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AB86307

Anti-TOR1AIP1 antibody

5

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal TOR1AIP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TOR1AIP1 aa 150-250.

View Alternative Names

LAP1, TOR1AIP1, Torsin-1A-interacting protein 1, Lamin-associated protein 1B, LAP1B

2 Images
Immunoprecipitation - Anti-TOR1AIP1 antibody (AB86307)
  • IP

Unknown

Immunoprecipitation - Anti-TOR1AIP1 antibody (AB86307)

Detection of TOR1AIP1 by Western Blot of Immunprecipitate.
ab86307, at 1 µg/ml, staining TOR1AIP1 in HeLa whole cell lysate immunoprecipitated using ab86307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TOR1AIP1 antibody (ab86307)

Predicted band size: 66 kDa

false

Western blot - Anti-TOR1AIP1 antibody (AB86307)
  • WB

Unknown

Western blot - Anti-TOR1AIP1 antibody (AB86307)

All lanes:

Western blot - Anti-TOR1AIP1 antibody (ab86307) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 66 kDa

Observed band size: 66 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TOR1AIP1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q5JTV8

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Secondary Antibodies

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Primary Antibodies

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Anti-Torsin A/DYT1 antibody

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Primary Antibodies

AB217999

Anti-HP1 gamma/CBX3 antibody [EPR19802]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR19802] (AB217999)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab86307 was affinity purified using an epitope specific to TOR1AIP1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TOR1AIP1 also known as lamina-associated polypeptide 1 (LAP1) is a mechanical protein involved in nuclear envelope structural integrity. This protein has an approximate mass of 75 kDa and is mainly found in the inner nuclear membrane. It interacts closely with components of the nuclear lamina particularly lamin A/C to contribute to the nuclear envelope architecture and maintain cellular functions.
Biological function summary

The protein known as LAP1 plays a role in modulating nuclear-cytoplasmic transport and chromatin dynamics. It is associated with the inner nuclear membrane complex and works in tandem with other nuclear lamins. This interaction is essential for processes like gene expression regulation and nuclear structure maintenance facilitating cellular response to mechanical stress.

Pathways

TOR1AIP1 participates in pathways involving nuclear envelope assembly and disassembly during cell division. It is integrated within the mitotic nuclear envelope reassembly pathway and interacts with proteins like emerin and torsinA. These proteins are essential for regulated nuclear envelope dynamics as well as maintaining proper chromatin organization during mitotic events.

Mutations in TOR1AIP1 have been linked to congenital muscular dystrophies and torsion dystonia. These conditions often arise from disrupted nuclear envelope integrity and abnormal cellular responses to mechanical stress. The close interaction with proteins such as emerin and torsinA underlines the connection between nuclear envelope dysfunction and the pathogenesis of these muscular and movement disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for nuclear membrane integrity. Induces TOR1A and TOR1B ATPase activity and is required for their location on the nuclear membrane. Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.
See full target information TOR1AIP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:2618-2633.e10 PubMed39025073

2024

The splicing factor CCAR1 regulates the Fanconi anemia/BRCA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Naoya Harada,Shuhei Asada,Lige Jiang,Huy Nguyen,Lisa Moreau,Ryan J Marina,Karen Adelman,Divya R Iyer,Alan D D'Andrea

eLife 9: PubMed33320087

2020

Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Naemi Luithle,Jelmi Uit de Bos,Ruud Hovius,Daria Maslennikova,Renard Tm Lewis,Rosemarie Ungricht,Beat Fierz,Ulrike Kutay
View all publications
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Product promise

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