Anti-TORC2 antibody [EPR20219] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

This data was developed using ab184239, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 3 minutes; Lane 3 : 15 seconds.

All lanes:

Western blot - Anti-TORC2 antibody [EPR20219] (<a href='/en-us/products/primary-antibodies/torc2-antibody-epr20219-ab184239'>ab184239</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 78 kDa

true

• WB

Supplier Data

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

This data was developed using ab184239, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-3 : 10 seconds; Lanes 4-9 : 3 minutes.

All lanes:

Western blot - Anti-TORC2 antibody [EPR20219] (<a href='/en-us/products/primary-antibodies/torc2-antibody-epr20219-ab184239'>ab184239</a>) at 1/1000 dilution

Lane 1:

A20 (mouse reticulum sarcoma cell line) whole cell lysate at 20 µg

Lane 2:

CTLL-2 (mouse T lymphocyte cell line) whole cell lysate at 20 µg

Lane 3:

YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor cell line) whole cell lysate at 20 µg

Lane 6:

L6 (rat skeletal muscle cell line) whole cell lysate at 20 µg

Lane 7:

A-673 (human muscle Ewing's Sarcoma cell line) whole cell lysate at 20 µg

Lane 8:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 9:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 73 kDa

Observed band size: 78 kDa

true

• WB

Supplier Data

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

This data was developed using ab184239, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-6 : 3 minutes; Lanes 7-9 : 15 seconds.

All lanes:

Western blot - Anti-TORC2 antibody [EPR20219] (<a href='/en-us/products/primary-antibodies/torc2-antibody-epr20219-ab184239'>ab184239</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Lane 6:

Rat spleen lysate at 10 µg

Lane 7:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 9:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 73 kDa

Observed band size: 78 kDa

true