Rabbit Polyclonal Torsin A/DYT1 antibody. Suitable for WB, IHC-FoFr, ICC/IF and reacts with Mouse, Rat samples. Cited in 34 publications.
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- Life science alliance 6:2023Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRahul Sharma,Martin W HetzerPubMed 37188462
- Movement disorders : official journal of the Movement Disorder Society 37:949-9612022Alpha-Synuclein is Involved in DYT1 Dystonia Striatal Synaptic Dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Ponterio,Gaia Faustini,Ilham El Atiallah,Giuseppe Sciamanna,Maria Meringolo,Annalisa Tassone,Paola Imbriani,Silvia Cerri,Giuseppina Martella,Paola Bonsi,Arianna Bellucci,Antonio PisaniPubMed 35420219
- Movement disorders : official journal of the Movement Disorder Society 36:2768-27792021Vesicular Acetylcholine Transporter Alters Cholinergic Tone and Synaptic Plasticity in DYT1 Dystonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnalisa Tassone,Giuseppina Martella,Maria Meringolo,Valentina Vanni,Giuseppe Sciamanna,Giulia Ponterio,Paola Imbriani,Paola Bonsi,Antonio PisaniPubMed 34173686
- Behavioural brain research 411:1133812021Characterization of the direct pathway in Dyt1 ΔGAG heterozygous knock-in mice and dopamine receptor 1-expressing-cell-specific Dyt1 conditional knockout mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFumiaki Yokoi,Huan-Xin Chen,Janneth Oleas,Mai Tu Dang,Hong Xing,Kelly M Dexter,Yuqing LiPubMed 34038798
- The Journal of clinical investigation :2021TorsinA restoration in a mouse model identifies a critical therapeutic window for DYT1 dystonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJay Li et. al.PubMed 33529159
- Life (Basel, Switzerland) 11:2021Effect of Gabapentin in a Neuropathic Pain Model in Mice Overexpressing Human Wild-Type or Human Mutated Torsin A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDamiana Scuteri et. al.PubMed 33445430
- Behavioural brain research 381:1124512020Improved survival and overt "dystonic" symptoms in a torsinA hypofunction mouse model.Applications:WBReactive species:MouseFumiaki Yokoi,Fangfang Jiang,Kelly Dexter,Bryan Salvato,Yuqing LiPubMed 31891745
- Neuroscience 422:1-112019A Novel Transgenic Mouse Model to Investigate the Cell-Autonomous Effects of torsinA(ΔE) Expression in Striatal Output Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPedro Gonzalez-Alegre et. al.PubMed 31669362
- Neurobiology of disease 134:1046382019Decreased number of striatal cholinergic interneurons and motor deficits in dopamine receptor 2-expressing-cell-specific Dyt1 conditional knockout mice.Applications:WBReactive species:MouseFumiaki Yokoi,Janneth Oleas,Hong Xing,Yuning Liu,Kelly M Dexter,Carly Misztal,Melinda Gerard,Iakov Efimenko,Patrick Lynch,Matthew Villanueva,Raul Alsina,Shiv Krishnaswamy,David E Vaillancourt,Yuqing LiPubMed 31618684
- EMBO molecular medicine 11:2018RGS9-2 rescues dopamine D2 receptor levels and signaling in dystonia mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Bonsi,Giulia Ponterio,Valentina Vanni,Annalisa Tassone,Giuseppe Sciamanna,Sara Migliarini,Giuseppina Martella,Maria Meringolo,Benjamin Dehay,Evelyne Doudnikoff,Venetia Zachariou,Rose E Goodchild,Nicola B Mercuri,Marcello D'Amelio,Massimo Pasqualetti,Erwan Bezard,Antonio PisaniPubMed 30552094
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | IHC-FoFr | ICC/IF | |
|---|---|---|---|
| Human | Predicted | Predicted | Predicted |
| Mouse | Tested | Expected | Tested |
| Rat | Predicted | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein with chaperone functions important for the control of protein folding, processing, stability and localization as well as for the reduction of misfolded protein aggregates. Involved in the regulation of synaptic vesicle recycling, controls STON2 protein stability in collaboration with the COP9 signalosome complex (CSN). In the nucleus, may link the cytoskeleton with the nuclear envelope, this mechanism seems to be crucial for the control of nuclear polarity, cell movement and, specifically in neurons, nuclear envelope integrity. Participates in the cellular trafficking and may regulate the subcellular location of multipass membrane proteins such as the dopamine transporter SLC6A3, leading to the modulation of dopamine neurotransmission. In the endoplasmic reticulum, plays a role in the quality control of protein folding by increasing clearance of misfolded proteins such as SGCE variants or holding them in an intermediate state for proper refolding. May have a redundant function with TOR1B in non-neural tissues (By similarity).
