Anti-TOX antibody [NAN448B]

• WB

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Western blot - Anti-TOX antibody [NAN448B] (AB237009)

All lanes:

Western blot - Anti-TOX antibody [NAN448B] (ab237009) at 1/200 dilution

Lane 1:

MOLT 4 cell line at 100 µg

Lane 2:

MOLT 4 KO cell line at 100 µg

Predicted band size: 58 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded human fetal liver tissue stained for TOX using ab237009 at 1/300 dilution in immunohistochemical analysis.

Antigen retrieval : ER2 for 20 minutes.

Incubated with primary antibody for 30 minutes.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-TOX antibody [NAN448B] (AB237009)

To confirm that NAN448B mAb recognized human TOX protein and showed no cross-reactivity to other TOX family members, immunohistochemistry on frozen cytospins preparations of TOX1 ,TOX2 ,TOX3 and TOX4 expressed in HEK293T cells was performed. Anti-myc and anti-GFP antibodies were used as positive controls.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Immunofluorescent analysis of paraffin-embedded human tonsil tissue labelling TOX with ab237009 at 1/50 dilution (Red). DAPI is stained in blue and Ki67 is stained in green.

Antigen retrieval : 20 minutes ER2 (Tris-EDTA)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded human kidney tissue stained for TOX using ab237009 at 1/300 dilution in immunohistochemical analysis.

Antigen retrieval : ER2 for 20 minutes.

Incubated with primary antibody for 30 minutes.

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

The BOND™ Polymer Refine Detection System on the BOND RX Automated Stainer (DS9800, Leica Biosystems, Wetzlar, Germany) was used for immunohistochemical labelling of formalin-fixed, paraffin-embedded (FFPE) tissues. The protocol includes a peroxide block, post-primary reagent, polymer detection, DAB chromogen, and haematoxylin counterstain, all automated to minimize variability. Antigen retrieval was performed by heat-induced epitope retrieval (HIER) for 20 minutes in Tris-EDTA buffer (BOND Epitope Retrieval Solution 2, AR9640, Leica Biosystems, Wetzlar, Germany). The sequence comprised peroxide block (10 min), primary antibody incubation (30 min), post-primary (10 min), an anti-rat step (1 : 300 dilution, Vector Laboratories, California, USA; 20 min), polymer (10 min), DAB (10 min), and haematoxylin (8 min).

For mouse tissues, antigen retrieval was performed using high pH buffers (Dako, Agilent), followed by blocking of endogenous peroxidase activity with 3% hydrogen peroxide. Slides were then incubated with the primary antibody. Antigen/antibody complexes detection was carried out using a horseradish peroxidase-conjugated visualization system (Novolink Polymer, Leica). Staining was developed with 3,3′-diaminobenzidine (EnVision FLEX DAB Enhancer, Dako, Agilent), and nuclei were counterstained with hematoxylin (FLEX Hematoxylin, SM806, Dako, Agilent). Images were acquired using an Axiocam CCD camera (Zeiss) and processed with ZEN 2.1 software and Photoshop 9.0.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded human testicle tissue stained for TOX using ab237009 at 1/300 dilution in immunohistochemical analysis.

Antigen retrieval : ER2 for 20 minutes.

Incubated with primary antibody for 30 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded human tonsil tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis.

Antigen retrieval : 20 minutes ER2 (Tris-EDTA)

Incubated with primary antibody for 30 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Immunofluorescent analysis of paraffin-embedded human tonsil tissue labelling TOX with ab237009 at 1/50 dilution (Red). DAPI is stained in blue and Ki67 is stained in green.

Antigen retrieval : 20 minutes ER2 (Tris-EDTA)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded human tonsil tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis.

Antigen retrieval : 20 minutes ER2 (Tris-EDTA)

Incubated with primary antibody for 30 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded mouse thymus tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.

Antigen retrieval : pH high

Incubated with primary antibody for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded mouse brain tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.

Antigen retrieval : pH high

Incubated with primary antibody for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse spleen staining TOX1 with purified ab237009 at 1 : 100 for 20 mins. Antigen retrival was performed using a high pH.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded mouse pancreas tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.

Antigen retrieval : pH high

Incubated with primary antibody for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded mouse retina tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.

Antigen retrieval : pH high

Incubated with primary antibody for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded mycosis fungoide tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis. CD8 is stained in red.

Antigen retrieval : 20 minutes ER2 (Tris-EDTA).

Incubated with primary antibody for 30 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (AB237009)

Paraffin-embedded Nodular Lymphocyte predominant Hodgkin Lymphoma (NLPHL) tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis. PD1 is stained in red.

Antigen retrieval : 20 minutes ER2 (Tris-EDTA).

Incubated with primary antibody for 30 minutes.