AB151548

Anti-TOX3 antibody [EPR6173(2)(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal TOX3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CAGF9, TNRC9, TOX3, TOX high mobility group box family member 3, CAG trinucleotide repeat-containing gene F9 protein, Trinucleotide repeat-containing gene 9 protein

2 Images

Flow Cytometry (Intracellular) - Anti-TOX3 antibody [EPR6173(2)(B)] (AB151548)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TOX3 antibody [EPR6173(2)(B)] (AB151548)

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling TOX3 with ab151548 at 1/10 dilution (red), compared to a rabbit IgG negative control antibody (green).

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Western blot - Anti-TOX3 antibody [EPR6173(2)(B)] (AB151548)

All lanes:

Western blot - Anti-TOX3 antibody [EPR6173(2)(B)] (ab151548) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

MDA-MB-435 cell lysate at 10 µg

Lane 4:

Fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

false

Carrier free

Anti-TOX3 antibody [EPR6173(2)(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6173(2)(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TOX3 also known as TOX high mobility group box family member 3 is a transcription factor with a significant role in chromatin remodeling and gene expression regulation. It has a molecular mass of approximately 60 kDa. TOX3 mainly gets expressed in neuronal tissues but it is also found in breast tissue and embryonic development sites. As a transcription factor TOX3 influences cellular processes by binding to DNA and interacting with other proteins to control the transcription of target genes. Through these interactions TOX3 can activate or suppress specific genes in different contexts.

Biological function summary

TOX3 interacts with other components to influence neural development and neuroplasticity. It does not strictly operate as a lone factor; rather it associates with protein complexes for its functions. This protein plays an important part in the survival and function of neurons. In breast tissue TOX3 may influence cellular architecture and receptor signaling pathways that are important for tissue differentiation and maintenance. The dynamic role of TOX3 in various tissues marks it as a versatile regulator essential for a broad range of cellular processes.

Pathways

TOX3 integrates into the Wnt signaling and estrogen receptor pathways. These pathways are critical for developmental processes and cell fate determination. Within the Wnt signaling pathway TOX3 interacts with downstream effectors to modulate the transcriptional outcomes. In relation to the estrogen receptor pathway TOX3 links with other transcription factors such as FOXA1 to regulate target genes in hormone-responsive tissues. These interactions place TOX3 as a significant intermediary in conveying signals from external cues to drive specific cellular outcomes.

TOX3 links closely to breast cancer and neurodegenerative diseases like Alzheimer's disease. In breast cancer alterations in TOX3 expression levels correlate with disease progression and prognosis often in connection with altered functions of estrogen receptors and BRCA1. In neurodegenerative contexts TOX3 participates in pathways affecting neuronal survival and may share mechanistic links with proteins like APP linked to Alzheimer's disease pathology. The dual involvement in cancer and neurological diseases highlights TOX3 as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator of the p300/CBP-mediated transcription complex. Activates transactivation through cAMP response element (CRE) sites. Protects against cell death by inducing antiapoptotic and repressing pro-apoptotic transcripts. Stimulates transcription from the estrogen-responsive or BCL-2 promoters. Required for depolarization-induced transcription activation of the C-FOS promoter in neurons. Associates with chromatin to the estrogen-responsive C3 promoter region.

See full target information TOX3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

Cell biology international 46:1074-1088 PubMed35347804

2022

TOX3 regulates the proliferation and apoptosis of colorectal cancer by downregulating RhoB via the activation of the MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Wei Wu,Hailiang Liang,Jiejing Chen,Xiaoqiang Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TOX3 antibody [EPR6173(2)(B)]

Flow Cytometry (Intracellular) - Anti-TOX3 antibody [EPR6173(2)(B)] (AB151548)

Western blot - Anti-TOX3 antibody [EPR6173(2)(B)] (AB151548)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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