Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (AB247447)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Torsin A/DYT1 with purified ab76133 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS ,BSA, glycerol, and sodium azide (ab76133).

• WB

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Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (AB247447)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76133).

All lanes:

Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (<a href='/en-us/products/primary-antibodies/torsin-a-dyt1-antibody-ep2569y-ab76133'>ab76133</a>) at 1/5000 dilution

Lane 1:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Lane 4:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

Mouse lung lysate at 20 µg

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 35 kDa

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