Skip to main content

Rabbit Recombinant Monoclonal TORC2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282), expandable thumbnail
  • Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282), expandable thumbnail
  • Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Alternative names

CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, TORC-2, Transducer of CREB protein 2, TORC2, CRTC2

Recommended products

Rabbit Recombinant Monoclonal TORC2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR20219

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240282 is the carrier-free version of Anti-TORC2 antibody [EPR20219] ab184239.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TORC2 also known as Target of Rapamycin Complex 2 is a multi-protein complex involved in regulating cell growth and survival. It consists of several subunits including mTOR RICTOR and mLST8 and has a molecular mass of 1-1.5 MDa. TORC2 acts as a serine/threonine kinase primarily phosphorylating Akt (Protein Kinase B) and other AGC family kinases. This complex is expressed in various tissues including liver muscle and brain where it influences multiple cellular functions.

Biological function summary

The actions of TORC2 play important roles in the regulation of lipid metabolism cytoskeletal organization and stress response. This complex contributes to cell survival and proliferation by phosphorylating specific substrates that control these processes. TORC2 specifically phosphorylates the hydrophobic motif on actor proteins like Akt which is necessary for their full activation. This activity establishes TORC2 as an important regulator within numerous cellular systems that contribute to maintaining cellular integrity and homeostasis.

Pathways

The activities of TORC2 are integral to the PI3K/Akt signaling and insulin signaling pathways. Within these pathways TORC2's phosphorylation of Akt impacts glucose metabolism protein synthesis and cell survival. It operates in parallel with TORC1 (Target of Rapamycin Complex 1) yet TORC2 responds to distinct extracellular cues highlighting its unique role. Key proteins associated with TORC2 in these pathways include PI3K upstream and Akt downstream which are essential for transmitting signals that affect cell growth and metabolic regulation.

Associated diseases and disorders

Disruptions in TORC2 function have been associated with cancer and diabetes. The aberrant activation of TORC2 leads to unchecked cell growth and survival contributing to tumor progression in various cancer types. Additionally alterations in TORC2 activity can affect insulin signaling linking it to insulin resistance and type 2 diabetes. Proteins such as mTOR and Akt are central to these diseases with TORC2 playing a pivotal regulatory role in their pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (ab240282), expandable thumbnail

    Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (ab240282)

    This data was developed using Anti-TORC2 antibody [EPR20219] ab184239, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 3 minutes; Lane 3: 15 seconds.

    All lanes: Western blot - Anti-TORC2 antibody [EPR20219] (Anti-TORC2 antibody [EPR20219] ab184239) at 1/1000 dilution

    Lane 1: Human fetal heart tissue lysate at 20 µg

    Lane 2: Human fetal kidney tissue lysate at 20 µg

    Lane 3: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

    Observed band size: 78 kDa

  • Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (ab240282), expandable thumbnail

    Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (ab240282)

    This data was developed using Anti-TORC2 antibody [EPR20219] ab184239, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-3: 10 seconds; Lanes 4-9: 3 minutes.

    All lanes: Western blot - Anti-TORC2 antibody [EPR20219] (Anti-TORC2 antibody [EPR20219] ab184239) at 1/1000 dilution

    Lane 1: A20 (mouse reticulum sarcoma cell line) whole cell lysate at 20 µg

    Lane 2: CTLL-2 (mouse T lymphocyte cell line) whole cell lysate at 20 µg

    Lane 3: YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

    Lane 5: C6 (rat glial tumor cell line) whole cell lysate at 20 µg

    Lane 6: L6 (rat skeletal muscle cell line) whole cell lysate at 20 µg

    Lane 7: A-673 (human muscle Ewing's Sarcoma cell line) whole cell lysate at 20 µg

    Lane 8: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 9: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

    Observed band size: 78 kDa

  • Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (ab240282), expandable thumbnail

    Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (ab240282)

    This data was developed using Anti-TORC2 antibody [EPR20219] ab184239, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-6: 3 minutes; Lanes 7-9: 15 seconds.

    All lanes: Western blot - Anti-TORC2 antibody [EPR20219] (Anti-TORC2 antibody [EPR20219] ab184239) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse kidney lysate at 10 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Rat brain lysate at 10 µg

    Lane 5: Rat kidney lysate at 10 µg

    Lane 6: Rat spleen lysate at 10 µg

    Lane 7: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

    Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Lane 9: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

    Observed band size: 78 kDa

    Exposure times: Lanes 1-6: 3 minutes; Lanes 7-9: 15 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com