Anti-Torsin A/DYT1 antibody

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Torsin A/DYT1 antibody (AB34540)

ab34540 staining Torsin A/DYT1 in Neuro2a cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab34540 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• WB

Project

Western blot - Anti-Torsin A/DYT1 antibody (AB34540)

All lanes:

Western blot - Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

Liver (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa,65 kDa

false

• WB

Ap

Western blot - Anti-Torsin A/DYT1 antibody (AB34540)

All lanes:

Western blot - Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/mL

Lane 1:

Marker

Lane 2:

Mouse Brain at 10 µg

Lane 3:

Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg

Lane 4:

Mouse Heart Mouse Tissue Lysate at 10 µg

Lane 5:

Western blot - Mouse skeletal muscle tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711'>ab29711</a>) at 10 µg

Lane 6:

Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg

Lane 7:

Western blot - Mouse brain tissue lysate - total protein (0 days) (<a href='/en-us/products/tissue-lysates/mouse-brain-tissue-lysate-total-protein-0-days-ab7188'>ab7188</a>) at 10 µg

Lane 8:

Mouse Embryonic Fibroblast 1 at 10 µg

Lane 9:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 38 kDa

Observed band size: 105 kDa,38 kDa,65 kDa

false