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Rabbit Recombinant Monoclonal TOR1AIP1 antibody. Suitable for Dot, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Recombinant fragment - Human, Human samples.

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Images

Dot Blot - Anti-TOR1AIP1 antibody [EPR29003-34] (AB317701), expandable thumbnail
  • Western blot - Anti-TOR1AIP1 antibody [EPR29003-34] (AB317701), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TOR1AIP1 antibody [EPR29003-34] (AB317701), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [EPR29003-34] (AB317701), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [EPR29003-34] (AB317701), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotFlow Cyt (Intra)ICC/IFIHC-PWBIP
Human
Expected
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Required for nuclear membrane integrity. Induces TOR1A and TOR1B ATPase activity and is required for their location on the nuclear membrane. Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.

Alternative names

LAP1, TOR1AIP1, Torsin-1A-interacting protein 1, Lamin-associated protein 1B, LAP1B

Recommended products

Rabbit Recombinant Monoclonal TOR1AIP1 antibody. Suitable for Dot, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29003-34
Purification technique
Affinity purification Protein A
Specificity

The immunogen has been designed to the carboxyl terminus of LAP1. The region shares 100% sequence identity with LAP1B (long) and LAP1C (short) isoforms. In western blot, we were unable to detect the long isoform LAP1B (~85KDa) in the cell lines tested. We welcome any feedback from customers who have used this antibody in western blot.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TOR1AIP1 also known as lamina-associated polypeptide 1 (LAP1) is a mechanical protein involved in nuclear envelope structural integrity. This protein has an approximate mass of 75 kDa and is mainly found in the inner nuclear membrane. It interacts closely with components of the nuclear lamina particularly lamin A/C to contribute to the nuclear envelope architecture and maintain cellular functions.

Biological function summary

The protein known as LAP1 plays a role in modulating nuclear-cytoplasmic transport and chromatin dynamics. It is associated with the inner nuclear membrane complex and works in tandem with other nuclear lamins. This interaction is essential for processes like gene expression regulation and nuclear structure maintenance facilitating cellular response to mechanical stress.

Pathways

TOR1AIP1 participates in pathways involving nuclear envelope assembly and disassembly during cell division. It is integrated within the mitotic nuclear envelope reassembly pathway and interacts with proteins like emerin and torsinA. These proteins are essential for regulated nuclear envelope dynamics as well as maintaining proper chromatin organization during mitotic events.

Associated diseases and disorders

Mutations in TOR1AIP1 have been linked to congenital muscular dystrophies and torsion dystonia. These conditions often arise from disrupted nuclear envelope integrity and abnormal cellular responses to mechanical stress. The close interaction with proteins such as emerin and torsinA underlines the connection between nuclear envelope dysfunction and the pathogenesis of these muscular and movement disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Dot Blot - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701), expandable thumbnail

    Dot Blot - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701)

    TOR1AIP1 Dot Blot staining using rabbit Anti-TOR1AIP1 antibody

    Dot blot analysis of TOR1AIP1 using ab317701 at 1:1000 (0.497 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human TOR1AIP2.

    All lanes: Dot Blot - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701) at 1/1000 dilution

    Lane 1: His-tagged human TOR1AIP1 fragment

    Lane 2: His-tagged human TOR1AIP2 fragment

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701), expandable thumbnail

    Western blot - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701)

    Lanes 1-3 were freshly made and used for Western Blotting immediately to minimize protein degradation.

    Lanes 1-3 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 4-5 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/5000.

    The identity of the band at ~30KDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: HeLa transfected with siRNA specifically targeti TOR1AIP1 whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: HeLa whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 6: SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 4 - 5: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/5000 dilution

    Observed band size: 66 kDa, 36 kDa

    Exposure time: 48s

  • Flow Cytometry (Intracellular) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling TOR1AIP1 with ab317701 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling TOR1AIP1 with ab317701 at 1/500 (0.994 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing nuclear envelope staining in SH-SY5Y cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701)

    Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling TOR1AIP1 with ab317701 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Nucleus envelope staining on human melanoma (PMID: 36624187).

    The section was incubated with ab317701 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TOR1AIP1 with ab317701 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Nucleus envelope staining on human testis (PMID: 28387711).

    The section was incubated with ab317701 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [EPR29003-34] (ab317701)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TOR1AIP1 with ab317701 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Nucleus envelope staining on human tonsil.

    The section was incubated with ab317701 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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