AB314043

Anti-TOX antibody [BLR212K] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal TOX antibody. Suitable for Flow Cyt (Intra), IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TOX aa 50-100.

View Alternative Names

KIAA0808, TOX, Thymocyte selection-associated high mobility group box protein TOX, Thymus high mobility group box protein TOX

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

FFPE section of MOLT-4 (Human lymphoblastic leukemia cell line) cells. Antibody : Standard version of ab314043 at 1/100 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG.

Flow Cytometry (Intracellular) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Detection of human TOX in Jurkat cells by Flow Cytometry (Intracellular). Antibody : Standard version of ab314043 (shaded) or isotype control (unshaded). Secondary : DyLight® 488-conjugated goat anti-rabbit IgG.

Supplier Data

Immunoprecipitation - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Samples : Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from MOLT-4 cells prepared using NETN lysis buffer. Antibodies : Rabbit antiTOX recombinant monoclonal antibody [BL-178-12C7] (A700-212 lot 1) used for IP at 20 µl/mg lysate. TOX was also immunoprecipitated by a second antibody against a different epitope of TOX (Antibody 2). For blotting immunoprecipitated TOX, A700-212 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

FFPE section of BW5147.3 (Mouse cell line) cells. Antibody : Standard version of ab314043. Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Western blot - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Samples : Whole cell lysate (10 µg) from MOLT-4, HEK293T, Jurkat, HeLa, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody : Rabbit anti-TOX recombinant monoclonal antibody [BLR212K] (A700-212 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-TOX antibody [BLR212K] - BSA free (ab314043) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 5:

CCRF-CEM (Human lymphoblastoid cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Supplier Data

Western blot - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Samples : Whole cell lysate (10 µg) from NIH 3T3, BW5147.3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-TOX recombinant monoclonal antibody [BLR212K] (A700-212 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A

All lanes:

Western blot - Anti-TOX antibody [BLR212K] - BSA free (ab314043) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

BW5147.3 (Mouse cell line) whole cell lysate at 10 µg

Lane 3:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 10 µg

Lane 4:

EL4 (Mouse T-cell lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR212K

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TOX aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O94900

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "0.1 µl per 1 x 10^6 cells.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "20 µl/1 mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TOX gene encodes the TOX protein also known as Thymocyte selection-associated high mobility group (HMG) box protein with a molecular weight of approximately 57 kDa. TOX contains a DNA-binding HMG box domain and operates mechanically as a transcription factor. It expresses mainly in the thymus where it plays a significant role in T cell development. TOX also exhibits expression in lymphoid tissues including the spleen and lymph nodes.

Biological function summary

TOX affects T cell maturation and development particularly during thymocyte selection. The protein contributes critically to the decision-making process that determines whether thymocytes become CD4+ or CD8+ T cells. TOX does not assemble into a known complex but interacts with other developmental transcription factors to influence gene expression patterns in immature T cells. Its expression is tightly regulated to ensure proper immune system development.

Pathways

TOX influences key signal transduction pathways that guide T cell lineage development. It integrates into the T cell receptor (TCR) signaling pathway impacting the expression of genes essential for T cell differentiation. TOX interacts with signaling proteins like Notch1 which affects developmental fate decisions in thymocytes. The balance of these pathways maintains immunity homeostasis and correct immune responses.

TOX links to conditions involving immune dysregulation such as autoimmune diseases and certain lymphomas. Aberrant expression or dysfunction of TOX may contribute to improper immune cell development leading to pathologies like T cell leukemia. In autoimmune diseases altered TOX levels may impact self-tolerance involving proteins such as FOXP3 which governs regulatory T cell function. Understanding TOX's role in these diseases broadens insights into potential therapeutic targets for immune-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator with a major role in neural stem cell commitment and corticogenesis as well as in lymphoid cell development and lymphoid tissue organogenesis (By similarity). Binds to GC-rich DNA sequences in the proximity of transcription start sites and may alter chromatin structure, modifying access of transcription factors to DNA. During cortical development, controls the neural stem cell pool by inhibiting the switch from proliferative to differentiating progenitors. Beyond progenitor cells, promotes neurite outgrowth in newborn neurons migrating to reach the cortical plate. May activate or repress critical genes for neural stem cell fate such as SOX2, EOMES and ROBO2 (By similarity). Plays an essential role in the development of lymphoid tissue-inducer (LTi) cells, a subset necessary for the formation of secondary lymphoid organs : peripheral lymph nodes and Peyer's patches. Acts as a developmental checkpoint and regulates thymocyte positive selection toward T cell lineage commitment. Required for the development of various T cell subsets, including CD4-positive helper T cells, CD8-positive cytotoxic T cells, regulatory T cells and CD1D-dependent natural killer T (NKT) cells. Required for the differentiation of common lymphoid progenitors (CMP) to innate lymphoid cells (ILC) (By similarity). May regulate the NOTCH-mediated gene program, promoting differentiation of the ILC lineage. Required at the progenitor phase of NK cell development in the bone marrow to specify NK cell lineage commitment (By similarity) (PubMed : 21126536). Upon chronic antigen stimulation, diverts T cell development by promoting the generation of exhaustive T cells, while suppressing effector and memory T cell programming. May regulate the expression of genes encoding inhibitory receptors such as PDCD1 and induce the exhaustion program, to prevent the overstimulation of T cells and activation-induced cell death (By similarity).

See full target information TOX

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TOX antibody [BLR212K] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Flow Cytometry (Intracellular) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Immunoprecipitation - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Western blot - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Western blot - Anti-TOX antibody [BLR212K] - BSA free (AB314043)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise