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Rabbit Recombinant Multiclonal NDUFB8 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB and reacts with Human samples.

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Images

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271), expandable thumbnail
  • Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271), expandable thumbnail
  • Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (AB317271), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow Cyt (Intra)WBIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Additional Targets

MT-CO2, UQCRC2, SDHB, ATP5F1A

Alternative names

Complex I-ASHI, NADH-ubiquinone oxidoreductase ASHI subunit, CI-ASHI, NDUFB8

Recommended products

Rabbit Recombinant Multiclonal NDUFB8 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
RM1134
Purification technique
Affinity purification Protein A
Specificity

This antibody cocktail does cross-react with NDUFB8, SDHB, UQCRC2 and ATP5A from mouse and rat samples.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317271 is the carrier-free version of Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270.

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Total OXPHOS Antibody Cocktail targets key proteins involved in oxidative phosphorylation (OXPHOS) a critical process for ATP production in mitochondria. It includes antibodies against five complexes of the electron transport chain often known as complexes I-V. These complexes are expressed in mitochondria within almost all eukaryotic cells especially high-energy demanding tissues like muscle and brain. The molecular weight of these complex components varies but each plays an important role in transferring electrons during ATP synthesis.

Biological function summary

These complexes form the core of the electron transport chain an assembly of protein complexes embedded in the mitochondrial inner membrane. OXPHOS as part of this chain is important for cellular respiration and metabolic energy production. Proteins within these complexes work together to generate a proton gradient that drives ATP synthase activity. The efficient function of this system supports high demands of cellular energy and is essential for maintaining metabolic homeostasis.

Pathways

The Total OXPHOS Antibody Cocktail components integrate into the oxidative phosphorylation and citric acid cycle pathways. Energy transduction from NADH and FADH2 through the electron transport chain connects OXPHOS with these pathways. Cytochrome c an important protein acts as an electron shuttle between complex III and complex IV within this process highlighting its importance for proper function and energy production.

Associated diseases and disorders

Irregularities in OXPHOS function link to disorders such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These conditions often result from mutations impacting the electron transport chain proteins disrupting ATP production and leading to energy deficits in cells. For instance mutations in NADH:ubiquinone oxidoreductase (complex I) contribute to disease development emphasizing the need for understanding these targets to develop potential therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271), expandable thumbnail

    Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271)

    This data was developed using Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270, the same antibody clone in a different buffer formulation.

    Exposure time: Lanes 1-3: 15 seconds; Lanes 4-5: 37 seconds

    All lanes: Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 18 kDa, 22 kDa, 29 kDa, 48 kDa, 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271)

    This data was developed using Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Total OXPHOS Antibody Cocktail with Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270 at 1/500 (0.984 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing mitochondrial staining in HepG2 cell line (shown in green). The counter stain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271), expandable thumbnail

    Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271)

    This data was developed using Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-COX IV antibody [EPR9442 (ABC)] - Mitochondrial Loading Control (Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554) staining at 17kDa dilution.

    All lanes: Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270) at 1/1000 dilution

    Lane 1: MCF7 non-mitochondrial fraction at 20 µg with NFDM/TBST

    Lane 2: MCF7 mitochondria fraction at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 18 kDa, 22 kDa, 29 kDa, 48 kDa, 54 kDa, 36 kDa, 17 kDa

    Exposure time: 8s

  • Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271), expandable thumbnail

    Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271)

    This data was developed using Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Human kidney tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 18 kDa, 22 kDa, 29 kDa, 48 kDa, 54 kDa

    Exposure time: 1s

  • Flow Cytometry (Intracellular) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271)

    This data was developed using Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Total OXPHOS Antibody Cocktail with Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270 at 1/500 dilution (0.1ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271)

    This data was developed using Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Total OXPHOS Antibody Cocktail with Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human breast carcinoma. The section was incubated with Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free (ab317271)

    This data was developed using Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Total OXPHOS Antibody Cocktail with Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human liver. The section was incubated with Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] ab317270 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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