AB203187

Anti-TORC2 (phospho S171) antibody

Name: Anti-TORC2 (phospho S171) antibody (ab203187) | Abcam
Brand: Abcam Limited
SKU: AB203187
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal TORC2 phospho S171 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CRTC2 pS171 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

TORC2, CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, TORC-2, Transducer of CREB protein 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 (phospho S171) antibody (AB203187)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 (phospho S171) antibody (AB203187)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat lung tissue, labeling TORC2 (phospho S171) using ab203187 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-TORC2 (phospho S171) antibody (AB203187)

All lanes:

Western blot - Anti-TORC2 (phospho S171) antibody (ab203187) at 1/200 dilution

Lane 1:

Human rectal carcinoma lysate

Lane 2:

Human gastric carcinoma lysate

Predicted band size: 73 kDa

Observed band size: 73 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CRTC2 pS171 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q53ET0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TORC2 also known as Target of Rapamycin Complex 2 is a multi-protein complex involved in regulating cell growth and survival. It consists of several subunits including mTOR RICTOR and mLST8 and has a molecular mass of 1-1.5 MDa. TORC2 acts as a serine/threonine kinase primarily phosphorylating Akt (Protein Kinase B) and other AGC family kinases. This complex is expressed in various tissues including liver muscle and brain where it influences multiple cellular functions.

Biological function summary

The actions of TORC2 play important roles in the regulation of lipid metabolism cytoskeletal organization and stress response. This complex contributes to cell survival and proliferation by phosphorylating specific substrates that control these processes. TORC2 specifically phosphorylates the hydrophobic motif on actor proteins like Akt which is necessary for their full activation. This activity establishes TORC2 as an important regulator within numerous cellular systems that contribute to maintaining cellular integrity and homeostasis.

Pathways

The activities of TORC2 are integral to the PI3K/Akt signaling and insulin signaling pathways. Within these pathways TORC2's phosphorylation of Akt impacts glucose metabolism protein synthesis and cell survival. It operates in parallel with TORC1 (Target of Rapamycin Complex 1) yet TORC2 responds to distinct extracellular cues highlighting its unique role. Key proteins associated with TORC2 in these pathways include PI3K upstream and Akt downstream which are essential for transmitting signals that affect cell growth and metabolic regulation.

Disruptions in TORC2 function have been associated with cancer and diabetes. The aberrant activation of TORC2 leads to unchecked cell growth and survival contributing to tumor progression in various cancer types. Additionally alterations in TORC2 activity can affect insulin signaling linking it to insulin resistance and type 2 diabetes. Proteins such as mTOR and Akt are central to these diseases with TORC2 playing a pivotal regulatory role in their pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

See full target information CRTC2 pS171

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Metabolism: clinical and experimental 152:155772 PubMed38176644

2024

GDF15 activates AMPK and inhibits gluconeogenesis and fibrosis in the liver by attenuating the TGF-β1/SMAD3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Jurado-Aguilar,Emma Barroso,Maribel Bernard,Meijian Zhang,Mona Peyman,Patricia Rada,Ángela M Valverde,Walter Wahli,Xavier Palomer,Manuel Vázquez-Carrera

JCI insight 8: PubMed37870958

2023

Comparative study in estrogen-depleted mice identifies skeletal and osteocyte transcriptomic responses to abaloparatide and teriparatide.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Lv,Jiaming Zhang,Shuang Liang,Chenhe Zhou,Dorothy Hu,Daniel J Brooks,Mary L Bouxsein,Beate Lanske,Paul Kostenuik,Francesca Gori,Roland Baron

Nutrition & metabolism 19:78 PubMed36447227

2022

Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhang,Rui Chi,Yunpeng Zhang,Yi Xie,Yunxia Liu,Qun Bao,Hengyu Lv,Bo Han,Haipeng Sun,Peng Sun

Technology in cancer research & treatment 19:1533033820962111 PubMed33000695

2020

Phosphorylation of CREB-Specific Coactivator CRTC2 at Ser238 Promotes Proliferation, Migration, and Invasion of Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Qian Liu,Hanshuo Zhang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:10056-10072 PubMed32579277

2020

Adropin regulates hepatic glucose production via PP2A/AMPK pathway in insulin-resistant hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Chen,Shen Chen,Tianran Shen,Wenqi Yang,Qian Chen,Peiwen Zhang,Yiran You,Xiaoyuan Sun,Huihui Xu,Yi Tang,Jiaxin Mi,Yan Yang,Wenhua Ling

Journal of agricultural and food chemistry 67:10513-10520 PubMed31475823

2019

CRTC2 Is a Key Mediator of Amino Acid-Induced Milk Fat Synthesis in Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Chengjian Zhou,Xueying Li,Mengmeng Yu,Meng Li,Xuejun Gao

PloS one 14:e0210930 PubMed31233505

2019

Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats.

Applications

Unspecified application

Species

Unspecified reactive species

DaoFei Song,Lei Yin,Chang Wang,XiuYing Wen

Journal of cellular biochemistry 119:1953-1970 PubMed28816409

2017

Genistein represses PEPCK-C expression in an insulin-independent manner in HepG2 cells and in alloxan-induced diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barilin Dkhar,Kitboklang Khongsti,Daiahun Thabah,Donkupar Syiem,Kapaettu Satyamoorthy,Bidyadhar Das

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TORC2 (phospho S171) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 (phospho S171) antibody (AB203187)

Western blot - Anti-TORC2 (phospho S171) antibody (AB203187)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise