AB109081

Anti-TORC2 antibody [EPR3384(2)]

Name: Anti-TORC2 antibody [EPR3384(2)] (ab109081) | Abcam
Brand: Abcam Limited
SKU: AB109081
Price: 360 USD
Availability: InStock
Rating: 5 (4 reviews)

RabMAb
Recombinant
What is this?

(4 Reviews)

(11 Publications)

Rabbit Recombinant Monoclonal TORC2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

TORC2, CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, TORC-2, Transducer of CREB protein 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

ab109081 at 1/100 dilution staining Torc2 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

AbReview40219****

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

All lanes:

Western blot - Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/5000 dilution

All lanes:

Ins-1, rat insulinoma cell line, nuclear cell lysate at 1200000 Cells

Secondary

All lanes:

HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 75 kDa

true

Exposure time: 5min

This image is courtesy of an Abreview submitted by Javier Triñanes

Unknown

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

All lanes:

Western blot - Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

A673 cell lysate at 10 µg

Predicted band size: 73 kDa

false

Carrier free

Anti-TORC2 antibody [EPR3384(2)] - BSA and Azide free
660 APC

APC Anti-TORC2 antibody [EPR3384(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TORC2 antibody [EPR3384(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TORC2 antibody [EPR3384(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-TORC2 antibody [EPR3384(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TORC2 antibody [EPR3384(2)]
578 PE

PE Anti-TORC2 antibody [EPR3384(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-TORC2 antibody [EPR3384(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3384(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TORC2 also known as Target of Rapamycin Complex 2 is a multi-protein complex involved in regulating cell growth and survival. It consists of several subunits including mTOR RICTOR and mLST8 and has a molecular mass of 1-1.5 MDa. TORC2 acts as a serine/threonine kinase primarily phosphorylating Akt (Protein Kinase B) and other AGC family kinases. This complex is expressed in various tissues including liver muscle and brain where it influences multiple cellular functions.

Biological function summary

The actions of TORC2 play important roles in the regulation of lipid metabolism cytoskeletal organization and stress response. This complex contributes to cell survival and proliferation by phosphorylating specific substrates that control these processes. TORC2 specifically phosphorylates the hydrophobic motif on actor proteins like Akt which is necessary for their full activation. This activity establishes TORC2 as an important regulator within numerous cellular systems that contribute to maintaining cellular integrity and homeostasis.

Pathways

The activities of TORC2 are integral to the PI3K/Akt signaling and insulin signaling pathways. Within these pathways TORC2's phosphorylation of Akt impacts glucose metabolism protein synthesis and cell survival. It operates in parallel with TORC1 (Target of Rapamycin Complex 1) yet TORC2 responds to distinct extracellular cues highlighting its unique role. Key proteins associated with TORC2 in these pathways include PI3K upstream and Akt downstream which are essential for transmitting signals that affect cell growth and metabolic regulation.

Disruptions in TORC2 function have been associated with cancer and diabetes. The aberrant activation of TORC2 leads to unchecked cell growth and survival contributing to tumor progression in various cancer types. Additionally alterations in TORC2 activity can affect insulin signaling linking it to insulin resistance and type 2 diabetes. Proteins such as mTOR and Akt are central to these diseases with TORC2 playing a pivotal regulatory role in their pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

See full target information CRTC2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109821 PubMed38770131

2024

Hypomethylation-enhanced CRTC2 expression drives malignant phenotypes and primary resistance to immunotherapy in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruizhi Zhang,Jingjing Dai,Feifan Yao,Suiqing Zhou,Wei Huang,Jiali Xu,Kai Yu,Yining Chen,Boqiang Fan,Liren Zhang,Jing Xu,Qing Li

BMC pulmonary medicine 22:140 PubMed35410283

2022

Salt-inducible kinase 2 (SIK2) inhibitor ARN-3236 attenuates bleomycin-induced pulmonary fibrosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liangneng Zou,Dequn Hong,Kecong Li,Bingyuan Jiang

Bioengineered 12:10363-10372 PubMed34699314

2021

LINC00998 functions as a novel tumor suppressor in acute myeloid leukemia via regulating the ZFP36 ring finger protein/mammalian target of rapamycin complex 2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ximin Fang,Xiazhen Pan,Huirong Mai,Xiuli Yuan,Sixi Liu,Feiqiu Wen

Journal of neuroendocrinology 33:e12920 PubMed33314405

2020

Activation and expression of endogenous CREB-regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna I F Smith,Zidong Zhao,Jamie Walker,Stafford Lightman,Francesca Spiga

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:701-713 PubMed31654553

2019

The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ling,Haitao Huang,Yuqiu Han,Chenzhi Zhang,Xueyou Zhang,Kangchen Chen,Li Wu,Ruiqi Tang,Zhipeng Zheng,Shusen Zheng,Lanjuan Li,Baohong Wang

Journal of agricultural and food chemistry 67:10513-10520 PubMed31475823

2019

CRTC2 Is a Key Mediator of Amino Acid-Induced Milk Fat Synthesis in Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Chengjian Zhou,Xueying Li,Mengmeng Yu,Meng Li,Xuejun Gao

Thoracic cancer 9:136-141 PubMed29105369

2017

CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoqing Shi,Wei Luo,Rui Zhang,Chu Wang,Yuanyuan Zheng,Yunhua Song,Rongchun Wang,Liyan Zhang,Lihua Zhang,Weimin Li,Zhuang Luo

Journal of cellular biochemistry 119:1953-1970 PubMed28816409

2017

Genistein represses PEPCK-C expression in an insulin-independent manner in HepG2 cells and in alloxan-induced diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barilin Dkhar,Kitboklang Khongsti,Daiahun Thabah,Donkupar Syiem,Kapaettu Satyamoorthy,Bidyadhar Das

Molecular and cellular biology 37: PubMed28167604

2017

Gs-DREADD Knock-In Mice for Tissue-Specific, Temporal Stimulation of Cyclic AMP Signaling.

Applications

Species

Unspecified reactive species

Dmitry Akhmedov,Maria G Mendoza-Rodriguez,Kavitha Rajendran,Mario Rossi,Jürgen Wess,Rebecca Berdeaux

Virology journal 11:30 PubMed24529027

2014

CRTC2 enhances HBV transcription and replication by inducing PGC1α expression.

Applications

Species

Unspecified reactive species

Xiaohui Tian,Fei Zhao,Weihua Sun,Xiaoguang Zhi,Zhikui Cheng,Ming Zhou,Kanghong Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TORC2 antibody [EPR3384(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

Western blot - Anti-TORC2 antibody [EPR3384(2)] (AB109081)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise