Rabbit Recombinant Monoclonal TORC1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 5 publications.
Images
![Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (AB92477), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr3382-ab92477--flow-cytometry-intracellular-img139281.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (AB92477)")
![Western blot - Anti-TORC1 antibody [EPR3382] (AB92477), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr3382-ab92477--western-blot-img74657.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TORC1 antibody [EPR3382] (AB92477)")
Publications
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- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2631-26522023Chronic Neuronal Inactivity Utilizes the mTOR-TFEB Pathway to Drive Transcription-Dependent Autophagy for Homeostatic Up-Scaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Wang,Jingran Lin,Jiarui Li,Lu Yan,Wenwen Li,Xingzhi He,Huan MaPubMed 36868861
- Cell death discovery 8:3402022Reduced miR-184-3p expression protects pancreatic β-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuseppina E Grieco,Noemi Brusco,Daniela Fignani,Laura Nigi,Caterina Formichi,Giada Licata,Lorella Marselli,Piero Marchetti,Laura Salvini,Laura Tinti,Agnese Po,Elisabetta Ferretti,Guido Sebastiani,Francesco DottaPubMed 35906204
- Journal of nanobiotechnology 20:1592022SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Liu,Yang Wang,Laijin Lu,Yi LiuPubMed 35351151
- Cell reports 36:1093982021Neuronal activity recruits the CRTC1/CREB axis to drive transcription-dependent autophagy for maintaining late-phase LTD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Pan,Xingzhi He,Cuicui Li,Yanjun Li,Wenwen Li,Hanbin Zhang,Yang Wang,Guangjun Zhou,Jing Yang,Jiarui Li,Jing Qu,Hao Wang,Zhihua Gao,Ying Shen,Tao Li,Hailan Hu,Huan MaPubMed 34289350
- Cellular and molecular neurobiology 39:1151-11632019Inhibition of Reactive Astrocytes with Fluorocitrate Ameliorates Learning and Memory Impairment Through Upregulating CRTC1 and Synaptophysin in Ischemic Stroke Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Zhang et. al.PubMed 31270712
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Tested | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. (Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Alternative names
KIAA0616, MECT1, TORC1, WAMTP1, CRTC1, CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1
Recommended products
Rabbit Recombinant Monoclonal TORC1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3382
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TORC1 also known as Target of Rapamycin Complex 1 is a protein complex that regulates cell growth and metabolism in response to nutrients and growth factors. This complex contains proteins such as mTOR Raptor and mLST8. The mass of mTOR the catalytic subunit is approximately 289 kDa. TORC1 is mostly expressed in tissues with high metabolic activity including the liver muscle and adipose tissue. It functions as a serine/threonine kinase playing a significant role in protein synthesis autophagy and energy metabolism.
- Biological function summary
TORC1 holds a central role in cellular responses to nutrients and energy status. It is a part of the larger mechanistic target of rapamycin (mTOR) signaling pathway. This pathway influences essential cellular processes such as growth proliferation and survival. TORC1 activity increases in the presence of nutrients such as amino acids and is necessary for ribosomal biogenesis and translation initiation. It also acts as an important regulator of autophagy where its inhibition promotes autophagic degradation of cellular components balancing energy and resource availability.
- Pathways
TORC1 integrates signals from related pathways such as PI3K-AKT and AMPK. These pathways facilitate the balance between anabolic and catabolic processes. The PI3K-AKT pathway stimulates TORC1 activity promoting cell growth and proliferation while the AMPK pathway inhibits TORC1 during low energy states conserving cellular resources. Proteins like TSC1/TSC2 complex and Rheb interact with TORC1 modulating its kinase activity based on cellular and environmental conditions.
- Associated diseases and disorders
TORC1 dysregulation links to conditions like cancer and type 2 diabetes. Overactivation of TORC1 contributes to unchecked cell proliferation in various cancers. Agents that inhibit TORC1 activity such as rapamycin show potential in cancer treatment to curb tumor growth. In type 2 diabetes TORC1 is connected through insulin signaling pathways as excess TORC1 activity may lead to insulin resistance. Proteins like IRS-1 which interacts with TORC1 play a role in these metabolic and proliferative disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (ab92477), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr3382-ab92477--flow-cytometry-intracellular-img139281.jpg "Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (ab92477)")
Flow Cytometry (Intracellular) - Anti-TORC1 antibody [EPR3382] (ab92477)
Overlay histogram showing SHSY-5Y cells stained with ab92477 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92477, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Western blot - Anti-TORC1 antibody [EPR3382] (ab92477), expandable thumbnail](https://content.abcam.com/products/images/torc1-antibody-epr3382-ab92477--western-blot-img74657.jpg "Western blot - Anti-TORC1 antibody [EPR3382] (ab92477)")
Western blot - Anti-TORC1 antibody [EPR3382] (ab92477)
All lanes: Western blot - Anti-TORC1 antibody [EPR3382] (ab92477) at 1/1000 dilution
Lane 1: Fetal brain lysate at 10 µg
Lane 2: C6 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 78 kDa
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