Alternative names
Torsin-1A, Dystonia 1 protein, Torsin ATPase 1
Recommended products
Rabbit Polyclonal Torsin A/DYT1 antibody. Suitable for WB, IHC-FoFr, ICC/IF and reacts with Mouse, Rat samples. Cited in 34 publications.
Alternative names
Torsin-1A, Dystonia 1 protein, Torsin ATPase 1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
The immunogen used to raise this antibody has 92% homology with the human Torsin A/DYT1 protein. Some customers have successfully used ab34540 in human tissue. Please contact Abcam Scientific Support for more information.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Torsin A also known as DYT1 is an endoplasmic reticulum-based AAA+ ATPase protein. It has a molecular mass of approximately 37 kDa. Torsin A is widely expressed in the central nervous system particularly in neurons. As part of its mechanical function Torsin A plays an important role in ATP binding and hydrolysis which drive conformational changes required for its activities. It is associated with maintaining proper protein folding and quality control within the endoplasmic reticulum and its activity supports membrane trafficking processes.
- Biological function summary
Torsin A participates in neuronal functions that ensure cellular homeostasis. It functions within the hetero-oligomeric complexes aiding in the assembly of cellular structures such as the nuclear envelope. Through its interaction with nuclear envelope proteins Torsin A contributes to the maintenance of nuclear integrity. This function helps to regulate the movement of lipids and proteins between the endoplasmic reticulum and other parts of the cell indicating its role in intracellular transport mechanisms.
- Pathways
This protein contributes to the dysbindin-containing biogenesis of lysosome-related organelles complex 1 (BLOC-1) pathway important in cellular trafficking processes. Torsin A interacts with other AAA+ ATPases and is closely related to LULL1 (luminal domain-like LAP1) which is a partner protein involved in similar pathways. Its function connects to cholesterol biosynthesis pathways indicating its influence on lipid homeostasis in neurons.
- Associated diseases and disorders
Mutations in the TOR1A gene that encodes Torsin A cause early-onset torsion dystonia a movement disorder affecting muscle contractions and control. Similar to DYT1 dystonia alterations in Torsin A function have links to Parkinson's disease as both conditions involve disruptions in cellular signaling pathways in neurons. Torsin A has connections to other proteins implicated in these disorders like Parkin which participates in cellular quality control impacting the same neuronal pathways disrupted in these disorders.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-Torsin A/DYT1 antibody (ab34540)
ab34540 staining Torsin A/DYT1 in Neuro2a cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab34540 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
All lanes: Western blot - Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/mL
Lane 1: Marker
Lane 2: Mouse Brain at 10 µg
Lane 3: Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg
Lane 4: Mouse Heart Mouse Tissue Lysate at 10 µg
Lane 5: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg
Lane 6: Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg
Lane 7: Western blot - Mouse brain tissue lysate - total protein (0 days) (Mouse brain tissue lysate - total protein (0 days) ab7188) at 10 µg
Lane 8: Mouse Embryonic Fibroblast 1 at 10 µg
Lane 9: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 105 kDa, 38 kDa, 65 kDa
Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
All lanes: Western blot - Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 4: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 5: Liver (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa, 65 kDa
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