Rabbit Recombinant Monoclonal TORC1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (AB247919), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr33812-bsa-and-azide-free-ab247919--western-blot-img105384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (AB247919)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (AB247919), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr33812-bsa-and-azide-free-ab247919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (AB247919)")
Publications
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- The Journal of investigative dermatology 136:2003-20122016Thyroid Hormones Enhance Mitochondrial Function in Human Epidermis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Vidali et. al.PubMed 27349864
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. (Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Alternative names
KIAA0616, MECT1, TORC1, WAMTP1, CRTC1, CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1
Recommended products
Rabbit Recombinant Monoclonal TORC1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3381(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247919 is the carrier-free version of Anti-TORC1 antibody [EPR3381(2)] ab109736.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TORC1 also known as Target of Rapamycin Complex 1 is a protein complex that regulates cell growth and metabolism in response to nutrients and growth factors. This complex contains proteins such as mTOR Raptor and mLST8. The mass of mTOR the catalytic subunit is approximately 289 kDa. TORC1 is mostly expressed in tissues with high metabolic activity including the liver muscle and adipose tissue. It functions as a serine/threonine kinase playing a significant role in protein synthesis autophagy and energy metabolism.
- Biological function summary
TORC1 holds a central role in cellular responses to nutrients and energy status. It is a part of the larger mechanistic target of rapamycin (mTOR) signaling pathway. This pathway influences essential cellular processes such as growth proliferation and survival. TORC1 activity increases in the presence of nutrients such as amino acids and is necessary for ribosomal biogenesis and translation initiation. It also acts as an important regulator of autophagy where its inhibition promotes autophagic degradation of cellular components balancing energy and resource availability.
- Pathways
TORC1 integrates signals from related pathways such as PI3K-AKT and AMPK. These pathways facilitate the balance between anabolic and catabolic processes. The PI3K-AKT pathway stimulates TORC1 activity promoting cell growth and proliferation while the AMPK pathway inhibits TORC1 during low energy states conserving cellular resources. Proteins like TSC1/TSC2 complex and Rheb interact with TORC1 modulating its kinase activity based on cellular and environmental conditions.
- Associated diseases and disorders
TORC1 dysregulation links to conditions like cancer and type 2 diabetes. Overactivation of TORC1 contributes to unchecked cell proliferation in various cancers. Agents that inhibit TORC1 activity such as rapamycin show potential in cancer treatment to curb tumor growth. In type 2 diabetes TORC1 is connected through insulin signaling pathways as excess TORC1 activity may lead to insulin resistance. Proteins like IRS-1 which interacts with TORC1 play a role in these metabolic and proliferative disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (ab247919), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr33812-bsa-and-azide-free-ab247919--western-blot-img105384.jpg "Western blot - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (ab247919)")
Western blot - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (ab247919)
This data was developed using Anti-TORC1 antibody [EPR3381(2)] ab109736, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TORC1 antibody [EPR3381(2)] (Anti-TORC1 antibody [EPR3381(2)] ab109736) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: Human fetal brain lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Predicted band size: 67 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (ab247919), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr33812-bsa-and-azide-free-ab247919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (ab247919)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free (ab247919)
This data was developed using Anti-TORC1 antibody [EPR3381(2)] ab109736, the same antibody clone in a different buffer formulation.Anti-TORC1 antibody [EPR3381(2)] ab109736 at 1/50 dilution staining MECT1 in paraffin-embedded Human brain tissue by Immunohistochemistry. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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