Rabbit Recombinant Monoclonal TOX antibody. Suitable for Flow Cyt (Intra), IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TOX aa 50-100.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
Flow Cyt (Intra) | IHC-P | IP | WB | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes 0.1 µl per 1 x 10^6 cells. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Species Mouse | Dilution info 1/100 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes 20 µl/1 mg lysate |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Transcriptional regulator with a major role in neural stem cell commitment and corticogenesis as well as in lymphoid cell development and lymphoid tissue organogenesis (By similarity). Binds to GC-rich DNA sequences in the proximity of transcription start sites and may alter chromatin structure, modifying access of transcription factors to DNA. During cortical development, controls the neural stem cell pool by inhibiting the switch from proliferative to differentiating progenitors. Beyond progenitor cells, promotes neurite outgrowth in newborn neurons migrating to reach the cortical plate. May activate or repress critical genes for neural stem cell fate such as SOX2, EOMES and ROBO2 (By similarity). Plays an essential role in the development of lymphoid tissue-inducer (LTi) cells, a subset necessary for the formation of secondary lymphoid organs: peripheral lymph nodes and Peyer's patches. Acts as a developmental checkpoint and regulates thymocyte positive selection toward T cell lineage commitment. Required for the development of various T cell subsets, including CD4-positive helper T cells, CD8-positive cytotoxic T cells, regulatory T cells and CD1D-dependent natural killer T (NKT) cells. Required for the differentiation of common lymphoid progenitors (CMP) to innate lymphoid cells (ILC) (By similarity). May regulate the NOTCH-mediated gene program, promoting differentiation of the ILC lineage. Required at the progenitor phase of NK cell development in the bone marrow to specify NK cell lineage commitment (By similarity) (PubMed:21126536). Upon chronic antigen stimulation, diverts T cell development by promoting the generation of exhaustive T cells, while suppressing effector and memory T cell programming. May regulate the expression of genes encoding inhibitory receptors such as PDCD1 and induce the exhaustion program, to prevent the overstimulation of T cells and activation-induced cell death (By similarity).
KIAA0808, KIAA0808, TOX, Thymocyte selection-associated high mobility group box protein TOX, Thymus high mobility group box protein TOX
Rabbit Recombinant Monoclonal TOX antibody. Suitable for Flow Cyt (Intra), IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TOX aa 50-100.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
BLR212K
Blue Ice
Up to 12 months
+4°C
+4°C
This supplementary information is collated from multiple sources and compiled automatically.
The TOX gene encodes the TOX protein also known as Thymocyte selection-associated high mobility group (HMG) box protein with a molecular weight of approximately 57 kDa. TOX contains a DNA-binding HMG box domain and operates mechanically as a transcription factor. It expresses mainly in the thymus where it plays a significant role in T cell development. TOX also exhibits expression in lymphoid tissues including the spleen and lymph nodes.
TOX affects T cell maturation and development particularly during thymocyte selection. The protein contributes critically to the decision-making process that determines whether thymocytes become CD4+ or CD8+ T cells. TOX does not assemble into a known complex but interacts with other developmental transcription factors to influence gene expression patterns in immature T cells. Its expression is tightly regulated to ensure proper immune system development.
TOX influences key signal transduction pathways that guide T cell lineage development. It integrates into the T cell receptor (TCR) signaling pathway impacting the expression of genes essential for T cell differentiation. TOX interacts with signaling proteins like Notch1 which affects developmental fate decisions in thymocytes. The balance of these pathways maintains immunity homeostasis and correct immune responses.
TOX links to conditions involving immune dysregulation such as autoimmune diseases and certain lymphomas. Aberrant expression or dysfunction of TOX may contribute to improper immune cell development leading to pathologies like T cell leukemia. In autoimmune diseases altered TOX levels may impact self-tolerance involving proteins such as FOXP3 which governs regulatory T cell function. Understanding TOX's role in these diseases broadens insights into potential therapeutic targets for immune-related diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
FFPE section of BW5147.3 (Mouse cell line) cells.
Antibody: Standard version of ab314043.
Secondary: HRP-conjugated goat anti-rabbit IgG.
FFPE section of MOLT-4 (Human lymphoblastic leukemia cell line) cells.
Antibody: Standard version of ab314043 at 1/100 dilution.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human TOX in Jurkat cells by Flow Cytometry (Intracellular).
Antibody: Standard version of ab314043 (shaded) or isotype control (unshaded).
Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.
Samples:Whole cell lysate (10 µg) from MOLT-4, HEK293T, Jurkat, HeLa, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody:Rabbit anti-TOX recombinant monoclonal antibody [BLR212K] (A700-212 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
All lanes: Western blot - Anti-TOX antibody [BLR212K] - BSA free (ab314043) at 1/1000 dilution
Lane 1: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 5: CCRF-CEM (Human lymphoblastoid cell line) whole cell lysate at 10 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Samples:Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from MOLT-4 cells prepared using NETN lysis buffer. Antibodies:Rabbit antiTOX recombinant monoclonal antibody [BL-178-12C7] (A700-212 lot 1) used for IP at 20 µl/mg lysate. TOX was also immunoprecipitated by a second antibody against a different epitope of TOX (Antibody 2). For blotting immunoprecipitated TOX, A700-212 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Samples:Whole cell lysate (10 µg) from NIH 3T3, BW5147.3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TOX recombinant monoclonal antibody [BLR212K] (A700-212 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A
All lanes: Western blot - Anti-TOX antibody [BLR212K] - BSA free (ab314043) at 1/1000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 2: BW5147.3 (Mouse cell line) whole cell lysate at 10 µg
Lane 3: TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 10 µg
Lane 4: EL4 (Mouse T-cell lymphoma cell line) whole cell lysate at 10 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
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