. Additionally irregular TOP2B function is connected to Alzheimer's disease with evidence suggesting its involvement in neuronal death often alongside proteins like Tau further implicating its role in neurodegenerative pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. Plays a role in B-cell differentiation.

See full target information DNA topoisomerase 2-beta

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Transactions of the American Clinical and Climatological Association 135:184-195 PubMed40771595

2025

ANTHRACYCLINE-INDUCED CARDIOTOXICITY: MECHANISM AND PREVENTION.

Applications

Unspecified application

Species

Unspecified reactive species

Edward T H Yeh,Hui-Ming Chang

Cardio-oncology (London, England) 11:42 PubMed40317097

2025

Prevention of Heart Failure Induced by Doxorubicin with Early Administration of Dexrazoxane (PHOENIX Study): dose response and time course of dexrazoxane-induced degradation of topoisomerase 2b.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Ming Chang,Jinn-Yuan Hsu,Chul Ahn,Edward T H Yeh

Cell reports 42:112778 PubMed37453058

2023

Spatial and clonality-resolved 3D cancer genome alterations reveal enhancer-hijacking as a potential prognostic marker for colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kyukwang Kim,Mooyoung Kim,Andrew J Lee,Sang-Hyun Song,Jun-Kyu Kang,Junghyun Eom,Gyeong Hoon Kang,Jeong Mo Bae,Sunwoo Min,Yeonsoo Kim,Yoojoo Lim,Han Sang Kim,Young-Joon Kim,Tae-You Kim,Inkyung Jung

Science advances 8:eadd3686 PubMed36351018

2022

DNA replication initiation shapes the mutational landscape and expression of the human genome.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Murat,Consuelo Perez,Alastair Crisp,Patrick van Eijk,Simon H Reed,Guillaume Guilbaud,Julian E Sale

The Journal of cell biology 221: PubMed35019938

2022

Active RB causes visible changes in nuclear organization.

Applications

Unspecified application

Species

Unspecified reactive species

Badri Krishnan,Takaaki Yasuhara,Purva Rumde,Marcello Stanzione,Chenyue Lu,Hanjun Lee,Michael S Lawrence,Lee Zou,Linda T Nieman,Ioannis Sanidas,Nicholas J Dyson

Cell communication and signaling : CCS 19:114 PubMed34784933

2021

Estradiol increases risk of topoisomerase IIβ-mediated DNA strand breaks to initiate Xp11.2 translocation renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiancheng Shi,Ning Liu,Lei Yang,Yi Chen,Yanwen Lu,Hongqian Guo,Xiaodong Han,Dongmei Li,Weidong Gan

Frontiers in cell and developmental biology 9:727836 PubMed34746125

2021

Functions of the CSB Protein at Topoisomerase 2 Inhibitors-Induced DNA Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Franciele Faccio Busatto,Sofiane Y Mersaoui,Yilun Sun,Yves Pommier,Jean-Yves Masson,Jenifer Saffi

Clinical cancer research : an official journal of the American Association for Cancer Research 27:5669-5680 PubMed34433651

2021

TOP2B Enzymatic Activity on Promoters and Introns Modulates Multiple Oncogenes in Human Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Edgar Gonzalez-Buendia,Junfei Zhao,Lu Wang,Subhas Mukherjee,Daniel Zhang,Víctor A Arrieta,Eric Feldstein,J Robert Kane,Seong Jae Kang,Catalina Lee-Chang,Aayushi Mahajan,Li Chen,Ronald Realubit,Charles Karan,Lisa Magnuson,Craig Horbinski,Stacy A Marshall,Jann N Sarkaria,Ahmed Mohyeldin,Ichiro Nakano,Mukesh Bansal,Charles D James,Daniel J Brat,Atique Ahmed,Peter Canoll,Raul Rabadan,Ali Shilatifard,Adam M Sonabend

Cell reports 32:108166 PubMed32966794

2020

BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation.

Applications

Unspecified application

Species

Unspecified reactive species

Drake S Edwards,Rohin Maganti,Jarred P Tanksley,Jie Luo,James J H Park,Elena Balkanska-Sinclair,Jinjie Ling,Scott R Floyd

Cancer chemotherapy and pharmacology 86:151-161 PubMed32607786

2020

Berberine chloride suppresses non-small cell lung cancer by deregulating Sin3A/TOP2B pathway in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Chen,Xiaofei Huang,Cheng Tao,Li Wang,Zide Chen,Xinping Li,Qiang Zeng,Min Ma,Ren Zhang,Zhengzhi Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Topoisomerase II beta/TOP2B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

Immunoprecipitation - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (AB72334)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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