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AB317271

Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free

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Rabbit Recombinant Multiclonal NDUFB8 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB and reacts with Human samples.

View Alternative Names

Complex I-ASHI, NADH-ubiquinone oxidoreductase ASHI subunit, CI-ASHI, NDUFB8, SDH, SDH1, SDHB, Iron-sulfur subunit of complex II, Malate dehydrogenase [quinone] iron-sulfur subunit, Ip, Complex III subunit 2, Core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCRC2, COII, COX2, COXII, MTCO2, MT-CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, ATP5A, ATP5A1, ATP5AL2, ATPM, ATP5F1A, ATP synthase F1 subunit alpha

7 Images
Flow Cytometry (Intracellular) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)

This data was developed using ab317270, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Total OXPHOS Antibody Cocktail with ab317270 at 1/500 dilution (0.1ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)

This data was developed using ab317270, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Total OXPHOS Antibody Cocktail with ab317270 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human breast carcinoma. The section was incubated with ab317270 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)

This data was developed using ab317270, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Total OXPHOS Antibody Cocktail with ab317270 at 1/500 (0.984 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

Confocal image showing mitochondrial staining in HepG2 cell line (shown in green). The counter stain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)

This data was developed using ab317270, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Total OXPHOS Antibody Cocktail with ab317270 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human liver. The section was incubated with ab317270 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)
  • WB

Supplier Data

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)

This data was developed using ab317270, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (<a href='/en-us/products/primary-antibodies/total-oxphos-antibody-cocktail-antibody-rm1134-ab317270'>ab317270</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 18 kDa,22 kDa,29 kDa,48 kDa,54 kDa

false

Exposure time: 1s

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)
  • WB

Supplier Data

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)

This data was developed using ab317270, the same antibody clone in a different buffer formulation.

Exposure time : Lanes 1-3 : 15 seconds; Lanes 4-5 : 37 seconds

All lanes:

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (<a href='/en-us/products/primary-antibodies/total-oxphos-antibody-cocktail-antibody-rm1134-ab317270'>ab317270</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa,22 kDa,29 kDa,48 kDa,54 kDa

false

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)
  • WB

Supplier Data

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271)

This data was developed using ab317270, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-COX IV antibody [EPR9442 (ABC)] - Mitochondrial Loading Control (ab202554) staining at 17kDa dilution.

All lanes:

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (<a href='/en-us/products/primary-antibodies/total-oxphos-antibody-cocktail-antibody-rm1134-ab317270'>ab317270</a>) at 1/1000 dilution

Lane 1:

MCF7 non-mitochondrial fraction at 20 µg

Lane 2:

MCF7 mitochondria fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa,22 kDa,29 kDa,48 kDa,54 kDa,36 kDa,17 kDa

false

Exposure time: 8s

  • Unconjugated

    Anti-Total OXPHOS Antibody Cocktail antibody [RM1134]

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1134

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody cocktail does cross-react with NDUFB8, SDHB, UQCRC2 and ATP5A from mouse and rat samples.

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Complementary Products

Assay Kits

AB109721

Complex I Enzyme Activity Microplate Assay Kit (Colorimetric)

Functional Studies - Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (AB109721)

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Primary Antibodies

AB14705

Anti-MTCO1 antibody [1D6E1A8]

BOND RX™ Validated|Recombinant

Western blot - Anti-MTCO1 antibody [1D6E1A8] (AB14705)

  • 5
  • 44
Primary Antibodies

AB14714

Anti-SDHB antibody [21A11AE7]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (AB14714)

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Primary Antibodies

AB14745

Anti-UQCRC2 antibody [13G12AF12BB11]

BOND RX™ Validated

Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (AB14745)

  • 5
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Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
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Reactivity data

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Product details

ab317271 is the carrier-free version of ab317270.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Total OXPHOS Antibody Cocktail targets key proteins involved in oxidative phosphorylation (OXPHOS) a critical process for ATP production in mitochondria. It includes antibodies against five complexes of the electron transport chain often known as complexes I-V. These complexes are expressed in mitochondria within almost all eukaryotic cells especially high-energy demanding tissues like muscle and brain. The molecular weight of these complex components varies but each plays an important role in transferring electrons during ATP synthesis.
Biological function summary

These complexes form the core of the electron transport chain an assembly of protein complexes embedded in the mitochondrial inner membrane. OXPHOS as part of this chain is important for cellular respiration and metabolic energy production. Proteins within these complexes work together to generate a proton gradient that drives ATP synthase activity. The efficient function of this system supports high demands of cellular energy and is essential for maintaining metabolic homeostasis.

Pathways

The Total OXPHOS Antibody Cocktail components integrate into the oxidative phosphorylation and citric acid cycle pathways. Energy transduction from NADH and FADH2 through the electron transport chain connects OXPHOS with these pathways. Cytochrome c an important protein acts as an electron shuttle between complex III and complex IV within this process highlighting its importance for proper function and energy production.

Irregularities in OXPHOS function link to disorders such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These conditions often result from mutations impacting the electron transport chain proteins disrupting ATP production and leading to energy deficits in cells. For instance mutations in NADH:ubiquinone oxidoreductase (complex I) contribute to disease development emphasizing the need for understanding these targets to develop potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFB8

Additional targets

MT-CO2,UQCRC2,SDHB,ATP5F1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com