AB92477

Anti-TORC1 antibody [EPR3382]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal TORC1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 7 publications.

View Alternative Names

KIAA0616, MECT1, TORC1, WAMTP1, CRTC1, CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1

2 Images

Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (AB92477)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (AB92477)

Overlay histogram showing SHSY-5Y cells stained with ab92477 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92477 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-TORC1 antibody [EPR3382] (AB92477)

All lanes:

Western blot - Anti-TORC1 antibody [EPR3382] (ab92477) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 78 kDa

false

Carrier free

Anti-TORC1 antibody [EPR3382] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3382

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000 - 1/10000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TORC1 also known as Target of Rapamycin Complex 1 is a protein complex that regulates cell growth and metabolism in response to nutrients and growth factors. This complex contains proteins such as mTOR Raptor and mLST8. The mass of mTOR the catalytic subunit is approximately 289 kDa. TORC1 is mostly expressed in tissues with high metabolic activity including the liver muscle and adipose tissue. It functions as a serine/threonine kinase playing a significant role in protein synthesis autophagy and energy metabolism.

Biological function summary

TORC1 holds a central role in cellular responses to nutrients and energy status. It is a part of the larger mechanistic target of rapamycin (mTOR) signaling pathway. This pathway influences essential cellular processes such as growth proliferation and survival. TORC1 activity increases in the presence of nutrients such as amino acids and is necessary for ribosomal biogenesis and translation initiation. It also acts as an important regulator of autophagy where its inhibition promotes autophagic degradation of cellular components balancing energy and resource availability.

Pathways

TORC1 integrates signals from related pathways such as PI3K-AKT and AMPK. These pathways facilitate the balance between anabolic and catabolic processes. The PI3K-AKT pathway stimulates TORC1 activity promoting cell growth and proliferation while the AMPK pathway inhibits TORC1 during low energy states conserving cellular resources. Proteins like TSC1/TSC2 complex and Rheb interact with TORC1 modulating its kinase activity based on cellular and environmental conditions.

TORC1 dysregulation links to conditions like cancer and type 2 diabetes. Overactivation of TORC1 contributes to unchecked cell proliferation in various cancers. Agents that inhibit TORC1 activity such as rapamycin show potential in cancer treatment to curb tumor growth. In type 2 diabetes TORC1 is connected through insulin signaling pathways as excess TORC1 activity may lead to insulin resistance. Proteins like IRS-1 which interacts with TORC1 play a role in these metabolic and proliferative disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.. (Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

See full target information CRTC1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eado7249 PubMed39151007

2024

Magnetically attracting hydrogel reshapes iron metabolism for tissue repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yichang Xu,Feng Cai,Yidi Zhou,Jincheng Tang,Jiannan Mao,Wei Wang,Ziang Li,Liang Zhou,Yu Feng,Kun Xi,Yong Gu,Liang Chen

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2631-2652 PubMed36868861

2023

Chronic Neuronal Inactivity Utilizes the mTOR-TFEB Pathway to Drive Transcription-Dependent Autophagy for Homeostatic Up-Scaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Jingran Lin,Jiarui Li,Lu Yan,Wenwen Li,Xingzhi He,Huan Ma

Cell death discovery 8:340 PubMed35906204

2022

Reduced miR-184-3p expression protects pancreatic β-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppina E Grieco,Noemi Brusco,Daniela Fignani,Laura Nigi,Caterina Formichi,Giada Licata,Lorella Marselli,Piero Marchetti,Laura Salvini,Laura Tinti,Agnese Po,Elisabetta Ferretti,Guido Sebastiani,Francesco Dotta

Journal of nanobiotechnology 20:159 PubMed35351151

2022

SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Liu,Yang Wang,Laijin Lu,Yi Liu

Cell reports 36:109398 PubMed34289350

2021

Neuronal activity recruits the CRTC1/CREB axis to drive transcription-dependent autophagy for maintaining late-phase LTD.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Pan,Xingzhi He,Cuicui Li,Yanjun Li,Wenwen Li,Hanbin Zhang,Yang Wang,Guangjun Zhou,Jing Yang,Jiarui Li,Jing Qu,Hao Wang,Zhihua Gao,Ying Shen,Tao Li,Hailan Hu,Huan Ma

Cellular and molecular neurobiology 39:1151-1163 PubMed31270712

2019

Inhibition of Reactive Astrocytes with Fluorocitrate Ameliorates Learning and Memory Impairment Through Upregulating CRTC1 and Synaptophysin in Ischemic Stroke Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Zhang,Xianzhi Shen,Jiali Dong,Wen-Cao Liu,Min Song,Yanyun Sun,Hui Shu,Clare-Louise Towse,Wenlan Liu,Chun-Feng Liu,Xinchun Jin

Drug metabolism and disposition: the biological fa 41:1642-50 PubMed23775849

2013

Downregulation of sulfotransferase expression and activity in diseased human livers.

Applications

Unspecified application

Species

Unspecified reactive species

Emine B Yalcin,Vijay More,Karissa L Neira,Zhenqiang James Lu,Nathan J Cherrington,Angela L Slitt,Roberta S King

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TORC1 antibody [EPR3382]

Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (AB92477)

Western blot - Anti-TORC1 antibody [EPR3382] (AB92477)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise