Rabbit Polyclonal TORC2 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CREB-regulated transcription coactivator 2 aa 550 to C-terminus.
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Publications
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- Journal of Cancer 14:1011-10232023CRTC2 promotes paclitaxel resistance by inducing autophagy in ovarian cancer in part via the PI3K-AKT signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoyang Ou,Chen Peng,Yilang Wang,Shiyu Lu,Xinli Yu,Qian He,Aiqin He,Li ZhangPubMed 37151390
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CREB-regulated transcription coactivator 2 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q53ET0
Reactivity data
Select an application| IHC-P | ICC | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Alternative names
CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, TORC-2, Transducer of CREB protein 2, TORC2, CRTC2
Recommended products
Rabbit Polyclonal TORC2 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CREB-regulated transcription coactivator 2 aa 550 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CREB-regulated transcription coactivator 2 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q53ET0
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TORC2 also known as Target of Rapamycin Complex 2 is a multi-protein complex involved in regulating cell growth and survival. It consists of several subunits including mTOR RICTOR and mLST8 and has a molecular mass of 1-1.5 MDa. TORC2 acts as a serine/threonine kinase primarily phosphorylating Akt (Protein Kinase B) and other AGC family kinases. This complex is expressed in various tissues including liver muscle and brain where it influences multiple cellular functions.
- Biological function summary
The actions of TORC2 play important roles in the regulation of lipid metabolism cytoskeletal organization and stress response. This complex contributes to cell survival and proliferation by phosphorylating specific substrates that control these processes. TORC2 specifically phosphorylates the hydrophobic motif on actor proteins like Akt which is necessary for their full activation. This activity establishes TORC2 as an important regulator within numerous cellular systems that contribute to maintaining cellular integrity and homeostasis.
- Pathways
The activities of TORC2 are integral to the PI3K/Akt signaling and insulin signaling pathways. Within these pathways TORC2's phosphorylation of Akt impacts glucose metabolism protein synthesis and cell survival. It operates in parallel with TORC1 (Target of Rapamycin Complex 1) yet TORC2 responds to distinct extracellular cues highlighting its unique role. Key proteins associated with TORC2 in these pathways include PI3K upstream and Akt downstream which are essential for transmitting signals that affect cell growth and metabolic regulation.
- Associated diseases and disorders
Disruptions in TORC2 function have been associated with cancer and diabetes. The aberrant activation of TORC2 leads to unchecked cell growth and survival contributing to tumor progression in various cancer types. Additionally alterations in TORC2 activity can affect insulin signaling linking it to insulin resistance and type 2 diabetes. Proteins such as mTOR and Akt are central to these diseases with TORC2 playing a pivotal regulatory role in their pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry - Anti-TORC2 antibody (ab244419)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TORC2 (green) using ab244419 at 4 μg/ml in ICC.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody (ab244419)
Paraffin-embedded human placenta tissue stained for TORC2 using ab244419 at 1/20 dilution in immunohistochemical analysis.
Western blot - Anti-TORC2 antibody (ab244419)
All lanes: Western blot - Anti-TORC2 antibody (ab244419) at 0.4 µg/mL
All lanes: THP-1 (human monocytic leukemia cell line) whole cell lysate
Predicted band size: 73 kDa
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